Experience

Dewpoint Therapeutics

• United States
• Biotechnology Research
• 100 - 200 Employee

• Senior Research Associate

  • Feb 2020 - Present

  PROJECT GOAL: CHARACTERIZE THE MECHANISMS BY WHICH STRESS GRANULES SEQUESTER AND MODULATE INTRINSICALLY DISORDERED PROTEIN (TDP43) CONDENSATE DYNAMICS · Identified TDP43-knockdown effects on pathological biomarkers through siRNA in neuroblastoma and primary neuronal cultures · Investigated the effect of neuroblastoma differentiation on stress tolerance and TDP43 granule formation · Developed novel cell lines to investigate TDP43 aggregation and stress granule dynamics (formation, fusion, and dissolution) in the presence of stressors of various biochemical mechanisms · Studied the effects of nuclear-cytoplasmic transport mechanisms in TDP43 stress granule sequestration and phosphorylation · Studied the biomolecular dynamics of TDP43 formation and phosphorylation in the presence of stress granules in both over-expression and endogenous in vitro systems



Aquinnah Pharmaceuticals

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Associate II

  • May 2018 - Feb 2020

  PROJECT GOAL: CHARACTERIZE THE EFFECTS OF GENETIC AND PATHOLOGICAL BIOMARKERS ON INTRINSICALLY DISORDERED PROTEIN (TAU) PROPAGATION AND SEQUESTRATION INTO CONDENSATES · Developed novel cell lines to investigate tau propagation and stress granule dynamics (formation, fusion, and dissolution) · Studied the biomolecular dynamics of tau formation and phosphorylation in the presence of stress granules in both over-expression and endogenous in vitro systems · Identified tau-knockdown effects on stress granules and pathological biomarkers through siRNA in primary neuronal cultures · Studied resulting genomic alterations in the presence of mechanistically differing stressors; identified links to pathology · Investigated the relationship between pathological biomarkers and genomic alterations in vivo



Bradley Hyman Lab

• MGH-Harvard Medical, Charlestown, MA

• Undergraduate Researcher

  • Jun 2016 - May 2018

  PROJECT GOAL: CHARACTERIZE THE EFFECTS OF GENETIC AND PATHOLOGICAL BIOMARKERS ON INTRINSICALLY DISORDERED PROTEIN (TAU) DEPOSITION AND PROPAGATION IN TAUOPATHIES (HUMAN AND MURINE TISSUE) · Helped characterize a novel tau reduction strategy in vivo utilizing engineered zinc finger protein transcription factors · Studied biochemical mechanisms of tau deposition and propagation in Alzheimer’s human and rodent brain lysate · Investigated tau aggregation and tau-knockdown methods in vitro and in vivo in primary tauopathies · Studied tau reduction in the presence of Amyloid ß deposition to identify complementary/compensatory mechanisms · Created and analyzed genetic profiles for isolated single-nuclei RNA with 10x Genomics



Yajaira Suarez Lab

• Yale University, New Haven, CT

• Summer Intern

  • May 2015 - Aug 2015

  · Studied exosome transfer in cancer cells and injected mice (subcutaneous, tail vein, retro-orbital) · Created and screened guide RNA sequences in vitro for a CRISPR Cas9 directed therapy against tumor formation · Studied exosome transfer in cancer cells and injected mice (subcutaneous, tail vein, retro-orbital) · Created and screened guide RNA sequences in vitro for a CRISPR Cas9 directed therapy against tumor formation



Themis Kyriakides Lab

• Yale University, New Haven, CT

• Summer Intern

  • May 2014 - Aug 2014

  · Studied the effect of IL-4 on Macrophage Fusion in the foreign body response · Studied the effect of IL-4 on Macrophage Fusion in the foreign body response