Brian Sukovich
Advanced Research Associate at R&D Systems- Claim this Profile
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Bio
Experience
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R&D Systems
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United States
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Biotechnology Research
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300 - 400 Employee
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Advanced Research Associate
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Sep 2019 - Present
- Coordinates weekly workflow for a team of three research associates. - Purify a wide variety of recombinant proteins associated with the Ubiquitin Proteasome System expressed in bacterial and insect cell lines. - Developed expression, purification and conjugation protocols for custom projects and proteins not associated with the UPS. - Further developed and optimized protocols for top-selling conjugates, reducing production time and raising efficiency. - Characterize enzymatic activity using qualitative SDS-PAGE assays in conjunction with plate-based fluorescence and luminescence assays for quantitative analysis. - Trained new employees on the use of FPLC and HPLC systems, purification methods, QC assays, and protocols for generating reports. Show less
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UMass Boston
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United States
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Higher Education
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700 & Above Employee
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Research Assistant
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Jun 2017 - Present
Assisted PhD candidate in study of trace element analysis of blue mussel populations off of the New England Coast. Currently involved in a study of isotope integration from prey organisms in native and invasive crab species off of the New England Coast. Lab Techniques: Histology, Microscopy, Tissue Fixation, Bivalve Husbandry
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Associate Lecturer
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Sep 2018 - Sep 2019
Laboratory teacher for General Biology I & II, focusing on the fundamental concepts of Biology and their associated laboratory techniques.Lab Techniques Utilized: DNA Extraction, Chromatography, PCR, Protein Purification
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Massachusetts General Hospital
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United States
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Hospitals and Health Care
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700 & Above Employee
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ED Registrar
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Apr 2014 - Sep 2019
Collect and verify patient demographic information; Ensure insurance information is coded accurately; Refer patients to departments that would assist in signing up for health insurance or provide other payment options Recipient of the Partners in Excellence Award in 2014 Collect and verify patient demographic information; Ensure insurance information is coded accurately; Refer patients to departments that would assist in signing up for health insurance or provide other payment options Recipient of the Partners in Excellence Award in 2014
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Best Buy
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Retail
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700 & Above Employee
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Sales Associate
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2010 - Apr 2014
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Education
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University of Massachusetts Boston
Bachelor of Science - BS, Biology