Barbara Ross
PCR Technologist at CBLPath- Claim this Profile
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French -
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Bio
Credentials
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Clinical Laboratory Technologist restricted to Molecular diagnosis/genetic testing
NYSOPAug, 2013- Sep, 2024
Experience
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CBLPath
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Medical Practices
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1 - 100 Employee
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PCR Technologist
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Jul 2012 - Present
Performing PCR and Analysis to inform and diagnose diseases, primarily Leukemia and Lymphoma. Writing SOP's for equipment and techniques, and performing and partial writing of new validations. All work necessary to maintaining a clinical laboratory especially quality assurance. Tests performed include Quantitative real time PCR analysis of BCR-ABL fusions for CML and ALL, fragment analysis and sequencing of Calreticulin and JAK2 exon 12 for other white cell proliferative disorders, Fragment analysis for B-cell and T-cell Lymphomas (IGH, IGK, TCRG, and TCRB) using the Applied Biosystems 3500 capillary electrophoresis instrument, and Microsatellite Instability by PCR using the Promega kit for fragment analysis. Show less
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Montefiore Medical Center
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United States
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Hospitals and Health Care
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700 & Above Employee
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Research Associate
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Mar 2010 - Feb 2012
ECOG Leukemia Reference Lab. A tissue bank for blood and bone marrow of patients with Leukemia. I isolated mono-nuclear cells for storage as cells, and in various media for isolation of DNA, RNA, MiRNA, and Protein for down-line researchers. I also did extractions of DNA, RNA, and Protein for shipment to researchers. Administrative duties included setting up and maintaining patient records, interaction with and instruction of research nurses as to the requirements of the protocols and IRBs necessary for including patient materials, maintaining records and satisfying regulations for the lab as a clinical lab under the monitoring of NY State and CAP requirements. I have NYC fire dept. certification for Laboratory safety, and a Restricted License for Flow Cytometry / Cellular Immunology from the NYS Office of the Professions. Show less
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Columbia University
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United States
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Higher Education
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700 & Above Employee
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Senior Resarch Assistant
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Nov 2005 - Apr 2009
Genotyping, Sequencing, and related procedures in the search for causes of Parkinson Disease and Alzheimer’s. Responsible for processing samples and extracting DNA from Saliva, Brain tissue, and Blood. Ordering, safety management, etc. Included teaching summer students from high school to medical students, new technicians and post-docs. Genotyping, Sequencing, and related procedures in the search for causes of Parkinson Disease and Alzheimer’s. Responsible for processing samples and extracting DNA from Saliva, Brain tissue, and Blood. Ordering, safety management, etc. Included teaching summer students from high school to medical students, new technicians and post-docs.
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Research Worker
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Mar 1992 - Feb 2005
PCR, Sequencing (A.B.I. automated sequence analyzer), computer analysis, and related projects in the location and characterization of genes and their mutations causing diseases such as Spinal Muscular Atrophy, Wilson disease, Retinitis Pigmentosum, Epilepsy, and Autism. Additionally served as a consultant for technical problems in other projects, and developed protocols for new equipment, ie pyrosequencing. Taught all graduate students and other technicians to do DNA sequencing. PCR, Sequencing (A.B.I. automated sequence analyzer), computer analysis, and related projects in the location and characterization of genes and their mutations causing diseases such as Spinal Muscular Atrophy, Wilson disease, Retinitis Pigmentosum, Epilepsy, and Autism. Additionally served as a consultant for technical problems in other projects, and developed protocols for new equipment, ie pyrosequencing. Taught all graduate students and other technicians to do DNA sequencing.
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Education
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1978 - 1988
Columbia University in the City of New York
non degree course work, Molecular Biology and Art -
1971 - 1975
Cornell University
BS, Biological Sciences -
1968 - 1971
Bronx High School of Science
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