Experience

Institute of Molecular and Clinical Ophthalmology Basel (IOB)

• Switzerland
• Research Services
• 1 - 100 Employee

• Research assistant

  • Jun 2023 - Present

  Ophthalmic Translational Research Group headed by Bence György Team is working on rare genetic diseases like Stargardt, Usher syndrome. Techniques : - Cell culture : human iPSCs, human retinal organoids (maintenance, amplification, freezing/thawing) - Cryosectionning, staining, imaging (Spinning-disk) - Molecular biology Organization of the lab: - Stock management Ophthalmic Translational Research Group headed by Bence György Team is working on rare genetic diseases like Stargardt, Usher syndrome. Techniques : - Cell culture : human iPSCs, human retinal organoids (maintenance, amplification, freezing/thawing) - Cryosectionning, staining, imaging (Spinning-disk) - Molecular biology Organization of the lab: - Stock management



Inserm

• France
• Research Services
• 700 & Above Employee

• Research Assistant

  • Oct 2021 - May 2023

  Team: Laboratoire de Génétique Médicale (LGM) INSERM U1112 headed by Pr. Hélène Dollfus. The Team is working on rare genetic diseases (Bardet-Biedel, Alström, …) I’m in charge of the technical management of a new project : - Biopsy : Culturing of patients’s biopsies to obtain fibroblasts : testing of different protocoles - Cell culture on fibroblasts : maintenance, amplification, freezing/thawing - Fix problem with mycoplasma - Introduce hiPSCs cells in the lab : maintenance, amplification, freezing/thawing, reprogramming - Differenciation of iPSCs to obtain organoids (retina) - ICC, qPCR - Managing administrative documents : MTA, SORI, CODECOH, report from consorsium’s meeting, regular interaction with the different consortium’s teams Organization of lab : - Stock and order management - Introduce rules and write procedures, in the lab and in the BL2 - Health and safety responsible Show less



Au singulier

• France
• Graphic Design
• 1 - 100 Employee

• Personal break

  • Jun 2017 - Oct 2021

  Personal break to create my own brand in Fashion Personal break to create my own brand in Fashion



CNRS - Centre national de la recherche scientifique

• Research assistant

  • Oct 2016 - Jun 2017

  Institute of Cellular and Integrative Neurosciences (INCI) Team : Study of molecular mechanisms involving exocytosis-endocytosis coupling in neuroendocrine cells and their implication in cancer, headed by Dr Stéphane Gasman and Dr Nicolas Vitale Purification of two proteins to study their interactions at the plasma membrane level. Institute of Cellular and Integrative Neurosciences (INCI) Team : Study of molecular mechanisms involving exocytosis-endocytosis coupling in neuroendocrine cells and their implication in cancer, headed by Dr Stéphane Gasman and Dr Nicolas Vitale Purification of two proteins to study their interactions at the plasma membrane level.



Université de Strasbourg

• Chemical Manufacturing
• 1 - 100 Employee

• Research assistant

  • Mar 2016 - Sep 2016

  Laboratoire de Neurosciences Cognitives et Adaptatives (LNCA) Team : Neurobiology of cognitive decline, headed by Dr. Chantal Mathis Immunohistochemistry on mice brain sections to quantify the neuronal activation after a memory test to study Alzheimer’s disease Technical: - immohistochemistry - confocal miscroscopy - vibratome Laboratoire de Neurosciences Cognitives et Adaptatives (LNCA) Team : Neurobiology of cognitive decline, headed by Dr. Chantal Mathis Immunohistochemistry on mice brain sections to quantify the neuronal activation after a memory test to study Alzheimer’s disease Technical: - immohistochemistry - confocal miscroscopy - vibratome



IGBMC

• Research Services
• 200 - 300 Employee

• Engineer

  • Oct 2014 - Feb 2016

  Department : Development and stem cellsTeam : Muscle and vertebra development, headed by Dr. Olivier PourquieInduction of mutations in hIPS to study the Duchenne muscular dystrophy using CRISPR Cas9Technical :- hIPS cells culture : maintenance (cutting), transfection, muscle fiber differentiation- Immunocytochemistry- DNA/RNA extraction- RT-PCR, qPCR- Western-blot- knowledge in FACSI co-authored in the resulting publication: Chal et al., 2015. Differencation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy. Nat Biotechnol. Show less

• Engineer

  • Jun 2014 - Oct 2014

  Department : Development and stem cellsTeam : Differentiation and physiopathology of endocrine cells in the pancreas and intestine, headed by Dr. Gérard GradwohlTechnical :- Management and genotyping of mice models- DNA/RNA extraction- Cell culture (Hela, Hek-293T cell lines)- Islets of Langerhans purification from mice / intraperitoneal injections.- RT-PCR, qPCR- Western-blot- Cloning



Université de Lorraine

• France
• Higher Education
• 700 & Above Employee

• Technician

  • Oct 2013 - Mar 2014

  Team : Cibles thérapeutiques, formulation et expertise préclinique du médicament Study of PDI (Protein disulfide isomerase) activity and expression Technical: - Western-blot on cell and tissue extracts (liver and aorta) - Synthesis of a substrate to measure the PDI activity. - Cell culture (A10 cell line) - Knowledge in HPLC Team : Cibles thérapeutiques, formulation et expertise préclinique du médicament Study of PDI (Protein disulfide isomerase) activity and expression Technical: - Western-blot on cell and tissue extracts (liver and aorta) - Synthesis of a substrate to measure the PDI activity. - Cell culture (A10 cell line) - Knowledge in HPLC



Prenyl BIO SAS

• Technician internship

  • Feb 2013 - Aug 2013

  Department of cosmetic products formulation, headed by Dr. Bénédicte Cantecor Cosmetics R&D: Design of a new preservative for a range of cosmetic products Department of cosmetic products formulation, headed by Dr. Bénédicte Cantecor Cosmetics R&D: Design of a new preservative for a range of cosmetic products

• Technician internship

  • Jan 2011 - Feb 2011

  Team: Molecular biology platform, headed by Dr Didier Busso. Construction of an expression vector: Cloning reaction / SLIC technical Team: Molecular biology platform, headed by Dr Didier Busso. Construction of an expression vector: Cloning reaction / SLIC technical

• Technician internship

  • May 2010 - Jul 2010

  Team U692: laboratory of molecular signaling and neurodegeneration, headed by Dr Jean-Philippe Loeffler Study of dynein functions in mice embryonic striatal neurons. Technical: - Immunocytochemistry on mice striatal neurons - Western-Blot. I am acknowledged for my work in the following publication: Braunstein et al., 2010. A point mutation in the dynein heavy chain gene leads to striatal atrophy and compromises neurite outgrowth of striatal neurons. Hum Mol Genet. 19:4385-4398. Show less