Experience

Izmir Institute of Technology

• Türkiye
• Higher Education
• 400 - 500 Employee

• Project Leader

  • Jan 2022 - Present

  Investigation of the Role of Prostaglandin E2 in Primary Breast Cancer Induced Lung Pre-metastatic Niche Formation via Microfluidic Chips

• Research Fellow

  • Jul 2017 - Present

  - Microfluidic platforms (lab on a chip), 3D modeling, 3D microenvironment, Organoids- Cancer metastasis assays in microfluidic platforms (invasion, extravasation, determining of pre-metastatic niches)- Confocal microscopy and Fluorescence microscopy techniques- Immunofluorescence staining in microfluidic platforms - Cell viability assays (Cell death mechanisms, XTT, MTT Assay, Alamar Blue Testing)- Techniques for determining cell death (PI, DAPI, AnnexinV/PI Staining, Cell cycle detection)- Cell culture techniques (2D and 3D, Aseptic techniques, Passaging of primary cell cultures (Biosafety level 1 and 2), Cell counting methods, Cell freezing, Cell thawing)- RNA, DNA and Protein Isolation (Bradford and Lowry Methods) - PCR Techniques (RT-PCR, qRT-PCR)- Western Blot- SDS and Native Gel Electrophoresis- ELISA Show less



Ege University School of Medicine, Tulay Aktas Oncology Hospital R&D Department

• Scientific Researcher

  • 2008 - Oct 2016

  -Executing and planning scientific projects, creating timelines, work packages based on desired deadlines -Performing lab tests (Cell viability assays, Cell death mechanisms, XTT, MTT Assay, Alamar Blue Testing, Techniques for determining cell death (PI, DAPI, AnnexinV/PI Staining, Cell cycle detection) -Mammalian cell culture techniques (2D and 3D, Aseptic techniques, Passaging of primary cell cultures (Biosafety level 1 and 2), Cell counting methods, Cell freezing, Cell thawing) -Drug testing/discovery and screening -xCELLigence Real-Time Cell Analysis -RNA, DNA and Protein Isolation (Bradford and Lowry Methods) -PCR Techniques (RT-PCR, qRT-PCR), Protein and PCR Array -Western Blot, SDS and Native Gel Electrophoresis, ELISA -Scratch Assay, Colony Formation Assay, -Data analysis using GraphPad Prism Show less