Analista de Desenvolvimento Tecnológico II

• Nov 2022 - Present

Analista de Pesquisa Júnior

• Mar 2022 - Oct 2022

• Organização de laboratório, preparo de reagentes e soluções, cultivo de células imortalizadas de mamíferos, cultura primária de queratinócitos humanos, produção de modelo 3D de epiderme humana reconstruída in vitro, ensaios de viabilidade celular, testes de irritação cutânea, avaliação de princípios ativos por titulação colorimétrica, elaboração de POP’s e IT’s. • Organização de laboratório, preparo de reagentes e soluções, cultivo de células imortalizadas de mamíferos, cultura primária de queratinócitos humanos, produção de modelo 3D de epiderme humana reconstruída in vitro, ensaios de viabilidade celular, testes de irritação cutânea, avaliação de princípios ativos por titulação colorimétrica, elaboração de POP’s e IT’s.

Collaborative researcher

• Jan 2020 - Dec 2021

• Execution of the project “Cytotoxic effects of 6-OHDA in dopaminergic neurons are independent of LRRK2 activation”.• Laboratory organization, preparation of reagents and solutions, immortalized mammalian cells culture, primary neurons culture, manipulation and dissection of animals, immunofluorescence assays, electrophoresis, protein dosage, western blot, biomolecules signaling, introduction to confocal microscopy.

Intern

• Feb 2019 - Dec 2019

• Execution of the project “L556S mutation in DDX3X protein is related to aggregate formation and cytotoxicity in neuroblastoma cell line”.• Preparation of reagents and solutions, immortalized mammalian cells culture, anaerobic microorganisms’ culture, immunofluorescence assays, electrophoresis, protein dosage, western blot, cell transfection.

Undergraduate research project

• Mar 2017 - Dec 2018

• Execution of the project “Study of postnatal development of rat prostate under the effect of hyperglycemia: evaluation of AKT, ERK and MMP2”. • Cell culture, cell migration assays, histological analysis with stereological methods, cell and tissue homogenization, protein extraction and quantification, immunohistochemistry, electrophoresis, western blotting, zymography. • Execution of the project “Study of postnatal development of rat prostate under the effect of hyperglycemia: evaluation of AKT, ERK and MMP2”. • Cell culture, cell migration assays, histological analysis with stereological methods, cell and tissue homogenization, protein extraction and quantification, immunohistochemistry, electrophoresis, western blotting, zymography.