Experience

Eigen Therapeutics
• United States
• Biotechnology Research
• 1 - 100 Employee
• Senior Scientist

  • Sep 2022 - Present


Notable

• United States
• Advertising Services
• 1 - 100 Employee

• Senior Scientist

  • May 2021 - Aug 2022

• Scientist

  • May 2019 - Jun 2021



Abbvie Stemcentrx LLC

• Scientist I

  • Jan 2015 - May 2019

  • Maintenance and characterization of disseminated AML PDX bank, target mining and identification, target validation • Discovered and developed a novel serum biomarker for efficacy assessment in AML PDX models • Screened novel linker drugs using cytotoxicity assays in AML cell lines. • Supported solid tumor indications by screening for heme tox concerns • Maintenance and characterization of disseminated AML PDX bank, target mining and identification, target validation • Discovered and developed a novel serum biomarker for efficacy assessment in AML PDX models • Screened novel linker drugs using cytotoxicity assays in AML cell lines. • Supported solid tumor indications by screening for heme tox concerns



Theravance

• Research Associate

  • Sep 2014 - Dec 2014

  • Detection and quantification of key protein expression levels with and without treatment with test compounds - Homogenized lung tissue and extracted protein lysates. - Ran ELISAs for detection of protein expression levels in the different study groups. - Analyzed data and presented the same at weekly group meetings • Cell based assay to monitor inhibitory drug potency - Cultured primary mouse alveolar epithelial cells. - Stimulated with cytokine of interest… Show more • Detection and quantification of key protein expression levels with and without treatment with test compounds - Homogenized lung tissue and extracted protein lysates. - Ran ELISAs for detection of protein expression levels in the different study groups. - Analyzed data and presented the same at weekly group meetings • Cell based assay to monitor inhibitory drug potency - Cultured primary mouse alveolar epithelial cells. - Stimulated with cytokine of interest and pre-treated with inhibitory drugs to check potency levels of different drug candidates. - Analyzed data and presented the same at weekly group meetings Show less • Detection and quantification of key protein expression levels with and without treatment with test compounds - Homogenized lung tissue and extracted protein lysates. - Ran ELISAs for detection of protein expression levels in the different study groups. - Analyzed data and presented the same at weekly group meetings • Cell based assay to monitor inhibitory drug potency - Cultured primary mouse alveolar epithelial cells. - Stimulated with cytokine of interest… Show more • Detection and quantification of key protein expression levels with and without treatment with test compounds - Homogenized lung tissue and extracted protein lysates. - Ran ELISAs for detection of protein expression levels in the different study groups. - Analyzed data and presented the same at weekly group meetings • Cell based assay to monitor inhibitory drug potency - Cultured primary mouse alveolar epithelial cells. - Stimulated with cytokine of interest and pre-treated with inhibitory drugs to check potency levels of different drug candidates. - Analyzed data and presented the same at weekly group meetings Show less



Plikus Lab

• Graduate Student Researcher

  • Sep 2013 - Sep 2014

  1) The Mouse Ear: A new model system to study hair cycle regulation: My main project during my time at the Plikus lab. I carried out: - performing procedures on mice under anaesthesia - tissue collection and imaging (Gross imaging and Fluorescent microscopy) - EdU pulse-chase experiments - EdU assay development and optimization 2) In-vivo hair characterization studies using mouse models - breeding and handling in vivo mouse models - sample collection… Show more 1) The Mouse Ear: A new model system to study hair cycle regulation: My main project during my time at the Plikus lab. I carried out: - performing procedures on mice under anaesthesia - tissue collection and imaging (Gross imaging and Fluorescent microscopy) - EdU pulse-chase experiments - EdU assay development and optimization 2) In-vivo hair characterization studies using mouse models - breeding and handling in vivo mouse models - sample collection and characterization - sample imaging and measurement - data collection and analysis - presentation of analyzed data - collaboration with the Mathematics department for mathematical modeling and statistical analysis 3) Optimization of dermal and epidermal cell collection from Mouse back skin This mini-project was carried out using a special LEF-RFP/K14-H2B-GFP mouse model > Mouse tissue collection (back skin) > Tissue processing, to obtain single cell suspension > FACS to separate out the dermal and epidermal components of skin 4) Trained undergraduate students in the essential techniques required for the above projects Show less 1) The Mouse Ear: A new model system to study hair cycle regulation: My main project during my time at the Plikus lab. I carried out: - performing procedures on mice under anaesthesia - tissue collection and imaging (Gross imaging and Fluorescent microscopy) - EdU pulse-chase experiments - EdU assay development and optimization 2) In-vivo hair characterization studies using mouse models - breeding and handling in vivo mouse models - sample collection… Show more 1) The Mouse Ear: A new model system to study hair cycle regulation: My main project during my time at the Plikus lab. I carried out: - performing procedures on mice under anaesthesia - tissue collection and imaging (Gross imaging and Fluorescent microscopy) - EdU pulse-chase experiments - EdU assay development and optimization 2) In-vivo hair characterization studies using mouse models - breeding and handling in vivo mouse models - sample collection and characterization - sample imaging and measurement - data collection and analysis - presentation of analyzed data - collaboration with the Mathematics department for mathematical modeling and statistical analysis 3) Optimization of dermal and epidermal cell collection from Mouse back skin This mini-project was carried out using a special LEF-RFP/K14-H2B-GFP mouse model > Mouse tissue collection (back skin) > Tissue processing, to obtain single cell suspension > FACS to separate out the dermal and epidermal components of skin 4) Trained undergraduate students in the essential techniques required for the above projects Show less



