Experience

l'institut du thorax

• France
• Think Tanks
• 1 - 100 Employee

• Researcher

  • Feb 2019 - Present

  • High-throughput characterization of genetic variants on cardiac ion channels activity. • In-depth knowledge in molecular biology with experience in designing and performing molecular cloning of plasmids. Over 70 mutant plasmids constructed between 2019 to 2020. • Knowledge and use of various molecular biology techniques, including nucleic acid and protein extraction, PCR, DNA sequencing, electrophoresis. • Experience with cell culture and cellular assays, different transfection methods of plasmids of interest, cytotoxicity and apoptosis, immunofluorescence, flow cytometry, confocal and fluorescence microscopy. • Experience in conventional and automatic patch clamp techniques. Show less



Universidade Federal de Minas Gerais

• Brazil
• Higher Education
• 700 & Above Employee

• Researcher

  • Apr 2015 - Jan 2019

  • Discovery and characterization of new natural molecules with pharmacological and biotechnological interest. • Investigation of mechanisms of action of natural products with arrhythmic effect and calcium signaling in cardiomyocytes by activation of intracellular ion channels. • Experience with confocal microscopy on living and fixed cells, siRNA knockdown and nanoparticle-mediated adenoviral transduction in primary cardiac muscle cells. • Use of cell-penetrating peptides to deliver molecules and nanoparticles to the intranuclear region of tumor cells. A patent has been assigned for this new molecular delivery tool. • Experience in animal models. Use of toxicity assays, pharmacological tests, distribution and delivery studies of molecules in vivo. • Development of diagnostic tests using nanotechnology, with patent filing. • Production, expression and purification of recombinant proteins in bacterial system. Experience in prokaryotic cell culture and microbiology assays. • Management of animal models for immunization and antibody production. • Knowledge in protein biochemistry and immunology. Experience in high performance liquid chromatography, mass spectrometry, western blot, ELISA. • Elaboration of technical and scientific reports. Participation and organization of scientific events. • Coordination of activities and technical team for the operationalization of the development of new products requested. • Capacity to apply expertise to execute experiments in a time-sensitive manner. Show less