Experience

BGI Group

• China
• Biotechnology Research
• 1 - 100 Employee

• Laboratory Scientist

  • Aug 2019 - Present



Ontera

• United States
• Biotechnology Research
• 1 - 100 Employee

• Analytical Chemistry & Applications Scientist

  • Jan 2018 - Present

  My roll at Ontera is to create, purify, & verify various DNA-peptide, DNA-Protein, and peptide nucleic acid bioconjugates for use in our single molecule bio-detection and analysis platform. My roll at Ontera is to create, purify, & verify various DNA-peptide, DNA-Protein, and peptide nucleic acid bioconjugates for use in our single molecule bio-detection and analysis platform.



University of California, San Francisco

• United States
• Higher Education
• 700 & Above Employee

• Associate Specialist

  • Jan 2012 - Aug 2017

  I am a mass spectrometry and proteomics specialist in the Thermo Fisher Scientific Proteomics Facility for Disease Target Discovery at the Gladstone Institutes & University of California, San Francisco. My responsibilities include operation and maintenance of Orbitrap Elite and Fusion instruments, as well as developing and using state of the art techniques for the preparation of mass spectrometry samples. Nevan Krogan’s Lab is primarily involved in using unbiased systems based approaches to study the mechanisms of human diseases such cancer, HIV/AIDS, and influenza. To this end, the Lab uses mass spectrometry to study protein-protein interactions and conduct large scale profiling of post translational modifications of the cellular proteome in the presence of disease states or factors. My contributions include developing and optimizing the sample preparation protocols for the enrichment of phosphorylated, ubiquitylated, or acetylated peptides. I have experience preparing a variety of biological samples including those from cell culture, tissue, and serum. I have applied my protocols to SILAC, TMT, iTRAQ, or Label-free quantitative mass spectrometry approaches. I conduct searching of the raw MS data with MaxQuant software and interface with bioinformatics specialists in the to perform further statistical analysis. Taking samples from the cell culture room all the way through the mass spec and to interpretation of raw data have given me key insights into facilitating the discovery process such as:• Ensuring collaborators understand key elements of mass spectrometry experimental design.• Understanding what techniques and methodologies have the best chance of being successful.• Ensuring scientists and bioinformaticists have needed components to complete downstream analysis• Understanding where to focus trouble shooting and optimization when problems arise.• Understanding the hardware and software infrastructure requirements for MS installations. Show less

• Associate Specialist

  • Jan 2012 - Aug 2017



Texas A&M University

• United States
• Higher Education
• 700 & Above Employee

• Graduate Research Assistant, PhD

  • May 2008 - Nov 2011

  • Thesis Title: Proteomics of Oxidative Stress using Inducible CYP2E1 Expressing HepG2 Cells and 3T3-L1 Adipocytes as Model Systems• Research Overview 1: Conducted quantitative proteome profiling of differentiated and hypertrophic 3T3-L1 Adipocytes using iTRAQ based peptide tagging, novel liquid phase sample fractionation, and MALDI tandem mass spectrometry. • Project results: Identified over 600 and quantifies 122 proteins in a 6 sample time-course. • Determined up-regulation of TCA cycle and fatty acid oxidation proteins in enlarging adipocytes. • Identified key mitochondrial targets that lead to enhanced oxidative stress in hypertrophic adipocytes.• Research Overview 2: Development of inducible cytochrome p450 2E1 expressing hepatocyte cell line to better characterize the role of enhanced oxidative stress in hepatocyte dysfunction. • Project results: Development of CYP2E1 expression plasmid using the pRevTRE system and establishment of a stable, inducible cell line allows for controlled CYP2E1 expression • Greater sensitivity to oxidative stress, shown by greater toxicity and lower glutathione after exposure to acetaminophen, as compared to constitutive CYP2E1 expressing line. • Potential for pre-treatment and pre-labeling of cells prior to CYP2E1 induction. Show less

• Graduate Research Assistant, MS

  • Jan 2005 - May 2008

  • Thesis Title: Proteomic Study of Oxidative Stress in Alcoholic Liver Disease • Research Overview: Conducted two dimensional gel electrophoresis follow by MS/MS based protein identification for the proteomic study of an animal model for early stage alcoholic liver disease. • Project results: Identified betaine-homocysteine S-methyltransferase and decreased expression of anti-oxidant enzymes as potential biomarkers for early stage ALD. Show less



Shriners Children's

• Hospitals and Health Care
• 700 & Above Employee

• Summer Scholars Intern

  • Jun 2006 - Aug 2006

  • Used COMSOL FEM and digital image tracking software to study flow of blood cells in very small channels. • Used COMSOL FEM and digital image tracking software to study flow of blood cells in very small channels.



Lynntech, Inc.

• United States
• Research Services
• 1 - 100 Employee

• Research Engineer

  • Mar 2002 - Oct 2004

  • Developed and optimized fully composite direct methanol fuel cell. Developed one piece injection molded polymer and graphite frame & flow-fields and matching one piece PEM electrode assemblies • Conducted primary research on a ammonia-borane based alkaline fuel cell with non-precious metal based anode catalyst. Wrote DOD SBIR award winning proposal based on this work. • Developed and optimized fully composite direct methanol fuel cell. Developed one piece injection molded polymer and graphite frame & flow-fields and matching one piece PEM electrode assemblies • Conducted primary research on a ammonia-borane based alkaline fuel cell with non-precious metal based anode catalyst. Wrote DOD SBIR award winning proposal based on this work.



Alcoa

• United States
• Mining
• 700 & Above Employee

• Process Engineer

  • Jan 2001 - Dec 2001

  • Process engineering and technical support to alumina refining precipitation circuit. • Process engineering and technical support to alumina refining precipitation circuit.