Daniel Loftus

PHD Candidate at Harvard University
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Contact Information
us****@****om
(386) 825-5501
Location
Greater Boston

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Experience

    • United States
    • Higher Education
    • 700 & Above Employee
    • PHD Candidate
      • Sep 2018 - Present

      Graduate research on the role of allele-specific chromatin structure in genomic imprinting in the brain.• Discovered a role for pre-existing allelic chromatin structure in genomic imprinting in the brain. • Designed and executed an allele-specific region capture Hi-C protocol, including cell culture, library prep, and bioinformatics. • Performed bioinformatics analysis of both self-generated and publicly available NGS datasets including Hi-C, RNA-seq, ChIP-seq, ATAC-seq, DNase-seq, and PRO-seq using bash, Slurm, and python. • Leveraged genetic and epigenetic modifiers (lentivirus, transposases, Cas9, dCas9) in an in vitro neuron differentiation system to characterize allele-specific gene regulation.• Mentored multiple lab members, including 4 rotating graduate students and 4 undergraduates. Show less

    • Teaching Assistant
      • Aug 2019 - Dec 2019

      • Led a discussion section, lab, and office hours for 16 undergraduate students in introductory molecular biology and biochemistry. • Graded problem sets, lab reports, and papers.

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Researcher
      • Oct 2016 - May 2018

      • Conducted multiple independent research projects involving yeast large ribosomal subunit biogenesis. • Designed and executed site-directed and random mutagenesis screens of ribosome biogenesis factors. • Conducted multiple independent research projects involving yeast large ribosomal subunit biogenesis. • Designed and executed site-directed and random mutagenesis screens of ribosome biogenesis factors.

    • United States
    • Education Administration Programs
    • 1 - 100 Employee
    • Academic Tutor
      • May 2015 - May 2018

      Tutored over 150 middle school, high school, and college students in subjects ranging from mathematics, biology, chemistry, physics, Spanish, history, and test prep. Tutored over 150 middle school, high school, and college students in subjects ranging from mathematics, biology, chemistry, physics, Spanish, history, and test prep.

    • United States
    • Museums, Historical Sites, and Zoos
    • 700 & Above Employee
    • Laboratory Assistant
      • Jun 2016 - Aug 2016

      • Performed DNA extraction, PCR, and sequencing of feline scat samples. • Assisted with population genetics analysis for South American puma conservation work. • Trained 2 new lab assistants in lab techniques and protocols. • Performed DNA extraction, PCR, and sequencing of feline scat samples. • Assisted with population genetics analysis for South American puma conservation work. • Trained 2 new lab assistants in lab techniques and protocols.

Education

  • Harvard University
    2018 - 2023
  • The University of Texas at Austin
    Bachelor of Science - BS, Biochemistry and Molecular Biology

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