Anthony Bayega
Research Associate at McGill Genome Centre- Claim this Profile
Click to upgrade to our gold package
for the full feature experience.
Topline Score
Bio
Credentials
-
Duolingo French Fluency: Elementary (Estimated)
DuolingoAug, 2016- Oct, 2024 -
Duolingo French Fluency: Beginner (Estimated)
DuolingoMay, 2016- Oct, 2024
Experience
-
McGill Genome Centre
-
Canada
-
Research
-
1 - 100 Employee
-
Research Associate
-
Apr 2021 - Present
Main responsibilities: • Evaluate commercially available SARS-CoV-2 whole genome amplification protocols to assess their performance in response to the COVID19 pandemic. • Develop a very sensitive method for SARS-CoV-2 whole genome amplification and sequencing suitable for low viral titre samples such as waste water samples. • Evaluate commercially available methods and/or protocols for generating next generation sequencing (NGS) libraries to assess their suitability and performance. • Optimise commercially available NGS library preparation protocols to ensure optimum performance in the lab. • Design new methods to generate improved protocols for preparing NGS libraries. • Apply new technologies for genome assembly and characterization, such as linked read technology, HiC based genome assembly and long read NGS technology. • Generate data using third generation NGS platforms (PacBio, Nanopore) and perform multiplatform integrated analysis. • Analyze genomic datasets using bioinformatics tools and computer programming in Python, Bash, and R. • Prepare scientific manuscripts for submission to pear-reviewed journals. • Develop training materials for the analytical pipelines suitable for more junior staff and student • Present scientific findings both in lab meetings and to the general public and scientific meetings. • Troubleshoot analysis pipeline problems as necessary to ensure operational integrity. Major achievements • Developed the Panhandle protocol for dramatically improving long-read RNA sequencing data quality. • Developed the ARTIC-Amp protocol for increased SARS-CoV2 whole genome sequencing sensitivity. Show less
-
-
-
McGill University
-
Canada
-
Higher Education
-
700 & Above Employee
-
Teaching Assistant
-
Jan 2018 - May 2021
I carried out Teaching Assistant (TA) roles for 180 hours per semester for a total of 1260 hours over 7 semesters. The course was titled "BIOL 301 – Cell and Molecular Laboratory" in the Department of Biology, Faculty of Science, McGill University. TA roles included the following. • Lead one six-hour molecular biology hands-on practical per week comprising 40 - 50 students. • Prepare pre-lab talk in rotation with other demonstrators. • Hold tutorial every 3rd or 4th week in rotation with other demonstrators. • Grade 14 lab reports each week and grade final exams. • Participate in pre-lab orientation (45 minutes/week) and weekly TA meetings. • Hold one two-hour review session prior to final exam. • Assist in course development. Show less
-
-
-
Wellcome Genome Campus
-
United Kingdom
-
Wellness and Fitness Services
-
1 - 100 Employee
-
Teaching Assistant
-
Jun 2019 - Jun 2019
I carried out Teaching Assitant (TA) roles for the RNA Transcriptomics Advanced Course conducted at the Welcome Genome Campus, Hinxton, Cambridge, UK CB10 1SA. TA roles included the following. • Develop protocol for the lab experiments. • Develop protocol for computational analysis of lab data. • Lead students through lab experiments and data analysis. The subjects covered included the following. * Overview of single molecule long read sequencing technologies * Full length cDNA sequencing * Small RNA sequencing * Single cell transcriptome data generation and analysis * Identification of post-transcriptional modifications in RNA (epi-transcriptomics) * Functional assays for lncRNA biology * Integration of microRNA and mRNA data; functional analysis of microRNAs * Expression network and pathway integration and analysis Show less
-
-
-
Mildmay Uganda
-
Uganda
-
Hospitals and Health Care
-
300 - 400 Employee
-
Laboratory Technologist
-
Nov 2015 - Jan 2016
Main responsibilities: • Drew blood samples by venipuncture (phlebotomy) and received samples delivered by patients. • Processed samples in the routine clinical diagnostics lab (hematology, serology, chemistry). • Assisted in developing standard operating procedures. Main responsibilities: • Drew blood samples by venipuncture (phlebotomy) and received samples delivered by patients. • Processed samples in the routine clinical diagnostics lab (hematology, serology, chemistry). • Assisted in developing standard operating procedures.
