Experience

Ksilink
• France
• Biotechnology Research
• 1 - 100 Employee
• Senior Associate Scientist

  • Jan 2023 - Present


Dynacure

• France
• Biotechnology Research
• 1 - 100 Employee

• Senior Associate Scientist

  • Dec 2021 - Nov 2022

  Dynacure is a clinical-stage company focused on developing and commercializing novel therapies to transform the lives of patients with rare diseases who have limited or no treatment options. Dynacure is developing DYN101, an investigational antisense product candidate designed to reduce the expression of dynamin 2 protein for the treatment of Myotubular and Centronuclear Myopathies, in strategic collaboration with Ionis Pharmaceuticals. In this company, I was in charge of the evaluation of DNM2 and MTM1 gene modulation impact on muscle cell phenotype. To this end, I set up the culture of immortalized human skeletal muscle myoblasts and checked their proliferation and differentiation status to select best clones (IF and qRT-PCR). Then, I optimized ASO electroporation and siRNAs transfection experiments to down regulate specific genes (IF, qRT-PCR and WB). I also edited mutations on a DNM2 plasmid to further evaluate the impact of these mutations on the cell phenotype. For this purpose, I also optimized plasmid lipofection experiments. At the same time, I developed several immunostainings (on coverslips) to access the cell phenotype. I was able to share and discuss all these protocols with a CRO to develop a multiple screening assay. At the lab level, I actively help to generate new databases (primers, antibodies, cells), I was responsible for the L2 culture room and I mentored a technician for a year. Show less



Anagenesis Biotechnologies

• France
• Biotechnology

• Ingénieur

  • Nov 2014 - Dec 2021

  Anagenesis is a preclinical‐stage stem cell-based company focused on developing novel treatments for genetic and age-related muscle degenerative diseases with unmet medical needs. In this company, I performed hIPS culture and differentiation toward muscle lineage to obtain muscle progenitor cells. I took part in the miniaturization of this process in order to perform a high throughput drug screening. To this end, I developed and validated semi-automated programs on a pipetting head to plate/dilute cells and compounds onto 384 well-plates and to perform IF. I highly contributed to cell imaging analysis program optimization on MetaXpress. I was in charge of performing all experiments and analyzing the data generated in the main SAR project of the lab. As the accuracy and reproducibility were crucial, I focused on standardizing and recording all processes of this project (SOP writing for cell culture, compounds resuspension, analyses, batch tracking…) and I mentored an engineer assistant for a year. As the aim was to validate these compounds in mice, I was involved in CROs discussions concerning experiments design and follow-up and I supervised one technician on muscle force measurement following compound injection. Beside this main project, I also developed other cell-based assays and carried out qRT PCR, cell sorting (FACS and MACS), cell viability/toxicity, cryosections and ELISA experiments. Finally, I was responsible for creating and updating primers, compounds, cells and antibodies databases. Show less



IGBMC

• Research Services
• 200 - 300 Employee

• Assistant ingénieur

  • Nov 2010 - Nov 2014

  I was involved in a starting project which consisted to establish a protocol and to highlight molecules able to differentiate embryonic stem cells into muscular progenitors and fibers. I performed and optimized many cell culture protocols on mouse/human embryonic stem cells and on pluripotent induced stem cells. I was responsible for the cell culture room (health and safety, stock avaibility) and for the newcomers training. I was formed to molecular biology and performed cloning, bacteria amplification, PCR, RT-qPCR. I had the opportunity to create RNA probes and to achieve in situ hybridization on mouse embryos. I carried out animal experimentations as comportemental tests, grafts, or mouse/chicken embryos dissection. I achieved organs inclusion (paraffin, OCT) and section (cryostat, microtome). I was then involved in the generation of reporter cell lines by CRISPR/Cas9 method on human pluripotent stem cells. I electroporated cells, selected clones and confirmed the reporter insertion by PCR. I used and was autonomous on several instruments like cell analyzer (FACS Calibur, LSRII), cell sorter (S3 cell sorter, FACS ARIA), microscope (Evos, Zeiss, Leica), PCR or RT-qPCR machine (LC480). I edited and maintained data banks/bases to register qPCR primers and RNA probes. I elaborated excel, power point or word files to summarise experiments results. Show less



EFS

• Transportation, Logistics, Supply Chain and Storage
• 1 - 100 Employee

• Stage de licence professionnelle

  • Feb 2010 - Sep 2010

  I was in charge of finding and achieving a protocol allowing differentiation of human hematopoietic stem cells into megacaryocytes and into platelets. I perfomed cell culture and immunofluorescence. I was formed to cytospin and cell analyser use. I also isolated hematopoietic stem cells from mice bone marrow using MACS technique and differentiated it in in vitro. I helped others technicians and ingeneers on their project performing cloning or plasmid isolation. I was in charge of finding and achieving a protocol allowing differentiation of human hematopoietic stem cells into megacaryocytes and into platelets. I perfomed cell culture and immunofluorescence. I was formed to cytospin and cell analyser use. I also isolated hematopoietic stem cells from mice bone marrow using MACS technique and differentiated it in in vitro. I helped others technicians and ingeneers on their project performing cloning or plasmid isolation.