Experience

STEM Ambassadors

• United Kingdom
• Education Administration Programs
• 1 - 100 Employee

• STEM Ambassador

  • Jun 2020 - Present



King's College London

• United Kingdom
• Research Services
• 700 & Above Employee

• PHD Student

  • Jan 2018 - Present

  Development and validation of a biologically relevant, 3D-dynamic model of liver fibrosis.The cross-talk between parenchymal cells, the extracellular matrix (ECM) and the innate immune system plays an important role in modulating liver homeostasis and driving disease. Fibrosis is a hallmark of liver disease and is characterised by an accumulation of ECM proteins secreted by activated Hepatic Stellate Cells (HSCs). Fibrosis, however, is reversible and if the underlying injury is eliminated the liver is able to revert from a fibrotic towards a more healthy state. A better understanding of the cross talk events underlying the development and the reversal of fibrosis could lead to the identification of novel therapeutic targets to treat or prevent liver diseases, however, in order to explore the complex cross-talk events behind fibrosis we need a disease model that incorporates the parenchymal cells, ECM and the circulating immune system.The aim of my PhD project is to develop a humanised and physiologically relevant model of liver fibrosis which incorporates the extracellular matrix, parenchymal cells and a circulating immune component.



The Brilliant Club

• United Kingdom
• Higher Education
• 100 - 200 Employee

• Academic Tutor

  • Jul 2020 - Dec 2020



THE FOUNDATION FOR LIVER RESEARCH

• Research Technician. Laboratory Luca Urbani

  • Sep 2017 - Jan 2018



UPMC

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Technician. Laboratory: Harry SOKOL

  • Jun 2016 - Jul 2017

  Research area: The role of the intestinal microbiota in Inflammatory Bowel Diseases (IBD)Description: Production and optimisation of a cell culture model of IBD. Routine DNA extractions and genotyping. Presenting and communication in french.



INSERM

• France
• Research Services
• 700 & Above Employee

• Research Technician. Laboratory: Philippe SEKSIK

  • Aug 2014 - Feb 2016

  General day-to-day running of the lab as well as carrying out work for PhD and Post-doc projects. My research focussed on two areas: firstly, the interplay between the gut microbiota and the host immune system, particularly in the context of IBD and Chrons Disease; and secondly on the regulation of Endoplasmic reticulum stress in the context of inflammation.



UPMC

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research assistant (Collaboration with Dominique RAINTEAU)

  • May 2015 - Jun 2015

  Research area: Bile acid metabolism and associated diseases.Description: Preparation of bile acid samples by SPE column and acetonitrile extraction for bile acid profiling on HPLC-Mass Spec. This work contributed to a publication.



University of Glasgow

• United Kingdom
• Higher Education
• 700 & Above Employee

• Research Masters (Mres)

  • Aug 2012 - Aug 2014

  Title: Characterisation of two key virulence factors in Escherichia coli O157:H7.Abstract:Enterohaemorrhagic E. coli (EHEC) are a subset of pathogenic E. coli which can cause diarrhoeal disease, with the majority of infections due to serovar O157:H7. Successful infection by EHEC is determined by the expression of two key virulence factors, flagella and the type three secretion system (T3SS), a bacterially encoded needle-like filament. Flagella are responsible for directional and targeted swimming of the bacteria as well as for initiating bacteria: host cell attachment. A subsequent switch to T3SS expression promotes enhanced binding via the transmission of bacterial proteins (termed effectors) into the host cell. Once in the host cell these effectors act to manipulate host cell pathways and further aid infection. This thesis sets out to explore these two important virulence factors; firstly by assessing the potential of a novel fluorescent reporter molecule, LOV, in imaging the expression, translocation and host cell localisation of T3SS injected effector proteins; and secondly by exploring the regulation of flagella rotation, and thus motility, in a series of Acetaldehyde coA dehydrogenase (AdhE) deletion mutants.Findings from this study show that the LOV domain can be used to fluorescently tag the bacterial effector, Tir, and monitor it’s expression, translocation through the T3SS and localisation within the host cell, in real-time. This opens up exciting new possibilities in using LOV to fluorescently monitor the localisation of bacterial effector proteins within the host cell and thus give information on potential cellular partners and mechanism of action. Additionally, exploration of flagella rotation regulation, through the isolation and genomic sequencing of a set of AdhE deletion mutants, suggested a role for AdhE in bacterial motility via indirect acetylation of the chemotaxis protein CheY.



Institut Pasteur

• France
• Research Services
• 700 & Above Employee

• Student Intern

  • Jun 2013 - Dec 2013



The University of Edinburgh

• United Kingdom
• Research Services
• 700 & Above Employee

• Research assistant

  • Jun 2010 - Sep 2010