Daniel de Castro Assumpção
PhD Student at Northwestern University- Claim this Profile
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Portuguese Native or bilingual proficiency
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English Full professional proficiency
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Spanish Full professional proficiency
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Italian Full professional proficiency
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French Limited working proficiency
Topline Score
Bio
Experience
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Northwestern University
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United States
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Higher Education
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700 & Above Employee
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PhD Student
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Sep 2021 - Present
Chemical and Biological Engineering Chemical and Biological Engineering
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Amyris
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United States
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Biotechnology Research
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700 & Above Employee
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Senior Associate Scientist - Fermentation
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Nov 2020 - Jul 2021
Designed and analyzed fermentation experiments to maximize understanding and performance of strains and meet goals of yield, productivity and purity for two projects. Worked closely with: operations team to plan and execute the experiments effectively; with biologists to understand the impact of genetic changes and test the strains fairly; with downstream team to deliver representative material for separations and purification processes development, generating samples for partnership milestones.
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BioProcess Development Supervisor
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Jul 2020 - Oct 2020
Supervised all Pilot Plant fermentation operations, leading a group of 3 research associates. Responsible for planning fermentation operations, sugar feedstock production, maintenance, and often operating the bioreactors. Created and formalized bioreactor decontamination protocol.• Created and formalized an contamination response protocol for fermentations.• Managed sugar feedstock production schedule and operations.
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BioProcess Development Associate III
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Sep 2019 - Jun 2020
Planned, performed and analyzed the results of pilot scale fermentations of several key company projects. Responsible for a big effort in operations documentation with 2 main goals achieved: (1) Lead the task force to write +100 SOPs for the plant operation; and (2) authored MFCS (Multi Fermentor Control System) manual for the company, facilitating operations in several departments.
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Codexis, Inc.
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United States
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Biotechnology Research
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100 - 200 Employee
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Process Engineer
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Mar 2018 - Sep 2019
• Fermentative process development (upstream and downstream) for different industrial and biotherapeutics enzymes production. • Use of scaledown models (plugflow reactors) to create anoxic zones and regions of high substrate concentration to predict fermentation processes at different industrial scale.. Tech transfer the process to large scales, compare to scale down model and suggest improvements on predictive experimental models. • Experienced with downstream process for enzyme recovery: homogenization, cell debris flocculation and centrifugation, ultrafiltration, diafiltration, sterilefiltration and lyophilization. • In case of product off specification, searched in literature for strategies to improve the identified issue and applied it (troubleshooting). • Very organized employee, responsible for maintenance of 12 tanks + downstream equipment, activity scheduling, CAPEX and OPEX estimation, documentation (Process Safety Reviews, SOP, Batch Records, and laboratory organization. Show less
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Universidade Estadual de Campinas
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Brazil
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Research Services
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700 & Above Employee
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Masters Student
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Jul 2015 - Jul 2017
R&D on fermentation processes. Industrial flexibilization of n-butanol and ethanol production from lignocellulosic biomass. Skills: • Biomass chemical characterization • Biomass pretreatment and enzymatic hydrolysis • Laboratory experience on fermentation process (bacterial and yeast fermentation) • High performance liquid chromatography (HPLC) • Design of Experiment (DoE) and surface response methodology (SRM) expertise. • Technic-economic analysis of bio refineries (mentored 3 undergraduate projects on this area) • Teaching Assistant (thermodynamics, heat transfer phenomena and Technic-economic analysis) Show less
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The Ohio State University
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United States
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Higher Education
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700 & Above Employee
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Invited Researcher
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Mar 2017 - Apr 2017
Department of Animal Sciences, under supervision of Prof. Thaddeus Ezeji. Bacterial fermentation (Clostridium sp.) to Acetone, n-Butanol and Ethanol production from hydrolyzed eucalyptus. Skills: • Anaerobic fermentation • Fermentation broth detoxification strategies • Gas Chromatography and (GC) High Performance Liquid Chromatography (HPLC) Department of Animal Sciences, under supervision of Prof. Thaddeus Ezeji. Bacterial fermentation (Clostridium sp.) to Acetone, n-Butanol and Ethanol production from hydrolyzed eucalyptus. Skills: • Anaerobic fermentation • Fermentation broth detoxification strategies • Gas Chromatography and (GC) High Performance Liquid Chromatography (HPLC)
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Mossi Ghisolfi Group
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Chemical Manufacturing
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1 - 100 Employee
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R&D Process Engineer
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Dec 2012 - Jul 2015
• R&D on the second generation ethanol fermentation process using lignocellulosic raw material as a substrate. • Commissioning and Start Up of a second generation ethanol facility in Alagoas, Brazil. • Technic-Economic assessment of the integration between first and second generation ethanol production facilities in Brazil and Argentina. • R&D on the second generation ethanol fermentation process using lignocellulosic raw material as a substrate. • Commissioning and Start Up of a second generation ethanol facility in Alagoas, Brazil. • Technic-Economic assessment of the integration between first and second generation ethanol production facilities in Brazil and Argentina.
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Raízen
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Renewable Energy Power Generation
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700 & Above Employee
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Project Engineering Intern
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Feb 2011 - Jul 2011
Intern at project engineer department. Adequate process diagrams (PFD and P&ID) to the ANSI ISA 5.1. Intern at project engineer department. Adequate process diagrams (PFD and P&ID) to the ANSI ISA 5.1.
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Education
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Northwestern University
Doctor of Philosophy - PhD, Chemical and Biological Engineering -
University of Campinas
Masters of Science - MS, Chemical Engineering -
University of Campinas
Bachelor of Science - BS, Chemical Engineering -
Universidad Nacional de Río Cuarto
None - Study Abroad, Chemical Engineering