Experience

Autem Therapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Collaborator

  • Nov 2022 - Present



University of Alberta

• Canada
• Higher Education
• 700 & Above Employee

• Research Trainee

  • Mar 2022 - Sep 2022

  Master's Thesis project at the University of Alberta. The title of the thesis: "In vitro investigations of the effects of Tumor Treating fields and Near Infrared Photobiomodulation on cancer and normal_cells. It was a 6 month experience under the supervision of Professor J.A. Tuszynski and Professor K. Shankar as Co-supervisor. Electric fields have a considerable influence in cell functions producing a wide selection of biological effects. EF may alter functionality and perme- ability of cell membrane, resulting in a higher flow of ions leading to membrane depolarization and intracellular calcium increase concentration, both features that could trigger apoptosis. This effects have been studied for decades achiev- ing promising results in cancer treatment. The present work aims on the investigations of two different applications of EFs: a new promising therapy for cancer treatment using low-intensity (1–3 V/cm) alternating EFs with intermediate frequencies (100–500 kHz), named Tumour-Treating Fields (TTFields), observing delay in both mitosis and cy- tokinesis phases of the cell cycle and disrupting effects on the formation of the mitotic spindle during cell division leading to possible cell death. For further analysis it has been performed in vitro Cytotoxicity Assay and Cell Viability Assay which confirmed that cancer cells under TTFields treatment show higher cytotoxicity and lower viability. Next study concerns the application of Photobiomodulation (PBM) therapy using low level visible red to near infrared light (NIR) energy being absorbed by chromophores in mitochon- dria enhancing ATP production and improving cell functions. It this work it is performed turbidity measurement during tubulin polymerization in buffer solu- tion exposed to NIR light at different ranges of pulsed frequencies. Changing frequency lead to different turbidity results between exposed and unexposed tubulin samples, suggesting interesting new combinations to try investigate



Politecnico di Torino

• Research Services
• 700 & Above Employee

• Undergraduate Trainee

  • Mar 2019 - Jun 2019

  Interniship with ChiLab Laboratory Chivasso. Sviluppo di materiali polimerici compositi con proprietà funzionali e stampabili in 3D mediante stereolitografia per la realizzazione di dispositivi ChemResistor Interniship with ChiLab Laboratory Chivasso. Sviluppo di materiali polimerici compositi con proprietà funzionali e stampabili in 3D mediante stereolitografia per la realizzazione di dispositivi ChemResistor