Experience

Rajiv Gandhi Centre For Biotechnology (RGCB)

• India
• Biotechnology Research
• 100 - 200 Employee

• Post Doctoral Tutor

  • May 2020 - Present

  Tutor for MSc Biotechnology course at Rajiv Gandhi Center for BiotechnologySubjects Taught: Microbiology, Disease Biology, Molecular Diagnostics, Research MethodologyLabs: Microbiology Labs, Genetic Engineering Lab

• Post Doctoral Research Associate in Pathogen Biology lab

  • Aug 2018 - May 2020

  • Deciphering the effect of maternal postpartum antibiotic prophylaxis on infant gut microbiome and its resistance development• Metataxonomic analysis of bacterial diversity and its antibiotic profiling of environmental biotopes• Studied the benefits of probiotics and explored its therapeutic interventions in gastric disorders



University of Edinburgh

• Kings Buildings, Ediburgh

• PhD student and Teaching Assistant in Biological Sciences

  • Oct 2011 - Jul 2017

  Studied binding and degradation of host proteins by Bacteroides fragilis, a commensal bacterium residing in the human GI tract The research provided insights into the nutritional requirements and infectious nature of the opportunistic pathogen, Bacteroides fragilis Investigated effects of nitrogen limitation on its transcriptome Maintained and recorded strains for future experimental purposes Managed inventory and replenishment of required lab reagents and other equipment needed for the efficient functioning of the lab Analyzed quality of read pair sequences using FastQC tool and aligned reads against reference genome using Bowtie tool Quantified and Identified diferentially expressed genes using HTSeq and DESeq tools Annotated prokaryotic genomes using RAST server (UG lab sessions) Designed Primers efficiently and cost effectively for experiments using Net Primer and Artermis genome browser Constructed gene deletion mutants in Bacteroides fragilis using a markerless gene deletion method developed in our lab Functionally analysed the presence of bacterial proteases using substrate zymography Conducted bacterial protein expression and purification studies to analyse their interactions with the host proteins Presented doctoral research work at local, national and international levels (seminars, poster presentations) Received scholarships from the University and The Microbiology Society (formerly SGM) for poster presentation at The Anaerobe 2014, Chicago, United States (July, 2014) Presented doctoral work at The Microbiology Society Autumn Conference, University of Sussex (September, 2013) Poster presentation and seminar as part of the Ph.D. curriculum (December, 2013) Successful completion of annual reviews Show less



Institute of Genomics and Integrative Biology, CSIR, Delhi

• New Delhi Area, India

• Research Associate

  • Sep 2010 - Sep 2011

  Performed molecular characterisation of the role of metal ions in mycobacteria. Worked in a BSL3 facility lab. Studied the possible involvement of ctpA gene in copper export and detoxification of mycobacterial cells. Successfully cloned ctpA gene in M.smegmatis and proceeded with testing copper tolerance in liquid cultures. Attended a conference in Delhi University (South Campus) on de novo sequencing techniques. Performed molecular characterisation of the role of metal ions in mycobacteria. Worked in a BSL3 facility lab. Studied the possible involvement of ctpA gene in copper export and detoxification of mycobacterial cells. Successfully cloned ctpA gene in M.smegmatis and proceeded with testing copper tolerance in liquid cultures. Attended a conference in Delhi University (South Campus) on de novo sequencing techniques.