Daria Sharko

President of the MiraCosta College Student Chapter of the ISPE at ISPE San Diego Chapter
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Contact Information
us****@****om
(386) 825-5501
Location
San Diego, California, United States, US
Languages
  • Russian Native or bilingual proficiency
  • English Professional working proficiency
  • Spanish Elementary proficiency

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Credentials

  • Annual ISPE International Emerging Leader Hackathon
    ISPE
    Oct, 2022
    - Nov, 2024
  • Lean Six Sigma Green Belt Certification
    GoLeanSixSigma.com
    Dec, 2021
    - Nov, 2024
  • Certificate of Achievement - Bioprocess Technology
    MiraCosta College
    May, 2021
    - Nov, 2024
  • Introduction to the Biology of Cancer (with Honors)
    Coursera
    Feb, 2021
    - Nov, 2024
  • Certificate of Achievement - Biomanufacturing
    MiraCosta College
    Dec, 2020
    - Nov, 2024
  • Certificate of Achievement - Biotechnology Research and Development
    MiraCosta College
    Dec, 2020
    - Nov, 2024
  • Certificate of Achievement - Laboratory Skills
    MiraCosta College
    Dec, 2020
    - Nov, 2024
  • Conflicts of Interest
    CITI Program
    May, 2022
    - Nov, 2024
  • Biomedical Research
    CITI Program
    May, 2022
    - Nov, 2024

Experience

    • United States
    • Pharmaceutical Manufacturing
    • 1 - 100 Employee
    • President of the MiraCosta College Student Chapter of the ISPE
      • Oct 2022 - Present

    • United States
    • Higher Education
    • 700 & Above Employee
    • STEM Tutor
      • Aug 2022 - Dec 2022

      Tutor of BioTech classes at the STEM Center Tutor of BioTech classes at the STEM Center

    • United States
    • Research Services
    • 700 & Above Employee
    • Student Research Intern
      • Jun 2022 - Aug 2022

      ▪ Synthesized a 5-mer N-terminal Cysteine peptide using the Solid-Phase Peptide Synthesis with a crude peptide yield of 68%. ▪ Purified the peptide by HPLC with high purity and precision confirmed by LCMS. ▪ Isolated the peptide by lyophilization for storage and future use with a final peptide yield of 45%. ▪ Effectively delivered a Journal Club presentation and a Research Project Presentation. ▪ Presented the Research Project Poster at the Scripps Symposium. ▪ Synthesized a 5-mer N-terminal Cysteine peptide using the Solid-Phase Peptide Synthesis with a crude peptide yield of 68%. ▪ Purified the peptide by HPLC with high purity and precision confirmed by LCMS. ▪ Isolated the peptide by lyophilization for storage and future use with a final peptide yield of 45%. ▪ Effectively delivered a Journal Club presentation and a Research Project Presentation. ▪ Presented the Research Project Poster at the Scripps Symposium.

    • United States
    • Biotechnology Research
    • 400 - 500 Employee
    • Manufacturing Support Technician I
      • Apr 2021 - Aug 2021

      ▪ Set high technical and ethical standards for a newly created position, connecting recombinant protein downstream and final bottling departments with monthly results that exceeded expectations by 20%. ▪ Performed 20 final bulk formulations of recombinant protein lots and bottling transfers per week. ▪ Output 30 recombinant protein lots per month including protein filtration, concentration checking, LALtesting, final gel, purity testing, labeling, and final documentation for Inventory and QC departments. ▪ Reworked recombinant protein lots to bring their specifications to desired standards (endotoxin removal, concentration adjustment). Show less

    • United States
    • Higher Education
    • 700 & Above Employee
    • Student-lab tech in the computer lab
      • Aug 2018 - May 2020

      ▪ Operated and maintained a 50 PC computer lab.▪ Assisted multiple students simultaneously with MS Office programs and PC-related issues.▪ Successfully troubleshot office equipment problems with minimal supervision.

    • Stockroom Volunteer
      • Jan 2019 - May 2019

      ▪ Calculated and prepared chemical solutions and reagents for laboratory sessions and storage.▪ Inventoried and organized lab equipment for two adjacent chemical laboratories.▪ Washed a wide variety of chemical glassware.

Education

  • MiraCosta College
    Bachelor of Science - BS, Biomanufacturing
    2021 - 2023
  • GoLeanSixSigma.com
    Lean Six Sigma Green Belt
    2021 - 2021
  • MiraCosta College
    Associate of Science - AS, Biomanufacturing, R&D
    2018 - 2020
  • MiraCosta College
    Associate of Arts - AA, Mathematics and Sciences
    2018 - 2020

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