Deepa Vadakkan
Cell Therapy Supervisor at Gates Biomanufacturing Facility- Claim this Profile
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Bio
Experience
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Gates Biomanufacturing Facility
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United States
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Biotechnology Research
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1 - 100 Employee
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Cell Therapy Supervisor
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Jun 2021 - Present
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Professional Research Assistant
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Apr 2016 - Jun 2021
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ATCC
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United States
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Biotechnology Research
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400 - 500 Employee
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Biologist/Virologist
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Sep 2010 - Jun 2014
• Growth and authentication of viruses in tissue culture including influenza A, respiratory syncytial virus, dengue virus, and metapneumovirus. • Growth and authentication of influenza A and B viruses in embryonated chicken eggs. • Performance of quality control assays including hemagglutination assays, TCID50, CEID50, and immunofluorescence assays. • Growth and preservation of adherent cell lines. • Performance of bacterial techniques including plasmid transformation, plasmid… Show more • Growth and authentication of viruses in tissue culture including influenza A, respiratory syncytial virus, dengue virus, and metapneumovirus. • Growth and authentication of influenza A and B viruses in embryonated chicken eggs. • Performance of quality control assays including hemagglutination assays, TCID50, CEID50, and immunofluorescence assays. • Growth and preservation of adherent cell lines. • Performance of bacterial techniques including plasmid transformation, plasmid extraction, and restriction enzyme digestion. • Performance of molecular biology techniques including nucleic acid extraction, PCR, realtime PCR, primer design, and sequence analysis. • Training of new employees in various techniques. • Experience in using QAD, SharePoint, and Microsoft Office. • Maintenance of laboratory equipment and inventory management of laboratory supplies. • Performance of laboratory techniques under ISO 9001:2008 requirements. • Experience in record keeping that includes laboratory notebooks and production documentation. • Ability to accurately follow standard operating procedures (SOPs). Show less • Growth and authentication of viruses in tissue culture including influenza A, respiratory syncytial virus, dengue virus, and metapneumovirus. • Growth and authentication of influenza A and B viruses in embryonated chicken eggs. • Performance of quality control assays including hemagglutination assays, TCID50, CEID50, and immunofluorescence assays. • Growth and preservation of adherent cell lines. • Performance of bacterial techniques including plasmid transformation, plasmid… Show more • Growth and authentication of viruses in tissue culture including influenza A, respiratory syncytial virus, dengue virus, and metapneumovirus. • Growth and authentication of influenza A and B viruses in embryonated chicken eggs. • Performance of quality control assays including hemagglutination assays, TCID50, CEID50, and immunofluorescence assays. • Growth and preservation of adherent cell lines. • Performance of bacterial techniques including plasmid transformation, plasmid extraction, and restriction enzyme digestion. • Performance of molecular biology techniques including nucleic acid extraction, PCR, realtime PCR, primer design, and sequence analysis. • Training of new employees in various techniques. • Experience in using QAD, SharePoint, and Microsoft Office. • Maintenance of laboratory equipment and inventory management of laboratory supplies. • Performance of laboratory techniques under ISO 9001:2008 requirements. • Experience in record keeping that includes laboratory notebooks and production documentation. • Ability to accurately follow standard operating procedures (SOPs). Show less
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Heartland Community College
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United States
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Higher Education
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300 - 400 Employee
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Adjunct Lecturer– Microbiology
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Aug 2007 - May 2009
• Worked as a part time faculty in the department of Biology. • Taught Microbiology, Anatomy and Physiology for undergraduate students. • Worked as a part time faculty in the department of Biology. • Taught Microbiology, Anatomy and Physiology for undergraduate students.
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University of Colorado Health Science Center (UCHSC)
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Denver, Colorado, United States
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Research Associate
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Feb 2002 - Dec 2004
• Co-author of a paper published in the Journal of Acquired Immune Deficiency Syndrome. • Played a key role in a multi centric Shingles Prevention study by Merck using FDA regulations and SOPs. • Experience in flow cytometry, Elispot, and cytokine analysis. • Knowledge of cell culture and tissue culture techniques. • Knowledge of cell line maintenance and cryopreservation of cells. • Experience with separation of PMNLs by histopaque techniques. • Experience in the measurements… Show more • Co-author of a paper published in the Journal of Acquired Immune Deficiency Syndrome. • Played a key role in a multi centric Shingles Prevention study by Merck using FDA regulations and SOPs. • Experience in flow cytometry, Elispot, and cytokine analysis. • Knowledge of cell culture and tissue culture techniques. • Knowledge of cell line maintenance and cryopreservation of cells. • Experience with separation of PMNLs by histopaque techniques. • Experience in the measurements of CMI using RCF. • Experience in the use and maintenance of various lab equipment. • Knowledge of quality control methods & procedures. • Experience with cGMP and cGLP compliance standards. Show less • Co-author of a paper published in the Journal of Acquired Immune Deficiency Syndrome. • Played a key role in a multi centric Shingles Prevention study by Merck using FDA regulations and SOPs. • Experience in flow cytometry, Elispot, and cytokine analysis. • Knowledge of cell culture and tissue culture techniques. • Knowledge of cell line maintenance and cryopreservation of cells. • Experience with separation of PMNLs by histopaque techniques. • Experience in the measurements… Show more • Co-author of a paper published in the Journal of Acquired Immune Deficiency Syndrome. • Played a key role in a multi centric Shingles Prevention study by Merck using FDA regulations and SOPs. • Experience in flow cytometry, Elispot, and cytokine analysis. • Knowledge of cell culture and tissue culture techniques. • Knowledge of cell line maintenance and cryopreservation of cells. • Experience with separation of PMNLs by histopaque techniques. • Experience in the measurements of CMI using RCF. • Experience in the use and maintenance of various lab equipment. • Knowledge of quality control methods & procedures. • Experience with cGMP and cGLP compliance standards. Show less
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Amrita Institute of Medical Science & Research Center
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Kerala, India
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Microbiologist
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Jun 1998 - Feb 2001
• Experience with Automatic Blood culture system (BACTEC), Vitek, ELISA, and Western Blot. • Experience in culture and identification of various types of microorganisms. • Experience in fluorescent microscopy & fluorescent viral staining methods. • Worked on a project to check the combined effect of two antibiotics by Pfizer. • Experience in routine culture and identification of both environmental and clinically important fungi and testing anti-fungal sensitivity. • Experience with Automatic Blood culture system (BACTEC), Vitek, ELISA, and Western Blot. • Experience in culture and identification of various types of microorganisms. • Experience in fluorescent microscopy & fluorescent viral staining methods. • Worked on a project to check the combined effect of two antibiotics by Pfizer. • Experience in routine culture and identification of both environmental and clinically important fungi and testing anti-fungal sensitivity.
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Education
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Manipal Academy of Higher Education
Masters, Microbiology, General