Experience

Pearl Diagnostics, Inc.

• United States
• Medical Equipment Manufacturing
• 1 - 100 Employee

• Research And Development Scientist

  • Jul 2022 - Present

  Develop early detection diagnostic products for fungal infections. Develop early detection diagnostic products for fungal infections.



Novel Microdevices

• United States
• Biotechnology Research
• 1 - 100 Employee

• Product Research Scientist

  • Sep 2021 - Jun 2022

  - Design and validate new IVD multiplex molecular point of care diagnostics - Independent experimental planning, design, hands-on execution, analysis, reporting, and documenting per GLP and QMS of multiplex molecular assays - Actively participate in defining project activities and timelines to achieve project goals in a timely manner - Ability to work in a results-driven R&D environment developing multiple products - Design and validate new IVD multiplex molecular point of care diagnostics - Independent experimental planning, design, hands-on execution, analysis, reporting, and documenting per GLP and QMS of multiplex molecular assays - Actively participate in defining project activities and timelines to achieve project goals in a timely manner - Ability to work in a results-driven R&D environment developing multiple products



The Janssen Pharmaceutical Companies of Johnson & Johnson

• United States
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Scientist

  • Aug 2019 - Oct 2021

  - Drug product development for cancer immunotherapy vaccine. Leads research and designs protocols. Culture viral specimens of BSL-2 virus, Herpes Simplex Virus-1 for infectivity assays. - Optimize and validate cellular and molecular assays for quality assurance; tissue culture, qPCR, plaque assays. - Compiles and interprets data. Present results with diverse team, effectively communicate and collaborate to reach goals. - Develop and review Standard Operating Protocols. - Formulate vaccines for mouse studies. Show less



National Institute of Allergy and Infectious Diseases (NIAID)

• United States
• Government Administration
• 700 & Above Employee

• Biologist

  • Sep 2018 - Aug 2019

  -Performs range of procedures for BSL2 influenza research, such as PBMC isolation from patient samples, virus titration and purification. -Optimize PCR for a dozen influenza strains as well as cloning and transformation procedures. -Design and implement SOPs for all experiments involved in plasmid transformation. -Performs range of procedures for BSL2 influenza research, such as PBMC isolation from patient samples, virus titration and purification. -Optimize PCR for a dozen influenza strains as well as cloning and transformation procedures. -Design and implement SOPs for all experiments involved in plasmid transformation.



Ecovative Design

• India
• Design Services
• 1 - 100 Employee

• Research Biologist

  • Mar 2013 - Sep 2018

  - Develop molecular assays to determine antibiotic production from a strain of streptomycetes on agar plates. - Adapt HPLC method to isolate specific proteins from liquid cultures. - Cloning bacteria using Gibson Assembly and intergeneric conjugation by E.coli donors. Plasmid purification and isolation. - DNA purification from gels, PCR and designing primers. - Manage R&D supplies; monitor consumable supplies, place purchase orders and maintain the stock supply. - Established and conducts R&D meetings to promote collaboration among projects and updates on advancements - Supports the development of Standard Practice Committee, created to standardize protocols across the company. - Ensure calibration of equipment, such as laminar flow hoods, chemical hood, pipettors,etc. - Perform environmental monitoring of R&D work spaces to ensure low bioburden. Became familiar with and can identify common soil ascomycetes contaminates. Show less



Johns Hopkins School of Medicine

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Technologist

  • Oct 2010 - Mar 2013

  • Managed thousands of DNA samples used for a large scale study. Organizes all the samples and runs them on the Hamilton Microlab StarLet robot for genotyping and locating a specific germline mutation for prostate cancer. • Updated and maintained large database with all used samples on Microsoft Access and Excel. • Worked toward developing an ELISA by using patient blood serum and potential biomarkers to test for early detection. Needed to isolate and purify proteins from cells and serum. • Conducted siRNA transfection and soft agar assay with cancer cell lines to observe tumorigenesis with and without the expression of specific genes. • Executed lab maintenance when needed, sterilized Pasteur pipette tips, cleaned and filled water baths, ensured buffers were prepared. Show less



BioTechnical Institute of Maryland, Inc.- BioSciConcepts

• Workshop Participant

  • Sep 2011 - Oct 2011

  • Participated in the Recombinant DNA, PCR and RT-PCR workshops. • In the workshops I used various techniques to accomplish goals such as subcloning using DNA that I isolated from DNA agarose gels, the performed ligation and transformed competent E.coli which was analyzed by blue-white screening. • Studied process of amplification for PCR, how to troubleshoot experimental conditions and make primers. • Used different samples to study RT-PCR and gene expression by developing templates and analyzing results. Show less



Towson University Science Department

• United States
• Higher Education
• 1 - 100 Employee

• Student

  • Jan 2010 - May 2010

  • Semester long project to subclone the lux operon into an E.coli plasmid. • Semester long project to subclone the lux operon into an E.coli plasmid.



suffolk County DPW

• Facilities Services
• 1 - 100 Employee

• Summer Intern

  • Jun 2008 - Aug 2008

  -Assisted in monitoring over 200 Suffolk County beaches. Documenting all characteristics that could affect the quality of the water. -Collected water samples to be tested for cyanobacteria. -Maintained notebook of all details documented for future reference and comparing. -Assisted in monitoring over 200 Suffolk County beaches. Documenting all characteristics that could affect the quality of the water. -Collected water samples to be tested for cyanobacteria. -Maintained notebook of all details documented for future reference and comparing.