Experience

NIPD Genetics

• Cyprus
• Biotechnology Research
• 1 - 100 Employee

• Laboratory Technologist

  • Jun 2020 - Present



Department of Biosciences, University of Birmingham

• Birmingham, United Kingdom

• Msc Thesis

  • May 2018 - Jul 2018

  -An investigation of protein interactions during gene expression in eukaryotic cells- During my MSc Thesis i came across with RNA biology and particurarly with mRNA degradation. In vivo degradation of nonsense mRNA through nonsense-mediated decay (NMD) is one of the variables of mechanisms that cells use to prevent the abundance of truncated and toxic proteins to protect their balance.In the present study, we reported immunoprecipitation and purification experiments to evaluate the protein interactions which in the next level of experiments will suggest other fundamental and remarkable functions of the NMD-related factors during gene expression. Furthermore, we created new strains by tagging protein factors to assess further whether interactions are direct or indirect. -Growth of fission yeast cells ( Schizosaccharomyces pombe ) -Protein extraction from large scale cultures -Immunoprecipitation and affinity purification of protein samples in different buffer conditions -Western Blotting -Genomic DNA extraction -PCR-based approach of gene targeting Show less



Minotech Biotechnology, IMBB

• ITE,University of Crete,Heraklion,Greece

• Undergraduate Student Thesis

  • Oct 2016 - May 2017

  Faithful synthesis of long cDNA requires thermostable and processive reverse transciptases (RT). Moreover, they have been found that the presence of ribonuclease inhibitor (RI) improve the quality of the products of RT reaction. Aim was the creation of a commercial kit for synthesis of cDNA that will includes above enzymes RT and RI. In the present study, we performed the quality controls of a modified form of a viral RT from mouse which isolated and cloned and also the design, purification and isolation of modified human RI from MINOTECH biotechnology. - In silico design of the gene sequence -Expression and isolation of protein products - Affinity chromatography -Use of PCR and Real-Time Q-PCR technique for the quality controls of the enzymes Show less



Enzyme Biotechnology Laboratory

• University of Crete, Heraklion, Greece

• Research Trainee

  • Jul 2016 - Sep 2016

  Teamwork in a lab of six people Study of peptydoglycan enzymes in Bacillus Cereus and Bacillus anthracis enzymes and their involvement in pathogenesis and antimicrobial resistance. -Protein expression -Chromatography -Transformation of recombinant DNA in bacteria cells Teamwork in a lab of six people Study of peptydoglycan enzymes in Bacillus Cereus and Bacillus anthracis enzymes and their involvement in pathogenesis and antimicrobial resistance. -Protein expression -Chromatography -Transformation of recombinant DNA in bacteria cells