Ashis Kumar
Senior Research Fellow at Centre for Stem Cell Research ,Department of Biotechnology,Christian Medical College- Claim this Profile
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Experience
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Centre for Stem Cell Research ,Department of Biotechnology,Christian Medical College
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India
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Higher Education
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1 - 100 Employee
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Senior Research Fellow
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Sep 2017 - Present
Regeneration of musculoskeletal stem cells using cell free therapy Finding a Novel method for seeding biomaterial scaffold Genetic analysis of rare inherited disorders of bone and cartilage using NGS Experienced in Bioinformatics workflow for genomics Regeneration of musculoskeletal stem cells using cell free therapy Finding a Novel method for seeding biomaterial scaffold Genetic analysis of rare inherited disorders of bone and cartilage using NGS Experienced in Bioinformatics workflow for genomics
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Centre for Cellular and Molecular Biology
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Research Services
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300 - 400 Employee
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Junior Research Fellow
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Sep 2016 - Oct 2017
Isolation, and excision of mice tissues, and collection of semen from mice Isolation of RNA and synthesis of cDNA from mice tissues Gene expression analysis using RT-PCR Nanopore sequencing for the detection of transplice events Cell/ Tissue lysate preparation, and estimation of protein using Bradford and BCA assay Western blot to check for the presence of target protien Isolation, and excision of mice tissues, and collection of semen from mice Isolation of RNA and synthesis of cDNA from mice tissues Gene expression analysis using RT-PCR Nanopore sequencing for the detection of transplice events Cell/ Tissue lysate preparation, and estimation of protein using Bradford and BCA assay Western blot to check for the presence of target protien
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Project Student
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Dec 2015 - Jun 2016
DNA isolation from Blood sample and tissue Primer designing using NCBI PCR amplification of the desired exon Agarose Gel electrophoresis Sanger Sequencing using automated sequencer Sequence analysis using various Bioinfomatic tools Protein Homology modelling and Ab initio modelling using Schrödinger software Identification of protein functional properties in case of amino acid changes DNA isolation from Blood sample and tissue Primer designing using NCBI PCR amplification of the desired exon Agarose Gel electrophoresis Sanger Sequencing using automated sequencer Sequence analysis using various Bioinfomatic tools Protein Homology modelling and Ab initio modelling using Schrödinger software Identification of protein functional properties in case of amino acid changes
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Christian medical college and hospital
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India
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Education Administration Programs
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700 & Above Employee
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Graduate medical laboratory technologist
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Jan 2011 - Jan 2014
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Education
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Loyola College
Master’s Degree, Biotechnology -
CHRISTIAN MEDICAL COLLEGE AND HOSPITAL, COLLEGE OF NURSING (Inst. Code - 033), VELLORE
Bachelor of science in Medical Laboratory Technology, Diagnostic Laboratory techniques