Angela Giorgianni
PHD Student at University of Natural Resources and Life Sciences, Vienna (BOKU)- Claim this Profile
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Italian Native or bilingual proficiency
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English Professional working proficiency
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French Elementary proficiency
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Dutch Elementary proficiency
Topline Score
Bio
Experience
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University of Natural Resources and Life Sciences, Vienna (BOKU)
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Austria
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Higher Education
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700 & Above Employee
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PHD Student
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Nov 2021 - Present
Identification and characterization of Cellobiose Dehydrogenase (CDH) class III. Selection of cdhIII genes and heterologous production using different expression strategies. Development of an efficient purification strategy. Physicochemical, structural, spectral and bioelectrochemical properties charachterization of the obtained CDHs.Task: tools for bioinformatic analysis, molecular biology using different cloning strategies, heterologous expression in yeast in flasks and stirred-tank bioreactors, several chromatographic protein purification techniques, spectrophotometric activity assays. Show less
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Laboratory Manager
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May 2022 - May 2023
Managing the distribution of bench and desk to personnel; welcoming new people and taking care of all required documentation; overseeing equipment responsibilities; planning recurrent maintenance of the laboratory areas.
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The Janssen Pharmaceutical Companies of Johnson & Johnson
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United States
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Pharmaceutical Manufacturing
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700 & Above Employee
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Scientist
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Feb 2019 - Nov 2021
-Tasks: Provide scientific and technical support for fermentation of animal cells to produce Large Molecules for the assessment of a new bioreactor prototype. Authoring and reviewing technical documents in support to the trials and experiments. Writing and improvement of cell culture protocols. Responsible for tracking of project outputs providing any required associated report including status and plan. Data analysis, tabulation (including basic statistical data management). -Techniques: Cell culture techniques (both for adherence and suspension cell lines); bioreactor cultivation of CHO cells and microorganisms (S. cerevisiae, B. subtilis); HPLC analysis, enzymatic assays for quantification of cells metabolites Show less
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Actial Farmaceutica
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Rome, Latium, Italy
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Technical Operation Support Intern
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Sep 2018 - Feb 2019
Support to the Regulatory Affairs department of a company leader on the probiotics field, revision of data coming from partner laboratories, management of data coming from quality analysis, support on the technical dossier writing, revision technical contents on social media among the others. Support to the Regulatory Affairs department of a company leader on the probiotics field, revision of data coming from partner laboratories, management of data coming from quality analysis, support on the technical dossier writing, revision technical contents on social media among the others.
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Institute of Biology Leiden
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Leiden, South Holland, Netherlands
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Intern
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Sep 2017 - Jun 2018
-Tasks: Analysis of UV mutant strains of A. niger for their growth in bioreactors and for their production of Starch Degrading Enzymes. -Techniques: Bioreactor cultivations of fungi, RNA isolation techniques, Northern blot analysis, Western Blot analysis, enzymatic activity assays. -Tasks: Analysis of UV mutant strains of A. niger for their growth in bioreactors and for their production of Starch Degrading Enzymes. -Techniques: Bioreactor cultivations of fungi, RNA isolation techniques, Northern blot analysis, Western Blot analysis, enzymatic activity assays.
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University of Rome Tor Vergata
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Italy
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Research Services
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700 & Above Employee
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Intern
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Mar 2016 - Jun 2016
- Tasks: isolation and amplification of the gene aga1 encoding for an agarase. Construction of a plasmid harbouring aga1 gene and transformation in E. coli BL21(DE3). - Techniques: Techniques of classical and molecular microbiology, PCR. - Tasks: isolation and amplification of the gene aga1 encoding for an agarase. Construction of a plasmid harbouring aga1 gene and transformation in E. coli BL21(DE3). - Techniques: Techniques of classical and molecular microbiology, PCR.
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Education
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University of Rome "Tor Vergata"
Master of Science - MS, Biotechnology -
University of Rome "Tor Vergata"
Bachelor's degree, Biotechnology -
Liceo Gaetano De Sanctis
Diploma, Scientific