Experience

Kestrel Therapeutics
• United States
• Biotechnology
• 1 - 100 Employee
• Senior Scientist

  • Apr 2023 - Present


Skyhawk Therapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist

  • Dec 2021 - Apr 2023

  • Worked in collaboration with Associate Director/Lead scientist and supported the team with various scientific research projects and experiments • Participated in target ID/approach validation and MoA efforts involving the planning, designing, and implementing the experiments for the strategies that modulate target gene splice variants with the genetic mutation tools that led to well informed go/no go decisions for multiple targets • Designed and performed the experiments for gene… Show more • Worked in collaboration with Associate Director/Lead scientist and supported the team with various scientific research projects and experiments • Participated in target ID/approach validation and MoA efforts involving the planning, designing, and implementing the experiments for the strategies that modulate target gene splice variants with the genetic mutation tools that led to well informed go/no go decisions for multiple targets • Designed and performed the experiments for gene expression to identify clinically applicable mRNA splice variants with genetic mutations using relevant endpoints, RT-qPCR, PCR • Established the effect of alternatively spliced isoforms on protein expression by Western /HiBiT/Nanoluc blotting/ELISA/In-Cell Western • Conducted multiple in vitro/or cell-based drug screening assays and small molecule toxicity screening on mammalian (human and mouse) cell lines • Supported the in vivo group with PD studies by measuring the protein depletion in xenograft model • Worked and managed external research activities with providing support and direction for the CROs including sending detailed/optimized numerous assay’s protocols (qPCR, CTG, In-Cell Western), data QCing, analyzing data (established analyses templates) and guided by solving technical and scientific challenges • Managed and provided support to Research Associate and helped with the experimental planning, technical training, execution and supporting their professional growth • Presented ongoing research project updates to Biology and Program teams during weekly/by-weekly meetings • Maintained an accurate and up-to-date electronic notebook, including detailed experimental protocol, materials, and methods • Received multiple awards on making outstanding contributions to Company Culture

• Principal Associate Scientist

  • Jan 2019 - Jan 2022



Curis

• United States
• Biotechnology Research
• 1 - 100 Employee

• Associate Scientist

  • Nov 2007 - Nov 2018

  • Provided target validation support and biomarker identification by planning, designing, and performing many studies for testing hypotheses on mechanism of action of lead oncology compounds from discovery to proof-of -concept stages, evaluated the compound’s effect on a variety of cell signaling pathway markers in both solid and hematological malignancies • Evaluated the potential anti-angiogenesis effect of the compounds using endothelial tube formation assay • Optimizing and… Show more • Provided target validation support and biomarker identification by planning, designing, and performing many studies for testing hypotheses on mechanism of action of lead oncology compounds from discovery to proof-of -concept stages, evaluated the compound’s effect on a variety of cell signaling pathway markers in both solid and hematological malignancies • Evaluated the potential anti-angiogenesis effect of the compounds using endothelial tube formation assay • Optimizing and implementing a Plasma Inhibitory Activity assay for PD biomarker changes in Acute Myeloid Leukemia (AML) and DLBCL cell lines to determine the inhibitor activity n plasma isolated from mice dosed with lead compound • Generated and examined drug-resistant cell line, established stable cell line by transfected lentivirus • Worked with CROs/vendors to generate custom cDNA. Developed the qRT-PCR assay for measuring splice-variant specific transcripts which included determination the long/-short mRNA ratio, optimizing several steps of the RNA extraction, and cDNA synthesis, developing primer-probe sets • Developed analysis PBMC samples from clinical trial to support biomarker development and provided reproducible results to facilitate clinical biomarker studies • Developed and provided the analysis plasma samples from clinical trial for Cytokine/Chemokine Multiplex Assay to guide the clinical biomarker studies • Provided key data was presented at 2015 AACR and 2017 ASH annual meetings • Participated in the data analysis and interpretation, writing reports, and presenting the data to upper management • Maintained of electronic laboratory notebook, including accurate and detailed recording of methods, results, data analysis, and conclusions Show less • Provided target validation support and biomarker identification by planning, designing, and performing many studies for testing hypotheses on mechanism of action of lead oncology compounds from discovery to proof-of -concept stages, evaluated the compound’s effect on a variety of cell signaling pathway markers in both solid and hematological malignancies • Evaluated the potential anti-angiogenesis effect of the compounds using endothelial tube formation assay • Optimizing and… Show more • Provided target validation support and biomarker identification by planning, designing, and performing many studies for testing hypotheses on mechanism of action of lead oncology compounds from discovery to proof-of -concept stages, evaluated the compound’s effect on a variety of cell signaling pathway markers in both solid and hematological malignancies • Evaluated the potential anti-angiogenesis effect of the compounds using endothelial tube formation assay • Optimizing and implementing a Plasma Inhibitory Activity assay for PD biomarker changes in Acute Myeloid Leukemia (AML) and DLBCL cell lines to determine the inhibitor activity n plasma isolated from mice dosed with lead compound • Generated and examined drug-resistant cell line, established stable cell line by transfected lentivirus • Worked with CROs/vendors to generate custom cDNA. Developed the qRT-PCR assay for measuring splice-variant specific transcripts which included determination the long/-short mRNA ratio, optimizing several steps of the RNA extraction, and cDNA synthesis, developing primer-probe sets • Developed analysis PBMC samples from clinical trial to support biomarker development and provided reproducible results to facilitate clinical biomarker studies • Developed and provided the analysis plasma samples from clinical trial for Cytokine/Chemokine Multiplex Assay to guide the clinical biomarker studies • Provided key data was presented at 2015 AACR and 2017 ASH annual meetings • Participated in the data analysis and interpretation, writing reports, and presenting the data to upper management • Maintained of electronic laboratory notebook, including accurate and detailed recording of methods, results, data analysis, and conclusions Show less



Boston University School of Medicine

• United States
• Higher Education
• 700 & Above Employee

• Research Associate

  • Aug 2002 - Nov 2007

  - Initiated and developed numerous studies to understand mechanism of action of the effect of oligonucleotide on eumelanogenesis, hair pigmentation and melanocortin-1 receptor expression in the mouse hair follicle. - Maintained primary human cells for studying molecular characterization of BMP target genes. - Carried out tissue harvesting/processing, immunohistochemistry, in situ hybridization, TUNEL Assay, Western Blot Analysis, qRT-PCR. - Initiated and developed numerous studies to understand mechanism of action of the effect of oligonucleotide on eumelanogenesis, hair pigmentation and melanocortin-1 receptor expression in the mouse hair follicle. - Maintained primary human cells for studying molecular characterization of BMP target genes. - Carried out tissue harvesting/processing, immunohistochemistry, in situ hybridization, TUNEL Assay, Western Blot Analysis, qRT-PCR.