Experience

Septerna

• United States
• Biotechnology
• 1 - 100 Employee

• Scientist

  • Sep 2022 - Present

• Associate Scientist

  • Dec 2021 - Sep 2022



Theravance Biopharma US, Inc.

• United States
• Biotechnology
• 100 - 200 Employee

• Associate Scientist

  • Feb 2021 - Nov 2021

  Utilize HighRes Biosolutions’ integrated automation system and Cellario scheduling software to perform fully automated cellular screens and to adapt manual assays onto the automated system Develop a reproducible and sensitive proliferation assay using IncuCyte® live cell imager and nuclear staining reagent NucLight Red to determine compounds’ effect on cell lines of interest Investigate the effects of efflux transporters on compound potency in human versus rodent primary cell… Show more Utilize HighRes Biosolutions’ integrated automation system and Cellario scheduling software to perform fully automated cellular screens and to adapt manual assays onto the automated system Develop a reproducible and sensitive proliferation assay using IncuCyte® live cell imager and nuclear staining reagent NucLight Red to determine compounds’ effect on cell lines of interest Investigate the effects of efflux transporters on compound potency in human versus rodent primary cell lines Supervise direct report to perform cell cytotoxicity and biochemical screens on Company’s novel compounds

• Senior Research Associate

  • Feb 2019 - Feb 2021

  Operated liquid handlers (Hamilton STARlet, Labcyte Echo, Agilent Bravo) and integrated robotics to perform cellular screens using AlphaLISA as the final readout. Provided training for colleagues who want to integrate liquid handler and robotics into their workflow. Utilized Promega’s NanoBRET technology to investigate target engagement and compound kinetics. Completed training provided by Hamilton for VENUS software used by Hamilton’s STAR/STARlet.

• Research Associate II

  • Oct 2017 - Feb 2019

  Tested for target engagement of compounds in tissue homogenates through quantification of target protein using Protein Simple’s Western Blot System (WES). Developed in-house target engagement screening for various tissues with ECL technology (MSD) to be used in PK/PD analysis. Performed cytotoxicity screen using Cell-Titer Glo on Company’s small molecule compounds.



BioCheck, Inc.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Associate

  • Sep 2016 - Oct 2017

  Participated in the product verification and validation of specialized ELISA assays that will act as a gold standard for brain trauma detection Performed in-process QC and generate performance characteristics data for various sandwich-ELISA assay kits under CGMP environment Assisted with assay development, optimization, and validation of traditional ELISA assays and single molecule immunoassays (Quanterix SimoaTM) Drafted IQ/OQ documentation for routine calibration of… Show more Participated in the product verification and validation of specialized ELISA assays that will act as a gold standard for brain trauma detection Performed in-process QC and generate performance characteristics data for various sandwich-ELISA assay kits under CGMP environment Assisted with assay development, optimization, and validation of traditional ELISA assays and single molecule immunoassays (Quanterix SimoaTM) Drafted IQ/OQ documentation for routine calibration of BioTek® microplate washer and reader Show less Participated in the product verification and validation of specialized ELISA assays that will act as a gold standard for brain trauma detection Performed in-process QC and generate performance characteristics data for various sandwich-ELISA assay kits under CGMP environment Assisted with assay development, optimization, and validation of traditional ELISA assays and single molecule immunoassays (Quanterix SimoaTM) Drafted IQ/OQ documentation for routine calibration of… Show more Participated in the product verification and validation of specialized ELISA assays that will act as a gold standard for brain trauma detection Performed in-process QC and generate performance characteristics data for various sandwich-ELISA assay kits under CGMP environment Assisted with assay development, optimization, and validation of traditional ELISA assays and single molecule immunoassays (Quanterix SimoaTM) Drafted IQ/OQ documentation for routine calibration of BioTek® microplate washer and reader Show less