University of California, Irvine

• United States
• Higher Education
• 1 - 100 Employee

• Graduate Teaching Assistant

  • Sep 2013 - Jun 2014

  As a teaching assistant, I handled three different courses: • Two graduate level laboratory courses for Molecular Biology and Stem cell Biology • One undergraduate level lecture course on AIDS For the graduate level courses, I was responsible for: • Training students in fundamental techniques of Molecular Biology and Stem Cell Biology, as well as stem cell culture maintenance • Preparing and planning for the lab, setting up experiments, organizing schedules, preparing and… Show more As a teaching assistant, I handled three different courses: • Two graduate level laboratory courses for Molecular Biology and Stem cell Biology • One undergraduate level lecture course on AIDS For the graduate level courses, I was responsible for: • Training students in fundamental techniques of Molecular Biology and Stem Cell Biology, as well as stem cell culture maintenance • Preparing and planning for the lab, setting up experiments, organizing schedules, preparing and grading in-lab quizes and pre- and post- lab meetings • Coaching students on experimental data analysis, report writing and giving presentations Molecular biology: > DNA Digestion, Ligation > Plasmid vector preparation > DNA Gel Electrophoresis > Transformation > SDS-PAGE > Protein expression and purification (E. Coli) > HPLC (basics) > MS (basics) Stem cell culture maintenance: > mESC, hESC, iPSC > feeding, culturing, splitting, passaging > feeder and feeder-free culture methods > maintenance in undifferentiated state > differentiation > transfection, nucleofection > immunocytochemistry > imaging (bright-phase, fluorescent) For the Undergraduate course, I was responsible for: • holding office hours for the students • administering and grading tests Show less As a teaching assistant, I handled three different courses: • Two graduate level laboratory courses for Molecular Biology and Stem cell Biology • One undergraduate level lecture course on AIDS For the graduate level courses, I was responsible for: • Training students in fundamental techniques of Molecular Biology and Stem Cell Biology, as well as stem cell culture maintenance • Preparing and planning for the lab, setting up experiments, organizing schedules, preparing and… Show more As a teaching assistant, I handled three different courses: • Two graduate level laboratory courses for Molecular Biology and Stem cell Biology • One undergraduate level lecture course on AIDS For the graduate level courses, I was responsible for: • Training students in fundamental techniques of Molecular Biology and Stem Cell Biology, as well as stem cell culture maintenance • Preparing and planning for the lab, setting up experiments, organizing schedules, preparing and grading in-lab quizes and pre- and post- lab meetings • Coaching students on experimental data analysis, report writing and giving presentations Molecular biology: > DNA Digestion, Ligation > Plasmid vector preparation > DNA Gel Electrophoresis > Transformation > SDS-PAGE > Protein expression and purification (E. Coli) > HPLC (basics) > MS (basics) Stem cell culture maintenance: > mESC, hESC, iPSC > feeding, culturing, splitting, passaging > feeder and feeder-free culture methods > maintenance in undifferentiated state > differentiation > transfection, nucleofection > immunocytochemistry > imaging (bright-phase, fluorescent) For the Undergraduate course, I was responsible for: • holding office hours for the students • administering and grading tests Show less



