Bianca de Freitas Machado

Cell Culture Specialist - Research Assistant at denovoMATRIX
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Contact Information
us****@****om
(386) 825-5501
Location
Dresden, Saxony, Germany, DE
Languages
  • English Native or bilingual proficiency
  • Spanish Full professional proficiency
  • Portuguese Native or bilingual proficiency
  • German Professional working proficiency

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Credentials

  • IELTS Academic
    IELTS Official
    Mar, 2020
    - Nov, 2024
  • TOEFL ITP
    The TOEFL® test
    Oct, 2016
    - Nov, 2024
  • CAE - Cambridge English: Advanced
    Cambridge College
    Jul, 2013
    - Nov, 2024
  • DELE - Diploma de Español como Lengua Estranjera
    Instituto Cervantes
    Dec, 2011
    - Nov, 2024
  • FCE - First Certificate in English
    Cambridge College
    Dec, 2011
    - Nov, 2024

Experience

  • denovoMATRIX
    • Germany
    • Biotechnology Research
    • 1 - 100 Employee
    • Cell Culture Specialist - Research Assistant
      • Aug 2023 - Present

      § Responsible for stem cell culture procedures, ensuring optimal growth conditions and maintaining iPSC cell lines. § Collaborating in the development and optimization of stem cell-based protocols on chemically defined coatings. § Responsible for stem cell culture procedures, ensuring optimal growth conditions and maintaining iPSC cell lines. § Collaborating in the development and optimization of stem cell-based protocols on chemically defined coatings.

  • Technische Universität Dresden
    • Germany
    • Research
    • 700 & Above Employee
    • Research Assistant
      • Nov 2022 - May 2023

      Evaluating the chondrogenic differentiation potential of hMSC in an artificial extracellular matrix (aECM) scaffold cultivated in dynamic biophysical niches. Evaluating the chondrogenic differentiation potential of hMSC in an artificial extracellular matrix (aECM) scaffold cultivated in dynamic biophysical niches.

  • Technische Universität Dresden
    • Germany
    • Research
    • 700 & Above Employee
    • Master Thesis Project
      • Apr 2022 - Sep 2022

      § Conducted in-depth research to investigate the influence of various biological cues on the differentiation potential of human mesenchymal stem cells (hMSCs) in collagen-based scaffolds. § Utilized RT-qPCR to analyze gene expression patterns, providing valuable insights into cellular responses to different cues. § Employed Scanning Electron Microscopy to assess scaffold morphology, contributing to a comprehensive understanding of the cellular microenvironment § Conducted in-depth research to investigate the influence of various biological cues on the differentiation potential of human mesenchymal stem cells (hMSCs) in collagen-based scaffolds. § Utilized RT-qPCR to analyze gene expression patterns, providing valuable insights into cellular responses to different cues. § Employed Scanning Electron Microscopy to assess scaffold morphology, contributing to a comprehensive understanding of the cellular microenvironment

  • Universitätsklinikum Carl Gustav Carus
    • Germany
    • Medical Practices
    • 300 - 400 Employee
    • Research Assistant
      • Apr 2022 - Sep 2022

      § Performed mice genotyping, protein assays and RT-qPCRs to analyze gene expression, providing critical molecular insights into ongoing research projects. § Performed mice genotyping, protein assays and RT-qPCRs to analyze gene expression, providing critical molecular insights into ongoing research projects.

  • Universitätsklinikum Carl Gustav Carus
    • Germany
    • Medical Practices
    • 300 - 400 Employee
    • Student Researcher
      • Sep 2021 - Feb 2022

      § Conducted research to evaluate the impact of mutated nucleic acid-sensing pathways on the expression levels of IFN-stimulated genes in HeLa cells, utilizing RT-qPCR as a critical molecular analysis tool. § Performed siRNA transfection techniques to effectively silence specific genes suspected of interfering with inflammatory response patterns. § Conducted research to evaluate the impact of mutated nucleic acid-sensing pathways on the expression levels of IFN-stimulated genes in HeLa cells, utilizing RT-qPCR as a critical molecular analysis tool. § Performed siRNA transfection techniques to effectively silence specific genes suspected of interfering with inflammatory response patterns.

  • Technische Universität Dresden
    • Germany
    • Research
    • 700 & Above Employee
    • Student Researcher - Center for Regenerative Therapies Dresden (CRTD)
      • Apr 2021 - Aug 2021

      § Conducted in-situ hybridization of cryosectioned zebrafish fin tissues to assess the role of osteal macrophages in bone remodeling, contributing to a deeper understanding of tissue-specific interactions and cell behavior after injury

    • Student Researcher - Center for Translational Bone, Joint and Soft Tissue Research - MTZ
      • Oct 2020 - Mar 2021

      § Performed 3D Melt-Electrospinning Writing techniques to create composite P(LA-CL) scaffolds and subsequently cultivated human mesenchymal stem cells (hMSCs) on these scaffolds. § Conducted continuous imaging via Confocal Fluorescence Microscopy over a 30-day period to monitor cell behavior and orientation tissue development, contributing to the investigation of long-term cell-scaffold interaction.

  • USP - Universidade de São Paulo
    • Brazil
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Student Researcher - Plastics and Skin Reconstruction Lab
      • Oct 2018 - Jun 2020

      § Focused on skin tissue engineering, with an emphasis on the extraction and co-culture of human adipose-derived stem cells and fibroblasts, contributing to a deeper understanding of cellular interactions within the skin microenvironment. § Led the development of hydrogel-based decellularized skin scaffolds for cell culture, providing valuable insights into tissue engineering techniques and biomaterials. § Actively participated in the development of an animal model for skin injury recovery, playing a key role in the design and execution of experiments aimed at assessing the regenerative potential of novel treatments and therapies. Show less

  • Institute of Biomedical Sciences - University of Sao Paulo
    • Brazil
    • Higher Education
    • 100 - 200 Employee
    • Museum Educator - Human Anatomy Museum
      • Sep 2018 - Feb 2019

      Academic Program Intern at the Human Anatomy Museum "Professor Alfonso Bovero", acting as a museum educator while informing visitors about the collections through tours. Responsible for developing and running educational activities for museum visitors. Academic Program Intern at the Human Anatomy Museum "Professor Alfonso Bovero", acting as a museum educator while informing visitors about the collections through tours. Responsible for developing and running educational activities for museum visitors.

  • USP - Universidade de São Paulo
    • Brazil
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Student Researcher - Sepsis Neuroimmunology Laboratory
      • Aug 2015 - May 2018

      § Conducted research on macrophage signaling during the early stages of inflammation, contributing to a comprehensive understanding of the immune response. § Cultivated murine tissue-derived macrophages and performed detailed analysis and subsetting of macrophages using flow cytometry, providing critical insights into their diverse functions. § Developed in vitro conditioned-medium experiments to evaluate the effects of inflammatory responses mediated by tissue-resident macrophages, furthering our knowledge of cellular interactions. § Utilized enzyme immunoassays (ELISA) to quantify inflammatory mediators, adding quantitative data to the investigation of inflammation and macrophage signaling. Show less

Education

  • Technische Universität Dresden
    Master of Science - MS, Regenerative Biology and Medicine
    2020 - 2022
  • Universidade de São Paulo
    Bachelor's degree, Biomedical Sciences, General
    2015 - 2019

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