Experience

The Martin Luther University of Halle-Wittenberg

• Germany
• Higher Education
• 400 - 500 Employee

• Postdoctoral Fellow

  • Jul 2014 - Present

  Here at the Institute for Physiological Chemistry, I am working on the generation and characterization of a new constitutively active mouse model for a transcriptional cofactor MRTF-A. MRTF-A acts a cofactor for SRF, thereby regulating a subset of the genes regulated by SRF. We believe that this mouse model would be proven useful in the study of tissue fibrosis of various organ as well as in the study of cancer metastasis. Here at the Institute for Physiological Chemistry, I am working on the generation and characterization of a new constitutively active mouse model for a transcriptional cofactor MRTF-A. MRTF-A acts a cofactor for SRF, thereby regulating a subset of the genes regulated by SRF. We believe that this mouse model would be proven useful in the study of tissue fibrosis of various organ as well as in the study of cancer metastasis.



Hannover Medical School

• Germany
• Medical Practices
• 500 - 600 Employee

• Post Doctoral Fellow

  • Jun 2008 - Dec 2013

  As a post doctoral fellow I continued to work in the gastrointestinal physiology and developed in vivo methods to measure different physiological parameters. I tried to adopt and refine published in vivo methods to study transport across the gastrointestinal epithelium, as close as to the physiological situations. I measured bicarbonate and fluid transport in luminally perfused mice that were defective for ion transporters, the NHERF proteins, or for other regulatory proteins such as kinases… Show more As a post doctoral fellow I continued to work in the gastrointestinal physiology and developed in vivo methods to measure different physiological parameters. I tried to adopt and refine published in vivo methods to study transport across the gastrointestinal epithelium, as close as to the physiological situations. I measured bicarbonate and fluid transport in luminally perfused mice that were defective for ion transporters, the NHERF proteins, or for other regulatory proteins such as kinases, enzymes or receptors, under a variety of challenging experimental conditions. I also develop the fluorimetric method to assess intra as well as luminal surface pH in different segments of gastrointestinal tract using two-photon microscope in vivo. During this time I also standardized many immunohistochemistry protocols as well as methods to assess the mucus secretion in vivo.

• PhD

  • Oct 2004 - Jun 2008

  Dissertation Title: "Duodenal mucosal bicarbonate secretion: role of CFTR, Slc26a6, carbonic anhydrase (II) and NHERF family adapter proteins in health an disease" During my PhD I established many techniques in the lab, collaborated and co-authored several conferences and international papers with researchers in different areas of gastrointestinal physiology, cell and molecular biology and cancer biology. My doctoral dissertation was conducted in the area of the duodenal mucosal… Show more Dissertation Title: "Duodenal mucosal bicarbonate secretion: role of CFTR, Slc26a6, carbonic anhydrase (II) and NHERF family adapter proteins in health an disease" During my PhD I established many techniques in the lab, collaborated and co-authored several conferences and international papers with researchers in different areas of gastrointestinal physiology, cell and molecular biology and cancer biology. My doctoral dissertation was conducted in the area of the duodenal mucosal bicarbonate secretion, electrolyte and fluid balance in relation to inflammatory bowl disease mouse models and mouse models for other disease such as cystic fibrosis. My recent research focused on the validation of formation of macromolecular signaling complexes in vivo with particular interest in cystic fibrosis, which will eventually lead to better understand the disease and therapeutic interventions. The development of in vivo setup for the measurement of various physiological parameters and in vivo two photon microscopy for the measurement of intra and extracellular pH, intracellular Ca2+ and mucus secretion measurements in the gut are major highlights of my research work so far.



Hindustan Lever Research Centre

• White Field, Bengaluru, Karnataka, India

• Summer Trainee

  • May 2003 - Jul 2003

  I developed method to measure melanin pigment in different skin samples. The project involved enzymatic degradation of melanin followed by thin layer chromatographic separation of the products and estimation of melanin content using LC-MS. I developed method to measure melanin pigment in different skin samples. The project involved enzymatic degradation of melanin followed by thin layer chromatographic separation of the products and estimation of melanin content using LC-MS.