Experience

Ludger Limited

• United Kingdom
• Biotechnology Research
• 1 - 100 Employee

• Protein/Glycan Purification scientist; Glycobiology

  • Jul 2012 - Present

  biotech company specialising in isolation/recovery/production of glycan products Protein Purification Scientist; July 2012 – Present Ludger ltd, Culham Science Centre, Abingdon, Oxfordshire Isolation and Characterisation of glycan moieties from a variety of protein sources. Various research projects to enable method design. Qc testing. • Mass spectrometry; Autoflex Speed/Maldi Tof/Tof • HPLC/UHPLC- HILIC, size exclusion, ion exchange, WAX chromatography • HPAE PAD • Electrophoresis • 2-aminobenzamidine (2-AB), 2 aminobenzoic acid (2-AA) (cyanoborohydride and Vp reductant), 9-Aminopyrene-1,4,6-trisulfonic acid (APTS) labelling and analysis • SPE • Enzyme immobilisation and glycan separation development • Monosaccharide digest and analysis • N-linked glycan release, purification and analysis • Chromeleon 6.3-7 competent and Empower 2 (to lesser degree) • Hamilton MicroLab Star Robot user • Various associated buffer production • Product QC – glycans, buffers, SPE columns, labelling kits Show less



University of Oxford

• United Kingdom
• Research Services
• 700 & Above Employee

• research assistant

  • 2008 - 2011

  Research Assistant. May 2008 – July 2011. Dept Immunology/Pathology; Sir William Dunn School of Pathology, University of Oxford. Working Project Title (s): part of SYBILLA, (systems biology of T-cell activation in health and disease). To understand the intracellular signalling network of T-cells that determines the T-cell fate. Techniques Used and Skills Acquired: siRNA/RNAi and shRNA Protein depletion HTRF assay design and validation (384well). HPLC AKTA (IEX,TiO2). Plasmid design, production, expression. Antibody development and (PCR, cloning, selection, transfection techniques) validation. Artificial signalsome expression system development. LICOR Odyssey, Flourostar Omega and Pherastar FS plate reader competent Bacterial mutation/cloning/selection Immunoblot - wet/semidry transfer SDS-PAGE/Western blot (tris/glycine, tris/tricine, agarose) Show less



University of Reading

• United Kingdom
• Higher Education
• 700 & Above Employee

• research assistant

  • Apr 2004 - Apr 2008

  Dept. of Molecular Endocrinology; School of Biological Sciences, University of Reading. Working Project Title(s) : Investigation into the N-terminal proopiomelanocortin peptides and their role in adrenal mitogenesis and steroid genesis Techniques Used and Skills Acquired:  PCR and cloning. • RNA/DNA extraction.  Receptor binding assays. • SDS-PAGE, western blot  Mass Spec sample preparation. • Immunoblotting,  Immunohistochemistry • immuno-assays, (RIA and ELISA).  Affinity purification/HPLC (AKTA) •immunoprecipitation  enzyme kinetics. •HPLC AKTA (RP, IEX)  Whole cellular assays and varied protein/peptide/DNA purification/ isolation/ quantification techniques. •IEF  Fluorescent/confocal microscopy and coulter counter work  Primary and transformed cell culture with added experience in differentiation and transfection techniques. •2D electrophoresis  In vivo Primary adrenocortical cell isolation and selection  Autonomous Undergrad and phD training and mentoring in the production of 3 undergrad dissertations and 2 phD thesi Show less



Thames Water

• United Kingdom
• Utilities
• 700 & Above Employee

• cryptosporidium analyst

  • 2001 - 2004

  Techniques Used and Skills Acquired:  Immuno-magnetic separation, immuno-fluorescent staining (FITC, DAPI).  Flow cytometry and both epi-fluorescent and direct interference contrast microscopy. Techniques Used and Skills Acquired:  Immuno-magnetic separation, immuno-fluorescent staining (FITC, DAPI).  Flow cytometry and both epi-fluorescent and direct interference contrast microscopy.