Ashley Spreen

Associate Scientist at Altis Biosystems
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Contact Information
us****@****om
(386) 825-5501
Location
College Station, Texas, United States, US

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Experience

    • United States
    • Biotechnology
    • 1 - 100 Employee
    • Associate Scientist
      • Feb 2022 - Present

    • Research Associate
      • Oct 2021 - Jan 2022

    • United States
    • Pharmaceutical Manufacturing
    • 700 & Above Employee
    • Scientist
      • Aug 2020 - Oct 2021

      -Provide technical stewardship for primary packaging components for the commercial parenteral platform. -Lead container-closure and market product complaint investigations through facilitating shared learning between parenteral manufacturing, device manufacturing, and vendors. -Manage cross functional project teams to implement continuous improvement projects. -Provide technical stewardship for primary packaging components for the commercial parenteral platform. -Lead container-closure and market product complaint investigations through facilitating shared learning between parenteral manufacturing, device manufacturing, and vendors. -Manage cross functional project teams to implement continuous improvement projects.

    • United States
    • Pharmaceutical Manufacturing
    • 700 & Above Employee
    • Manufacturing and Quality Intern
      • May 2019 - Aug 2019

      I led a cross functional project within manufacturing and process development looking to improve mixing for parenteral drug products and buffers in single use bags. I designed and built an In-Line Refractometer to continuously sample multiple sections of a mixing vessel to determine homogeneity of the solution in real time, which can be used to set proven acceptable ranges for every molecule. I characterized the minimum volume and mixing speed ranges for different volumes in a 1000L mixing… Show more I led a cross functional project within manufacturing and process development looking to improve mixing for parenteral drug products and buffers in single use bags. I designed and built an In-Line Refractometer to continuously sample multiple sections of a mixing vessel to determine homogeneity of the solution in real time, which can be used to set proven acceptable ranges for every molecule. I characterized the minimum volume and mixing speed ranges for different volumes in a 1000L mixing vessel to increase flexibility of the vessel as batch requirements change. I designed and built a probe to quantitate velocity in different regions of a cubic mixing vessel through integrating a strain gauge and Wheatstone bridge onto a probe. I also wrote studies and technical reports in accordance with cGMPs and GDPs. Show less I led a cross functional project within manufacturing and process development looking to improve mixing for parenteral drug products and buffers in single use bags. I designed and built an In-Line Refractometer to continuously sample multiple sections of a mixing vessel to determine homogeneity of the solution in real time, which can be used to set proven acceptable ranges for every molecule. I characterized the minimum volume and mixing speed ranges for different volumes in a 1000L mixing… Show more I led a cross functional project within manufacturing and process development looking to improve mixing for parenteral drug products and buffers in single use bags. I designed and built an In-Line Refractometer to continuously sample multiple sections of a mixing vessel to determine homogeneity of the solution in real time, which can be used to set proven acceptable ranges for every molecule. I characterized the minimum volume and mixing speed ranges for different volumes in a 1000L mixing vessel to increase flexibility of the vessel as batch requirements change. I designed and built a probe to quantitate velocity in different regions of a cubic mixing vessel through integrating a strain gauge and Wheatstone bridge onto a probe. I also wrote studies and technical reports in accordance with cGMPs and GDPs. Show less

    • Undergraduate Researcher
      • Aug 2017 - Dec 2018

      Undergraduate researcher at Texas A&M University, working with an engineering PhD candidate to investigate the biochemical effects of mechanical forces at the cellular level and their contributions to the development of lymphatic diseases. This is being achieved through the design and build of a bioreactor to expose lymphatic endothelial cells to fluid shear stress and stretch simultaneously. Undergraduate researcher at Texas A&M University, working with an engineering PhD candidate to investigate the biochemical effects of mechanical forces at the cellular level and their contributions to the development of lymphatic diseases. This is being achieved through the design and build of a bioreactor to expose lymphatic endothelial cells to fluid shear stress and stretch simultaneously.

    • SMART Intern
      • May 2018 - Aug 2018

      I worked on two ocular research projects at Texas Children's Hospital and Baylor College of Medicine. On one project, I worked on a novel ocular drug delivery system. I manufactured hydrogels with nanoreservoirs for drug loading. The hydrogels looked and felt like a contact lens, but slowly dissolved while releasing the loaded drug. For the second project, I worked to determine if the carboxy-terminal G3 domain of the protein versican, found in the vitreous of the eye, increases… Show more I worked on two ocular research projects at Texas Children's Hospital and Baylor College of Medicine. On one project, I worked on a novel ocular drug delivery system. I manufactured hydrogels with nanoreservoirs for drug loading. The hydrogels looked and felt like a contact lens, but slowly dissolved while releasing the loaded drug. For the second project, I worked to determine if the carboxy-terminal G3 domain of the protein versican, found in the vitreous of the eye, increases transgene expression in normal and cancer cells. This has potential impacts on transgene expression of suicide gene therapy to treat patients with retinoblastoma. Show less I worked on two ocular research projects at Texas Children's Hospital and Baylor College of Medicine. On one project, I worked on a novel ocular drug delivery system. I manufactured hydrogels with nanoreservoirs for drug loading. The hydrogels looked and felt like a contact lens, but slowly dissolved while releasing the loaded drug. For the second project, I worked to determine if the carboxy-terminal G3 domain of the protein versican, found in the vitreous of the eye, increases… Show more I worked on two ocular research projects at Texas Children's Hospital and Baylor College of Medicine. On one project, I worked on a novel ocular drug delivery system. I manufactured hydrogels with nanoreservoirs for drug loading. The hydrogels looked and felt like a contact lens, but slowly dissolved while releasing the loaded drug. For the second project, I worked to determine if the carboxy-terminal G3 domain of the protein versican, found in the vitreous of the eye, increases transgene expression in normal and cancer cells. This has potential impacts on transgene expression of suicide gene therapy to treat patients with retinoblastoma. Show less

Education

  • Texas A&M University
    Bachelor's degree, Biomedical Engineering
    2016 - 2020

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