Daria Sharko
President of the MiraCosta College Student Chapter of the ISPE at ISPE San Diego Chapter- Claim this Profile
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Russian Native or bilingual proficiency
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English Professional working proficiency
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Spanish Elementary proficiency
Topline Score
Bio
Credentials
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Annual ISPE International Emerging Leader Hackathon
ISPEOct, 2022- Nov, 2024 -
Lean Six Sigma Green Belt Certification
GoLeanSixSigma.comDec, 2021- Nov, 2024 -
Certificate of Achievement - Bioprocess Technology
MiraCosta CollegeMay, 2021- Nov, 2024 -
Introduction to the Biology of Cancer (with Honors)
CourseraFeb, 2021- Nov, 2024 -
Certificate of Achievement - Biomanufacturing
MiraCosta CollegeDec, 2020- Nov, 2024 -
Certificate of Achievement - Biotechnology Research and Development
MiraCosta CollegeDec, 2020- Nov, 2024 -
Certificate of Achievement - Laboratory Skills
MiraCosta CollegeDec, 2020- Nov, 2024 -
Conflicts of Interest
CITI ProgramMay, 2022- Nov, 2024 -
Biomedical Research
CITI ProgramMay, 2022- Nov, 2024
Experience
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ISPE San Diego Chapter
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United States
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Pharmaceutical Manufacturing
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1 - 100 Employee
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President of the MiraCosta College Student Chapter of the ISPE
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Oct 2022 - Present
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MiraCosta College
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United States
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Higher Education
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700 & Above Employee
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STEM Tutor
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Aug 2022 - Dec 2022
Tutor of BioTech classes at the STEM Center Tutor of BioTech classes at the STEM Center
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Scripps Research
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United States
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Research Services
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700 & Above Employee
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Student Research Intern
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Jun 2022 - Aug 2022
▪ Synthesized a 5-mer N-terminal Cysteine peptide using the Solid-Phase Peptide Synthesis with a crude peptide yield of 68%. ▪ Purified the peptide by HPLC with high purity and precision confirmed by LCMS. ▪ Isolated the peptide by lyophilization for storage and future use with a final peptide yield of 45%. ▪ Effectively delivered a Journal Club presentation and a Research Project Presentation. ▪ Presented the Research Project Poster at the Scripps Symposium. ▪ Synthesized a 5-mer N-terminal Cysteine peptide using the Solid-Phase Peptide Synthesis with a crude peptide yield of 68%. ▪ Purified the peptide by HPLC with high purity and precision confirmed by LCMS. ▪ Isolated the peptide by lyophilization for storage and future use with a final peptide yield of 45%. ▪ Effectively delivered a Journal Club presentation and a Research Project Presentation. ▪ Presented the Research Project Poster at the Scripps Symposium.
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BioLegend
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United States
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Biotechnology Research
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400 - 500 Employee
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Manufacturing Support Technician I
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Apr 2021 - Aug 2021
▪ Set high technical and ethical standards for a newly created position, connecting recombinant protein downstream and final bottling departments with monthly results that exceeded expectations by 20%. ▪ Performed 20 final bulk formulations of recombinant protein lots and bottling transfers per week. ▪ Output 30 recombinant protein lots per month including protein filtration, concentration checking, LALtesting, final gel, purity testing, labeling, and final documentation for Inventory and QC departments. ▪ Reworked recombinant protein lots to bring their specifications to desired standards (endotoxin removal, concentration adjustment). Show less
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MiraCosta College
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United States
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Higher Education
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700 & Above Employee
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Student-lab tech in the computer lab
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Aug 2018 - May 2020
▪ Operated and maintained a 50 PC computer lab.▪ Assisted multiple students simultaneously with MS Office programs and PC-related issues.▪ Successfully troubleshot office equipment problems with minimal supervision.
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Stockroom Volunteer
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Jan 2019 - May 2019
▪ Calculated and prepared chemical solutions and reagents for laboratory sessions and storage.▪ Inventoried and organized lab equipment for two adjacent chemical laboratories.▪ Washed a wide variety of chemical glassware.
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Education
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MiraCosta College
Bachelor of Science - BS, Biomanufacturing -
GoLeanSixSigma.com
Lean Six Sigma Green Belt -
MiraCosta College
Associate of Science - AS, Biomanufacturing, R&D -
MiraCosta College
Associate of Arts - AA, Mathematics and Sciences