David Fraebel

Senior Scientist at Fluid-Screen
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US

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Experience

    • United States
    • Nanotechnology Research
    • 1 - 100 Employee
    • Senior Scientist
      • Sep 2022 - Present

      ● Developed experimental methods and data analysis techniques for real-time microbial detection system● Characterized two critical technical hurdles with existing method● Demonstrated feasibility of major protocol simplification to eliminate those issues while also reducing cost, labor requirements and barrier to entry for new users● Interpreted USP/PDA validation framework in the context of our technology, turned this understanding into an actionable plan, and demonstrated system’s unlocked capability for single cell detection● Coordinated actions for effective small team projects

    • Research Scientist IV
      • Aug 2021 - Sep 2022

      ● Designed and executed experimental studies to answer scientific questions critical to product development and customer applications● Leveraged findings to identify and implement data-driven improvements to products and processes● Quantified the dependence of microbial capture on fluidic, electrical, and biological variables● Optimized experimental and analysis techniques to reduce false results● Built patent pending computer vision application for bacterial quantification and robust framework for its optimization and generalization● Contributed to multiple patent applications

    • Research Scientist
      • Jul 2020 - Aug 2021

    • Higher Education
    • 1 - 100 Employee
    • Graduate Research Assistant
      • Aug 2013 - Dec 2019

      April 2014 - December 2019: Seppe Kuehn Lab (PhD project) ● Designed and executed novel experimental evolution study which used multidimensional selective pressure over hundreds of generations to reveal evolutionary trade-offs and adaptive phenotypic plasticity in E. coli ● Optimized high throughput measurements of bacterial motility and growth phenotypes and developed customized MATLAB software to extract statistical insight from large data sets ● Prepared, sequenced and analyzed hundreds of NGS libraries to characterize novel mutations ● Engineered mutant E. coli strains via point mutation and gene knockout to reveal genotype-phenotype map ● Simulated a reaction-diffusion model to connect population level adaptation to changes in single-cell traits ● Constructed custom low cost devices for automated measurement of migrating bacterial populations December 2013 - April 2014: Thomas Kuhlman Lab (Rotation) ● Quantified growth state dependent changes in gene locus motion with fluorescence microscopy and automated image segmentation August - November 2013: Paul Selvin Lab (Rotation) ● Examined motor protein activity ​in vivo with neuronal transfection and fluorescence microscopy

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Researcher
      • May 2011 - May 2013

      Mathias Lösche Lab (CMU Physics) ● Confirmed specificity of lipid-protein interaction with surface plasmon resonance real-time ​in vitro binding assay ● Prepared solid-supported lipid bilayer membranes and verified robustness with electrochemical impedance spectroscopy Mathias Lösche Lab (CMU Physics) ● Confirmed specificity of lipid-protein interaction with surface plasmon resonance real-time ​in vitro binding assay ● Prepared solid-supported lipid bilayer membranes and verified robustness with electrochemical impedance spectroscopy

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Researcher
      • May 2010 - Aug 2010

      Francisco Andrade Lab (UK Physiology) ● Constructed system for ​in vitro ​fatiguing of muscles and digital measurement of response Francisco Andrade Lab (UK Physiology) ● Constructed system for ​in vitro ​fatiguing of muscles and digital measurement of response

Education

  • University of Illinois at Urbana-Champaign
    Doctor of Philosophy - PhD, Physics
    2013 - 2019
  • Carnegie Mellon University
    Bachelor of Science - BS, Physics
    2009 - 2013

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