Davide Viotto
Resercher at AB ANALITICA- Claim this Profile
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Inglese Professional working proficiency
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Italiano Native or bilingual proficiency
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Bio
Experience
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AB ANALITICA
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Italy
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Biotechnology Research
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1 - 100 Employee
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Resercher
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Oct 2021 - Present
Researcher in R&D division at AB ANALITICA company. -HERCULES, Comprehensive characterization and effective combinatorial targeting of high-grade serous ovarian cancer via single-cell analysis. European Commission, H2020-PHC-2015-two-stage. -Next Generation Sequencing nella diagnostica in-vitro oncologica. Regione Veneto, POR FESR 2014-2020, Azione 1.1.1, DGR n. 1581 del 10.10.2016 -PREDITT - Nuovi approcci predittivi per il management del tumore tiroideo. Regione Friuli Venezia Giulia, POR FESR 2014-2020, Attività 1.3.b, DGR n. 1489 del 04.08.2017 -DOMINO - Diagnostica Ospedaliera Molecolare Innovativa in NGS per l’Oncologia. Regione Friuli Venezia Giulia, POR FESR 2014-2020, Attività 1.3.b, DGR n. 1489 del 04.08.2017 -DECIDER, Improved clinical decisions via integrating multiple data levels to overcome chemotherapy resistance in high-grade serous ovarian cancer. European Commission, H2020-SC1-2020-Single-Stage-RTD -Diagnostica in vitro di biomarcatori per la medicina personalizzata in ambito oncologico basata su sequenziamento massivo parallelo di nuova generazione. Regione del Veneto POR FESR 2014-2020, Azione 1.1.4,- DGRV n. 711 del 28/05/2019
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Università Ca' Foscari Venezia
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Italy
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Higher Education
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700 & Above Employee
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ResearchER
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Oct 2020 - Sep 2021
Development of organ-on-chip, biomolecular kits for the monitoring of microplastics in agro-food products and organic matrices. Preparation of the samples to be analyzed; molecular investigation through Real Time PCR and creation of ORGAN-ON-CHIP sensors through microfluidics. Use extraction methods in collaboration with EcamRicert to be able to extract microplastics from solid and semi-solid organic matrix as agri-food products; test new biological analysis methodologies (in collaboration with Biofield Innovation Srl) to evaluate the toxicity of these contaminants directly on the foods under study; develop an organ-on-chip kit inherent to the biological field (collaboration with Biofield Innovation Srl) that gives the possibility of quantifying directly on the food the quantity of microplastics present through biochemical assays, giving to the consumers more information regarding the toxicity of the tested food.
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CRO- Centro di Ricerimento Oncologico
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Aviano, Friuli-Venezia Giulia, Italia
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Researcher
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Aug 2018 - Jun 2020
During my education and work experience I acquired technical skills in molecular biology techniques (proteins extraction from cultured cells; Western Blot analysis; RNA/DNA extractions from tissues and cells; proteins electrophoresis; immunofluorescence assay, immunoprecipitation, digital droplet PCR (ddPCR); in cellular biology techniques (culture, expansion, storage, thawing and plating of cancer and normal cell lines; DNA overexpression/silencing using cellular transfection and transduction; proliferation assay. In particular, our group in National Cancer Istitute in Aviano (Italy), is currently studying breast cancer in young women (BCYW). Compared to older patients, these women display worse response to standard therapies, higher risk of relapse and lower overall survival, especially evident in the usually less aggressive Luminal-histotype. I focused my interest on DNA library preparation for NGS sequencing. Breast cancer young women tumor tissues are used for DNA sequencing, using a homemade panel of genes chosen among the most mutated genes in luminal breast cancer. I'm experienced with IGV, Cosmic, Variant Studio data analysis softwares.
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LNCIB Area Science Park Trieste
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Trieste Area, Italy
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Biotechnologist
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Mar 2017 - Dec 2017
Studying the repression of transposable elements as a neuropathogenic mechanism in neurodegenerative diseases in genetic models of loss of Pin1. Studying the repression of transposable elements as a neuropathogenic mechanism in neurodegenerative diseases in genetic models of loss of Pin1.
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Lab internship
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May 2015 - Sep 2015
Basic microbiological techniques and cytofluerimetric assays. Evaluation of antimicrobial activity of fish peptides. Basic microbiological techniques and cytofluerimetric assays. Evaluation of antimicrobial activity of fish peptides.
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Education
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University of Trieste
Master’s Degree in Medical Biotechnologies, Medical Biotechnologies -
University of Trieste
Bachelor’s Degree in Biological Sciences and Technologies, Biological Sciences and Technologies