Experience

Eurofins DiscoverX

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist III -Group Lead

  • Jun 2015 - Present

  · Performed Cell Based Assay Development: adherent and suspension cell growth and condition study. · Initiated new study protocols and wrote SOPs · Main provider for plated cells in DiscoverX Profiling PathHunter Services. · Adhere to strict and fast paced turn around Project schedules with 90% one-time pass and on time delivery. · Maintained different mammalian cell lines. Passage, plate, culture and freeze down for bulk stock. · Make weekly schedule and assigned projects to Research Associates. QUALITY CONTROL · Grow and test production cell stocks for functionality (EC50, S:B), sterility and viability. · Performed Luminescence Cell based assays using small molecule ligands against new production cell stocks. Analysis using Prism. • Trained new hired employees in validation for the multi drop cell dispensing machine for daily use and in accordance to strict GMP guidelines. • cGMP certified.



Molecular Devices

• United States
• Biotechnology Research
• 500 - 600 Employee

• Laboratory Manager

  • 2001 - 2007

  RESEARCH ASSOCIATE II 2004 – 2006 RESEARCH ASSOCIATE I 2001 – 2004 • Oversee Cell Culture facility in Union City. • Worked with engineers and scientists for all Cell Culture and Reagent needs. • Coordinated with Marketing for equipment demo and validation. • Performed cell culture validation from customer source or in-house cell stocks to assist customers in running PatchXpress (HTS Ion channel patch clamp). • Team member in the making of the next generation of FLPR (Fluorescence and Luminescence 1534 well reader). • Provided cells for Ironworks chip quality control testing and machine validation. • Oversee inventory and procurement lab disposables, reagents and media. • Performed cell-based assays as needed. • Used Guava Flow cytometry for viability testing. • Safety Committee member. Responsible for the biohazard waste collection and annual health inspection. . Formulate new Cell Culture procedures for new cell lines to adapt to different machine settings/procedures and generate standard protocol for daily use. • Coordinate with scientists and engineers regarding cell usage for demo and experiments. • Perform cell viability experiments in different conditions to find optimum cell condition for PatchXpress. • Safety committee member responsible for biowaste and chemical waste handling.



MedImmune

• United States
• Biotechnology Research
• 700 & Above Employee

• Cell Culture Associate II

  • 1999 - 2001

  • Maintain laboratory protocols, equipment calibrations, and validations in compliance of cGMP • Performed process validation for bringing in Safety Testing for FluMist. • Grew and frozen down different Virus stocks for Safety Testing. • Performed TCID50 ELISA assays and EID50 assays. • Euthanized chicks and harvest their kidneys as part of the Safety Testing. • Point person during Lab Inspection in preparation for company’s product BLA / FDA submission. • Provided plated cells for all QC, Research and Process Development departments in the Santa Clara facility. • Performed daily cell passages, freeze and thaw according to SOP. • Wrote and updated SOP’s. • Performed validation for the multi drop cell dispensing machine for daily use and in accordance to strict GMP guidelines. • Trained new hired employees. • cGMP certified. • Awarded the MVP of the Department in 1999 by peers.