Allergan

• Pharmaceutical Manufacturing
• 700 & Above Employee

• Research Intern

  • Jun 2013 - Sep 2013

  During my time at Allergan, I was mainly involved in the following three projects: 1) Gene Expression Studies Testing Novel Ophthalmic Compound - Cell Culture of primary human RPE cells and stable transformed RPE cell line (RPE-19) - RNA extraction and cDNA preparation from cells treated with compound - qPCR to measure gene expression of cAMP responsive genes - qPCR data analysis and interpretation 2) In vivo Experiments Using Wild-Type and… Show more During my time at Allergan, I was mainly involved in the following three projects: 1) Gene Expression Studies Testing Novel Ophthalmic Compound - Cell Culture of primary human RPE cells and stable transformed RPE cell line (RPE-19) - RNA extraction and cDNA preparation from cells treated with compound - qPCR to measure gene expression of cAMP responsive genes - qPCR data analysis and interpretation 2) In vivo Experiments Using Wild-Type and Knockout Mice Testing Compounds For Photoreceptor Protection - Mouse model using bright light to induce photoreceptor damage - Measured damage induced by light both visually by Heildelberg Retinal Angiograph (HRA) for auto-fluorescence and Electro-Retinal Angiogram (ERG) for functional response. - Whole eye enucleation for histological analysis, posterior eye-cup preparation and retina dissection for other studies. 3) Performed experiments Testing Novel Dermatology Drug - In vivo drug testing using Golden Syrian Hamsters for effect on the sebaceous gland - Prepared drug prior to treatment - Quantitative measurement of dermal thickness response after drug treatment Presented the results and progress of these projects to the Vice-Presidents of Allergan and other team members at the monthly meeting Show less During my time at Allergan, I was mainly involved in the following three projects: 1) Gene Expression Studies Testing Novel Ophthalmic Compound - Cell Culture of primary human RPE cells and stable transformed RPE cell line (RPE-19) - RNA extraction and cDNA preparation from cells treated with compound - qPCR to measure gene expression of cAMP responsive genes - qPCR data analysis and interpretation 2) In vivo Experiments Using Wild-Type and… Show more During my time at Allergan, I was mainly involved in the following three projects: 1) Gene Expression Studies Testing Novel Ophthalmic Compound - Cell Culture of primary human RPE cells and stable transformed RPE cell line (RPE-19) - RNA extraction and cDNA preparation from cells treated with compound - qPCR to measure gene expression of cAMP responsive genes - qPCR data analysis and interpretation 2) In vivo Experiments Using Wild-Type and Knockout Mice Testing Compounds For Photoreceptor Protection - Mouse model using bright light to induce photoreceptor damage - Measured damage induced by light both visually by Heildelberg Retinal Angiograph (HRA) for auto-fluorescence and Electro-Retinal Angiogram (ERG) for functional response. - Whole eye enucleation for histological analysis, posterior eye-cup preparation and retina dissection for other studies. 3) Performed experiments Testing Novel Dermatology Drug - In vivo drug testing using Golden Syrian Hamsters for effect on the sebaceous gland - Prepared drug prior to treatment - Quantitative measurement of dermal thickness response after drug treatment Presented the results and progress of these projects to the Vice-Presidents of Allergan and other team members at the monthly meeting Show less



In Vitro International Pvt. Ltd., Bangalore

• Summer Intern

  • Jun 2011 - Aug 2011

  • Trained in and carried out the techniques of micropropagation: > Plant media preparation > Culture Initiation > Culture Establishment > Culture Multiplication > Culture Packaging for export • Trained in rigorous aseptic technique to prevent contamination • Trained in and carried out the techniques of micropropagation: > Plant media preparation > Culture Initiation > Culture Establishment > Culture Multiplication > Culture Packaging for export • Trained in rigorous aseptic technique to prevent contamination



Millipore, India

• Biotechnology Research
• 1 - 100 Employee

• R&D and Process Laboratory Intern

  • Jun 2010 - Jul 2010

  Trained in > understanding SOPs > Environmental monitoring > Clean room etiquette > Filter integrity testing (basics) > HPLC and its application in Extractable Analysis > Filtration(using the various Millipore equipment) > Protein estimation using BCA > SDS PAGE > Luminex (multi-ELISA technology) Trained in > understanding SOPs > Environmental monitoring > Clean room etiquette > Filter integrity testing (basics) > HPLC and its application in Extractable Analysis > Filtration(using the various Millipore equipment) > Protein estimation using BCA > SDS PAGE > Luminex (multi-ELISA technology)



PES Institute of Technology, Bangalore

• Student Assistant

  • 2010 - 2010

  • Assisted in teaching Bioinformatics • Prepared notes and study material for the class • Assisted in teaching Bioinformatics • Prepared notes and study material for the class