Experience

Suomen Punainen Risti, Veripalvelu

• Finland
• Hospitals and Health Care
• 100 - 200 Employee

• Production Specialist

  • Jan 2020 - Present



Finnish Red Cross Blood Service

• Helsinki Area, Finland

• Project Reseacher

  • May 2015 - Dec 2019

  Working as a project researcher in the process development of immune cell therapies at the Advanced Cell Therapy Centre and R & D, Finnish Red Cross Blood Service, Helsinki, Finland. Three projects aiming to produce scientific publications for PhD thesis: - Establishment of a platform for in vitro production and characterization of Chimeric Antigen Receptor (CAR) T cells - Evaluation of starting materials for gene-modified immune cell therapies using CAR T-cells as an example product - Comparative analysis of lentiviral vector final formulation conditions for ex vivo transduction of T cells; a collaboration project with the Kuopio Center for Gene and Cell Therapy, Kuopio, Finland Methods used: - Expansion of primary human T cells from healthy and patient donors - Lentiviral and retroviral gene delivery - T-cell in vitro functionality assays - Cell phenotype analysis using Fluorescent Activated Cell Sorting (FACS) - Lentiviral vector particle and functional titer determination Show less



University of Jyväskylä

• Finland
• Higher Education
• 700 & Above Employee

• MSc Thesis project

  • Feb 2014 - Aug 2014

  Studying the role of Human Parvovirus B19 Non-Structural Protein 1 in the development of autoimmune diseases. Methods included: - Primer designing, PCR, and cloning - Insect cell culture and the baculovirus expression vector system - Ni2+-affinity chromatography and protein gel filtration - Western blotting and ELISA Studying the role of Human Parvovirus B19 Non-Structural Protein 1 in the development of autoimmune diseases. Methods included: - Primer designing, PCR, and cloning - Insect cell culture and the baculovirus expression vector system - Ni2+-affinity chromatography and protein gel filtration - Western blotting and ELISA



University of Eastern Finland

• Finland
• Higher Education
• 700 & Above Employee

• Internship Trainee

  • May 2013 - Jul 2013

  Studying the roles of hyaluronan and hyaluronan synthase 2-gene in fibroblast migration, morphology, and responses induced by platelet-derived growth factor. Methods included: - Cell culture - Invasion assays and morphological assays: siRNA, immunofluorescence labeling, and confocal microscopy, immunohistochemical stainings - Western blotting and RT-qPCR Studying the roles of hyaluronan and hyaluronan synthase 2-gene in fibroblast migration, morphology, and responses induced by platelet-derived growth factor. Methods included: - Cell culture - Invasion assays and morphological assays: siRNA, immunofluorescence labeling, and confocal microscopy, immunohistochemical stainings - Western blotting and RT-qPCR