Experience

Kedrion Biopharma

• Pharmaceutical Manufacturing
• 700 & Above Employee

• QA Technician in Production

  • Aug 2016 - Present

  As a member of QA Operational I'm currently following production workers in their daily activities in order to ensure that Good Manifacturing Procedures (GMP) and internal Standard Operative Procedures (SOP) are correctly applied. My work includes the review of production batch records to ensure that they are compiled as required and that information therein reported corresponds to performed activities, so to grant that all archived information about each batch produced is correct and complete for future reference. Finally I'm involved in the investigation for any incident or deviation happening in the production process and in the design of corrective and preventive actions (CAPA) to avoid future incidents. Show less

• QC Technician

  • May 2012 - Aug 2016

  As a former member of the microbiology section of the QC of Kedrion, I’m an expert in “Good Manifacturing Procedures”. Also, I’ve been learning the following QC procedures, which I applied on a daily basis for more than four years in complete autonomy:Endotoxin revelation through LAL test both by Gel Clot and Endosafe methods.Microbiological sampling and analysis of industrial waters and production intermediates by direct seeding on TSA and SDA culture media, as well as filtration through Milliflex APF devices on TSA, SDA, R2A, KF and FC culture media.Sterility test of finished products through direct seeding and filtration on FTM and TSB culture media.In vivo toxicity test of finished products using mice and guinea pigs and pyrogen test using rabbits.Validation of new microbiological procedures and devices for QC purposes. Preparation of SOPs for daily use in a QC environment. Show less



Università degli Studi di Palermo

• Italy
• Higher Education
• 700 & Above Employee

• PhD

  • 2008 - 2010

  Excellent experience with Drosophila melanogaster, including: stock mantaining, sex and virgins selection, cross planning, phenotype observations by microscope, use of Flybase, larvae dissection, preparation of nuclear extracts from third instar larvae, mosaic analysis of the eye-antenna imaginal disc by double immunofluorescence, coimmunofluorescence on polythene chromosomes from salivary glands. Use and planning of FPLC. Production and purification of recombinant proteins in E. coli strains. Handling, counting, in vitro treatment, and microscope observation of human tumor cell cultures: MG63, HepG2, MDA-MB231, Y79, SK-MEL28, SAOS2, HT29, HCT116, SKHep1, Hep3B, MCF7. Transfection and gene silencing by RNAi, in vitro. Measurement of the mitochondrial transmembrane potential by JC-1 e DOC6, chromatin condensation analysis by DAPI and Hoechst, Propidium Iodide staining, measurement of cell total and free sulphidryl groups and GSH by Ellman Reagent, measurement of cell ROS by H2DCFDA, measurement of cell Ca2+ by FLUO3AM. Use of flow cytometry instruments. Study of cell cycle phases by cytofluorimetry. Immunofluorescence on in vitro cell cultures. Protein extraction and dosage from tumor cell cultures. Show less