Deeptha Kumaraswamy

Assistant Scientist at Genesis Biotechnology Group
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Contact Information
us****@****om
(386) 825-5501
Location
New York City Metropolitan Area
Languages
  • English Full professional proficiency
  • Tamil Native or bilingual proficiency
  • Hindi Native or bilingual proficiency

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Credentials

  • Spotfire - The Complete TIBCO Spotfire Course
    Udemy
    Feb, 2022
    - Nov, 2024
  • Developing Adaptable Employees
    LinkedIn
    Jan, 2020
    - Nov, 2024
  • Medical Billing & Coding
    Rutgers University

Experience

  • Genesis Biotechnology Group
    • United States
    • Biotechnology Research
    • 100 - 200 Employee
    • Assistant Scientist
      • Sep 2021 - Present

      • Responsible for all aspects of these projects, including initiation of novel ideas and approaches, detailed written proposals, experimental design and implementation, data analysis and interpretation, and the final presentation • Protein expression optimization in E.coli using various tag screening • Multiple gene expression (co-transfection and co-infection) in baculoviral/insect and suspension mammalian cells • Detergent screening and protein solubilizing strategies in native and denaturing conditions • Membrane protein purification and functional integrity checking of the synthesized proteins • High molecular weight transmembrane domain protein purification using IMAC, IEX, and SEC columns in single and multi-D modules using NGC systems (Bio-Rad) • Transfection-grade DNA purification and isolation from E.coli, insect, and mammalian cells • Primer/plasmid designing and restriction cloning (RE) and gene synthesis • Invivo SRE-cell-based assay to access protein function and integrity • NanoBRET, alpha screen, TR-FRET, and fluorescence polarization assay using PerkinElmer Envision to study target engagement • CFX Maestro Software for CFX Real-Time PCR Instruments & TIBCO Spotfire® Data Visualization and Analytics • Chromlab software compatible with all NGC systems Show less

  • Rutgers University
    • United States
    • Higher Education
    • 700 & Above Employee
    • Laboratory Researcher IV
      • May 2018 - Sep 2021

      • RNA extraction and cDNA synthesis using PCR and qRT-PCR from various tissue using column and traditional method • Optimize protocols for storing and processing human fecal samples for microbiota composition characterization using next-generation sequencing and preparation of encapsulated fecal microbiota (GMP standard) for fecal microbiota transplantation (FMT) clinical trial. • Short Chain Fatty Acids (SCFA) content analysis using gas chromatography/mass spectrometry (GC/MS) system (Agilent). • Thin layer chromatography (TLC) for lipid profiling • Skilled in using Agilent technologies GC-MS for comprehensive analysis of each lipid component from various matrixes (insects, fish, plant and animal tissues, human and rodent feces, fermentation liquid, human and animal cell lines) • Performed assays for detecting enzyme activity by absorbance, fluorescence, or chemiluminescence (UV/VIS spectroscopy) • Agarose gel electrophoresis and Western blot analysis • ELISA for linearity-of-dilution experiments • Measurement of isotope-labeled samples by Scintillation counting (Beckman Coulter) • Agilent Chemstation® software and Thermo Scientific Lab Execution System (LES) platforms • Database and reporting tools like Microsoft Word, Excel, Access, and PowerPoint. • Adobe Photoshop and Illustrator • EndNote, GraphPad Prism, SPSS Show less

  • Rutgers University–New Brunswick
    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Assistant
      • Jan 2018 - May 2018

      • Cell line maintenance, harvesting, and protein and lipid extraction • DNA purification and quantification • Lipid analysis in various tissues using thin-layer chromatography (TLC) and GC/MS • qRT-PCR, ELISA, and other wet lab analysis • Dosing lab rodents by oral gavage, IV and IP injections, hair/shaving, tumor measurement, and sample collection (urine and feces) • Proficiency in techniques that includes repeated and terminal blood collection from animals • Perform tail and ear PCR genotyping, database collection, and recording Show less

Education

  • Annamalai University
    Master's degree, Biochemistry
    2002 - 2004
  • Madurai Kamaraj University
    Bachelor's degree, Biochemistry
    2000 - 2002
  • Rutgers University-New Brunswick
    Medical Billing & Coding Program
    2011 -

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