Experience

Indian Institute of Science Education and Research, Thiruvananthapuram

• Thiruvananthapuram Area, India

• PHD Graduate Student SRF

  • Aug 2016 - Present

  Understand the role of autophagy via protein complex formation in host defense: •explore the area of host-pathogen interaction using macrophages •understand the role of protein complexes formed upon TLR signaling primed by LPS, E. coli or Mycobacterium smegmatis •analyze the structural organization of these complexes using super-resolution microscopy •identify proteins recruited into these complexes by mass spectrometry •large data analyses to focus on defense response proteins identified by MS • analyze and validate the presence of these proteins as functional constituents of the protein complexes



Indian Institute of Science (IISc)

• Bengaluru, Karnataka, India

• Research Collaborator

  • Jun 2022 - Sep 2022

  Performed experiments to establish the role of protein complexes against bacterial survival, formed in macrophages in response to infection by Salmonella, Mycobacterium, E. coli, etc. Performed experiments to establish the role of protein complexes against bacterial survival, formed in macrophages in response to infection by Salmonella, Mycobacterium, E. coli, etc.



Indian Institute of Technology, Madras

• Chennai, Tamil Nadu, India

• Research Collaborator

  • Jul 2016 - Aug 2016

  Worked with 3D super-resolution structured illumination microscopy to elucidate the structural organization of protein complexes. Worked with 3D super-resolution structured illumination microscopy to elucidate the structural organization of protein complexes.



ICGEB

• New Delhi, Delhi, India

• Junior Research Fellow

  • Jul 2013 - Jul 2014

  Identify novel vaccine candidates against the Malarial parasite, Plasmodium falciparum: •study of novel molecules from Plasmodium that can be targeted for vaccine development •study important pathways involved in the invasion of erythrocytes by P. falciparum •identify smaller recombinant protein constructs from the merozoite invasin of the Plasmodium falciparum Reticulocyte binding protein homologue (PfRH4) that would be important for their affinity towards erythrocytic receptor CR1 •raised polyclonal antibodies against the constructs in mice and rabbits to evaluate invasion inhibitory activity, their minimal binding affinity and their potential to induce neutralizing antibody response



Jawaharlal Nehru University, SLS

• New Delhi Area, India

• Master Thesis Project

  • Jul 2012 - May 2013

  Cloning, expression and purification of BAR domain-containing proteins in Entamoeba histolytica: •understand molecular interactions in erythrocyte phagocytosis by Entamoeba histolytica •study membrane modulators involved in phagocytosis •identify BAR (Bin–Amphiphysin–Rvs) domain-containing proteins expressed in E. histolytica through bioinformatic analyses •cloning, over-expression and purification of 3 novel BAR domain-containing proteins of Entamoeba histolytica for further analyses of their role in erythrocyte phagocytosis



Delhi University, DPMB

• New Delhi, Delhi, India

• Summer Intern

  • May 2010 - Jul 2010

  Analysis of Protein Interaction of Arabidopsis thaliana CIPKs with PP2C Protein using Yeast­2­Hybrid System: •study the calcium dependant plant defense mechanisms under abiotic stress •understand the roles and possible interactions between protein phosphatases and CBL interacting proteins •use the yeast-2-hybrid method to detect interactions. HIS reporter gene expression and filter lift assay to confirm the observed interactions Analysis of Protein Interaction of Arabidopsis thaliana CIPKs with PP2C Protein using Yeast­2­Hybrid System: •study the calcium dependant plant defense mechanisms under abiotic stress •understand the roles and possible interactions between protein phosphatases and CBL interacting proteins •use the yeast-2-hybrid method to detect interactions. HIS reporter gene expression and filter lift assay to confirm the observed interactions