Experience

Advanced Cell Diagnostics

• United States
• Biotechnology Research
• 100 - 200 Employee

• Field Application Scientist

  • Feb 2021 - Present

  Field Application Scientist (FAS) for RNAscope, BaseScope, DNAscope and HiPlex assays in the Boston/Cambridge area. Education and training of researchers at the bench as well as online. Installation and training to run experiments on Leica BOND RX and Ventana ULTRA automated slide stainers. Troubleshooting and optimization of difficult protocols combining immunostaining or immunofluorescence with the RNAscope, DNAscope and other Advanced Cell Diagnostics technologies. Field Application Scientist (FAS) for RNAscope, BaseScope, DNAscope and HiPlex assays in the Boston/Cambridge area. Education and training of researchers at the bench as well as online. Installation and training to run experiments on Leica BOND RX and Ventana ULTRA automated slide stainers. Troubleshooting and optimization of difficult protocols combining immunostaining or immunofluorescence with the RNAscope, DNAscope and other Advanced Cell Diagnostics technologies.



Massachusetts Institute of Technology

• United States
• Higher Education
• 700 & Above Employee

• Research Scientist, Neuroscience - Immunostaining & Imaging

  • Aug 2016 - Dec 2020

  o Developed histological methods to study large scale events in the brains relating to Parkinson’s disease, Huntington’s disease, OCD, autism, emotion and decision making. o Did perform and coordinate the immunohistochemical and other staining procedures as well as light, fluorescence, confocal microscope and slide scanner imaging of whole tissue sections. o Labelling of the striatum and substantia nigra with specific markers for the striosome and matrix compartments, the d1r and the d2r medium spiny neurons (MSNs). o Supervision of the lab work of Undergraduate Research Opportunities Program Students (UROPs). o Worked closely with the team to generate progress reports and track budgets. Show less



Harvard Medical School

• United States
• Higher Education
• 700 & Above Employee

• Harvard Instructor in Neurology and Lab Manager: Immunohistology, Microscopy & Image Analysis

  • Jan 2013 - Aug 2016

  o Principal Researcher in a collaboration with Biogen Idec examining the meninges and the choroid plexus from diseased and healthy human archival brains for the presence of JC Virus (JCV).o Discovery of JCV-infected cells in the meninges and choroid plexus of a fatal case of JCV meningitis.o Successfully developed and optimized two to five colors multiplex immunohistochemistry and immunofluorescence to simultaneously examine JCV proteins, brain cells phenotypes, MHC Class I expression and immune cells.o Collaborated with Multiple Sclerosis (MS) specialists from the Mayo Clinic at Rochester in a research on the brain of a unique, atypical patient who died of both Progressive Multifocal Leukoencephalopathy (PML) and MS. Successfully characterized myelin proteins losses and inflammatory infiltrates in separate and combined PML and MS lesions.o Evaluation and validation of customs JCV Agno protein and T antigen antibodies for use on human archival brains. Show less

• Instructor in Neurology and Manager: Microscopy, Image Analysis & Laser Capture Microdissection

  • Jan 2006 - Dec 2012

  o Principal researcher in a collaboration with Biogen Idec to determine if there was a reactivation of SV40 in the brains, kidneys, lungs, lymph nodes, pancreas and Thymus of monkeys treated with the drug Natalizumab (Tysabri).o Developed and optimized multiplex immunolabeling of JC Virus, brain cells and disease markers leading to the discovery of JCV Encephalopathy (JCVE).o Successful single cell laser capture microdissection, viral DNA extraction and clone identification of different brain cells types (neurons, oligodendrocytes, astrocytes) stained by IHC. o Supervision of 4 Research Assistants. Show less

• Senior Postdoctoral Fellow

  • Jan 2003 - Dec 2005

  o Developing and standardizing immunohistochemical methods for the detection and characterization of JC virus. This work also led to the discovery of JCV granule cell neuronopathy (JCV GCN). o Principal Researcher in an IHC study done for the US Department of Justice to see in SV40 was present in brain cancer biopsies.o Supervision of 1 Research Assistant.

• Roche Research Fellow

  • Feb 2002 - Jan 2003

  o Responsible for developing a co-localization analysis of immune T cells with JC Virus infected cells and PML lesions on whole slide panoramic dual labeled immunohistochemical images.o Set up and managed a histology lab, including immunohistochemistry, microscopy, and image analysis.



University of Lausanne - UNIL

• Switzerland
• Research Services
• 700 & Above Employee

• Senior Instructor

  • 2000 - 2001

  o Supervision of the Instructors in plant morphology and histology.

• Instructor

  • 1995 - 2000

  o Teaching and supervision of practical works in plant physiology (winter semester) and plant morphology and histology (summer).o Mentor for graduate students in Biology.



Swiss Scientific Research Center - Abidjan (Ivory Coast)

• Abidjan, Yéalé and Thai National Park (Ivory Coast)

• Graduate Research Fellow

  • Feb 1993 - Jun 1994

  o Research on the Micropotamogale lamottei, an endangered species of the rainforests of the Mount Nimba Reserve between Ivory Coast, Guinea and Liberia. o Radio-tracking, trapping, breeding, dissecting and karyotyping of regional micro-mammals. o Research on the Micropotamogale lamottei, an endangered species of the rainforests of the Mount Nimba Reserve between Ivory Coast, Guinea and Liberia. o Radio-tracking, trapping, breeding, dissecting and karyotyping of regional micro-mammals.



Swiss Federal Agricultural Research Station - Agroscope

• Research Fellow.

  • 1992 - 1993

  Light trapping, sexual pheromones trapping, sexual confusion, sexual flights, breeding.

• Research Fellow

  • 1991 - 1992

  Rodenticides toxicity tests, dissections, development of strategies minimizing secondary poisoning.



Ecole Catholique du Valentin. Lausanne. Switzerland

• Lausanne. Switzerland.

• Science Teacher

  • 1990 - 1991

  o Sciences teacher. o Sciences teacher.



ETAT DE VAUD Département de la formation, de la jeunesse et de la culture

• Lausanne Area, Switzerland

• Substitute Teacher

  • 1983 - 1990

  Substitute Teacher of the State of Vaud (Switzerland) Sports, mathematics, sciences, industrial art, French, English, German. Substitute Teacher of the State of Vaud (Switzerland) Sports, mathematics, sciences, industrial art, French, English, German.



Swiss Rowing Team

• Lausanne, Neuchatel, Schiffenen & Sarnen

• Rower (Swiss Rowing Team)

  • 1982 - Jul 1987

  Swiss Champion (Elite) in coxed and coxless fours in 1982 & 1986. Selected to the U23 World Championships (Match des Seniors) in Vienna (1982) & Banyoles (1985). Selected to World Cup Regattas (Lucerne, Vichy, Mannheim, Duisburg, Essen). Swiss Champion (Elite) in coxed and coxless fours in 1982 & 1986. Selected to the U23 World Championships (Match des Seniors) in Vienna (1982) & Banyoles (1985). Selected to World Cup Regattas (Lucerne, Vichy, Mannheim, Duisburg, Essen).