Daniel Nguyen
Scientist II at Editas Medicine- Claim this Profile
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Bio
Experience
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Editas Medicine
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United States
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Biotechnology Research
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100 - 200 Employee
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Scientist II
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Feb 2022 - Present
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Associate Scientist II
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Oct 2020 - Feb 2022
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Associate Scientist I
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Jun 2019 - Oct 2020
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Compass Therapeutics LLC
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United States
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Mental Health Care
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1 - 100 Employee
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Principal Associate Scientist
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Oct 2018 - Apr 2019
Cambridge, Massachusetts • Provided cell sort troubleshooting and optimization for mammalian display selection and B cell cloning for Antibody Discovery and Engineering team. • Performed MACS, FACS (analysis and sorting), 10X genomics, cell culture, plasmid cloning, molecular biology. • Collaborated across departments with sorting projects and stable cell line development. • Operation and maintenance of Aria III and Fusion sorter instruments.
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The Forsyth Institute
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United States
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Research Services
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100 - 200 Employee
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Flow Cytometry / Cell Sorter Core Manager
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Mar 2014 - Oct 2018
• Manage core scheduling and billing responsibilities. Establish new working accounts with internal and external researchers for use of core facility. • Maintain BD FACS Aria II cell sorter equipment and perform routine instrument calibration for optimized performance. • Maintain Invitrogen™ Attune™ NxT flow cytometer equipment and perform routine instrument calibration for optimized performance. • Set up, optimize and run fluorescent-activated cell sorting (FACS)… Show more • Manage core scheduling and billing responsibilities. Establish new working accounts with internal and external researchers for use of core facility. • Maintain BD FACS Aria II cell sorter equipment and perform routine instrument calibration for optimized performance. • Maintain Invitrogen™ Attune™ NxT flow cytometer equipment and perform routine instrument calibration for optimized performance. • Set up, optimize and run fluorescent-activated cell sorting (FACS) experiments as well as cell population analysis experiments. • Provide consultation for researchers with questions related to flow cytometry and experimental design, including multi-color/multi-parametric analysis. • Train researchers on principals of fluorescence, detection and flow cytometry application. Train researchers how to operate cytometer and software functions. • Communicate with cytometer manufacturers reporting bugs, issues, or new feature requests. • Arrange seminars at Forsyth to educate researchers on new and current flow applications, technologies, and data analysis techniques. • Collaborate with flow cytometry related online services to enhance researcher productivity.
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Senior Research Associate
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Oct 2010 - Oct 2018
Department of Applied Oral Sciences • Investigate periodontal disease pathways, progression, and resolution. Bridging the gap between science and therapeutics through translational research. Routinely analyze clinical samples, cell signaling pathways, gene expression, DNA-DNA hybridization, perform immunoassays, process and analyze histology tissue samples, microbiology culture, and in vitro cell culture. • Manage laboratory practices, standard operating procedures, equipment operation training, maintain equipment… Show more • Investigate periodontal disease pathways, progression, and resolution. Bridging the gap between science and therapeutics through translational research. Routinely analyze clinical samples, cell signaling pathways, gene expression, DNA-DNA hybridization, perform immunoassays, process and analyze histology tissue samples, microbiology culture, and in vitro cell culture. • Manage laboratory practices, standard operating procedures, equipment operation training, maintain equipment servicing and calibration, etc. • Lab supply management and project reagent/material sourcing. • Lab safety representative, waste flow management, emergency preparedness, and emergency response. CPR certified. • Training of visiting scientists, clinicians, students, and research scholars on safe lab practices and experimental protocols. • Collaborate with external and internal scientists in sample analysis, training methodologies, and consultation. • Supervise lab operations, experiments, and provide assistance/consultations to researchers. • Collaborate with equipment manufacturers and lab supply vendors in testing new methods/products for laboratory research. • Presenter at American/International Association for Dental Research Conference (2010, 2012, 2013, 2014 and 2015) • Co-Author of "ERV1 Overexpression in Myeloid Cells Protects against High Fat Diet Induced Obesity and Glucose Intolerance." Sci Rep. 2017 • Co-Author of "Strain-Specific Impact of Fusobacterium Nucleatum on Neutrophil Function." J Periodontol. 2017 • Co-Author of "Dental plaque microbial profiles of children from Khartoum, Sudan, with congenital heart defects." J Oral Microbiol. 2017 • Co-Author of "Endothelial Cell response to Fusobacterium nucleatum." Infect Immun. 2016 • Co-Author of "Maresin 1 Biosynthesis and Pro-resolving Anti-Infective Functions with Human Localized Aggressive Periodontitis Leukocytes." Infect Immun. 2015
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Education
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Marine Biological Laboratory
Certificate, Immunohistochemistry and Microscopy Course -
Massachusetts College of Pharmacy and Health Sciences
Bachelor of Science (B.S.), Pre-Medicine/Pre-Medical Studies