-
-
-
-
Postgraduate trainee
-
Jan 2013 - Aug 2014
• Maintain cell Human Hepatoma (Huh) cell line and other cell lines as required by work. • Perform Hepatitis C virus (HCV) culture in suitable cell line(s). • Harvest, purify, and quantify Hepatitis C virus cultures for use in lab experiments. • Perform advanced molecular experiments to evaluate in-vitro and mouse virus-host relationships. • Clone HCV genes in plasmid and viral vectors. • Produce and purify HCV proteins in E. coli system • Immunise mice with purified and adjuvanted proteins to generate antibodies • Collect animal spleen and generate monoclonal antibodies using mouse Hybridoma technology • Characterize the monoclonal antibodies to determine their recognition sites • Perform other advanced molecular biology lab techniques such as ELISA, mouse husbandry, Western blot, qRT-PCR, e.t.c as required to perform molecular virology. Position highlight Purified and characterized a commercially available mouse monoclonal antibody against Hepatitis C virus. Show less
-
-
-
UVRI-IAVI HIV VACCINE PROGRAM
-
Uganda
-
Non-profit Organizations
-
1 - 100 Employee
-
Laboratory Technologist
-
Nov 2008 - Aug 2011
During my employment at Uganda Virus Research Institute – International AIDS Vaccine Initiative, I accomplished the following. • I received samples from all these projects requiring a high level of attention to detail to ensure sample IDs and accompanying requisition forms IDs matched and that the appropriate number of tubes, sample volume, and correct sample container/tube was used to deliver the sample and that samples were delivered in the correct conditions such as temperature and integrity. • Processed the samples following the respective SOPs. Biochemical and serological assays included liver function tests, renal function test, PBMC isolation using Ficoll, full blood count, ELISpot assay, rapid HIV antigen and antibody tests, CD4 and CD8 counting among other tests. • Entered resulting test results and samples such as PBMCs, serum, plasma into LIMS and generated detailed manifests to aid in proper storage and archiving of samples. Further, I ensured that all data generated in the lab was regularly backed up in LIMS and safely stored. • Stored all required samples in the respective repositories such as -20 freezers, -80 freezers, and liquid nitrogen freezers according to GCLP principles and retrieved and prepared required samples for international shipment following IATA guidelines. • Ensured that there was adequate liquid nitrogen supply to the cryostorage tanks and was directly responsible for initiating and receiving supplies of liquid nitrogen. • Ensured that data generated by the fridge and freezer temperature monitoring system was downloaded, printed, and stored, and also manually recorded freezer temperatures when the system was not functional or for the fridges that did not have remove temperature monitoring systems. • Participated in the review, update and writing of SOPs whenever necessary after approval by the senior scientist and also perform and evaluated daily protocol and/or equipment quality controls in accordance with standards. Show less
-
-
-
-
Medical Laboratory Technician
-
Jul 2008 - Oct 2008
Mengo hospital Laboratory is a very busy routine clinical diagnostics lab. I routinely carried out the following tasks. • Perform Phlebotomy to collect clinical samples. • Process samples in the routine clinical diagnostics labs (hematology, microbiology, serology, chemistry). • Keeps accurate records of laboratory test results. • Provide comprehensive laboratory services to the clients and takes necessary steps to ensure that all clients are treated with respect and dignity • Ensure that adequate stocks of supplies and reagents are maintained. • Be able to conduct and report on the tests based on laboratory request form and recorded client notes. • Ensure that the highest standards of laboratory services are provided to clients as per MOH guidelines. • Ensure that the laboratory complies with Infection Prevention Guidelines. • Ensure that laboratory test orders and results are properly recorded. • Ensure that timely monthly laboratory reports are forwarded to the Laboratory Manager. • Perform preventive maintenance of all equipment and ensure that their timely calibration and servicing. • Ensure that costs in the laboratory are contained within the approved annual budget, and that laboratory services meet targets planned. • Work closely with the Laboratory Manager towards the targets set by the organization. Show less
-
-
Education
-
2016 - 2021
McGill University
Human Genetics (PhD), Human Genetics -
2013 - 2014
National University of Singapore
Microbiology, Molecular Virology -
2011 - 2012
King's College London
Master of Science Biomedical and Molecular Sciences Research, Biomedical Sciences, General -
2005 - 2008
Makerere University
Bachelor of Biomedical Laboratory Technology, Biological and Biomedical Sciences -
2003 - 2004
St. Mary's College Kisubi
Uganda Advanced Certificate of Education, Sciences (Biology, Chemistry, Math, Agriculture)
Community