La Jolla Institute for Allergy and Immunology

• Research Technician

  • Mar 2016 - Sep 2016

  Facilitate a genome-wide screening by utilizing the genome wide CRISPR-Cas9 Knock out (GeCKO) pooled library system to create single-gene knockout neutrophil-like cells (using HL60 cell line) in order to discover genes regulating integrin activation Utilize BSL-2 lentiviral vectors to produce and harvest lentivirus through transfection of HEK293T cells via lipofection Analyze viral titer determination methods such as flow cytometry (via BD-FACSCANTO II), cell viability, and ELISA… Show more Facilitate a genome-wide screening by utilizing the genome wide CRISPR-Cas9 Knock out (GeCKO) pooled library system to create single-gene knockout neutrophil-like cells (using HL60 cell line) in order to discover genes regulating integrin activation Utilize BSL-2 lentiviral vectors to produce and harvest lentivirus through transfection of HEK293T cells via lipofection Analyze viral titer determination methods such as flow cytometry (via BD-FACSCANTO II), cell viability, and ELISA assay Optimize intracellular staining for flow cytometry samples to detect presence of Cas9 nuclease after viral transduction Maintain multiple cell lines, both adherent and suspension cells, through sterile cell culture techniques Show less Facilitate a genome-wide screening by utilizing the genome wide CRISPR-Cas9 Knock out (GeCKO) pooled library system to create single-gene knockout neutrophil-like cells (using HL60 cell line) in order to discover genes regulating integrin activation Utilize BSL-2 lentiviral vectors to produce and harvest lentivirus through transfection of HEK293T cells via lipofection Analyze viral titer determination methods such as flow cytometry (via BD-FACSCANTO II), cell viability, and ELISA… Show more Facilitate a genome-wide screening by utilizing the genome wide CRISPR-Cas9 Knock out (GeCKO) pooled library system to create single-gene knockout neutrophil-like cells (using HL60 cell line) in order to discover genes regulating integrin activation Utilize BSL-2 lentiviral vectors to produce and harvest lentivirus through transfection of HEK293T cells via lipofection Analyze viral titer determination methods such as flow cytometry (via BD-FACSCANTO II), cell viability, and ELISA assay Optimize intracellular staining for flow cytometry samples to detect presence of Cas9 nuclease after viral transduction Maintain multiple cell lines, both adherent and suspension cells, through sterile cell culture techniques Show less



Targeson Inc

• United States
• Construction
• 1 - 100 Employee

• Research Associate

  • Jul 2015 - Feb 2016

  Performed the manufacturing process, optimization and development of first-in-class microparticle reagent for buoyancy-based cell isolation Assisted group leader with test article characterization, experimental design, data analysis, development of novel lipid microparticle systems for cell-based assays – cell separation, transfection, and imaging Participated in pre-clinical development and qualification (utilizing receptor-ligand affinity ELISA) of TS-07-009, a molecular imaging… Show more Performed the manufacturing process, optimization and development of first-in-class microparticle reagent for buoyancy-based cell isolation Assisted group leader with test article characterization, experimental design, data analysis, development of novel lipid microparticle systems for cell-based assays – cell separation, transfection, and imaging Participated in pre-clinical development and qualification (utilizing receptor-ligand affinity ELISA) of TS-07-009, a molecular imaging agent for detection of acute cardiac disease Drafted SOP and technical reports for various manufacturing processes involving novel lipid microparticle Maintained lab equipments (scheduled calibration) and managed lab supply orders Show less Performed the manufacturing process, optimization and development of first-in-class microparticle reagent for buoyancy-based cell isolation Assisted group leader with test article characterization, experimental design, data analysis, development of novel lipid microparticle systems for cell-based assays – cell separation, transfection, and imaging Participated in pre-clinical development and qualification (utilizing receptor-ligand affinity ELISA) of TS-07-009, a molecular imaging… Show more Performed the manufacturing process, optimization and development of first-in-class microparticle reagent for buoyancy-based cell isolation Assisted group leader with test article characterization, experimental design, data analysis, development of novel lipid microparticle systems for cell-based assays – cell separation, transfection, and imaging Participated in pre-clinical development and qualification (utilizing receptor-ligand affinity ELISA) of TS-07-009, a molecular imaging agent for detection of acute cardiac disease Drafted SOP and technical reports for various manufacturing processes involving novel lipid microparticle Maintained lab equipments (scheduled calibration) and managed lab supply orders Show less



FFA Sciences

• United States
• Biotechnology Research
• 1 - 100 Employee

• Graduate Student Intern

  • Apr 2015 - Jun 2015

  Optimized storage buffer chemistry of fatty acid detection probes used during lyophilization to improve probe recovery rate after resuspension Performed protein purification (FPLC through BioLogic DuoFlow System from Bio-Rad) and reaction chemistry to generate needed probes for testing Optimized storage buffer chemistry of fatty acid detection probes used during lyophilization to improve probe recovery rate after resuspension Performed protein purification (FPLC through BioLogic DuoFlow System from Bio-Rad) and reaction chemistry to generate needed probes for testing



Henkel of America

• Medical Equipment Manufacturing
• 1 - 100 Employee

• Technical Specialist

  • Oct 2013 - Aug 2014

  Resolved processing issues related to out-of-autoclave carbon fiber reinforced polymer (CFRP) fabrication Optimized the process and cure profile for vacuum assisted resin infusion method (CFRP fabrication) Trained incoming technicians on basic out-of-autoclave carbon composite fabrication knowledge

• Lab Technician

  • Sep 2011 - Oct 2013

  Fabricated, prepared, and tested specimen (next generation pre-impregnated ‘Prepreg’ CFRP) Participated in product qualification per aerospace specifications and executed specialized SOP