Experience

HyTest

• Finland
• Biotechnology Research
• 1 - 100 Employee

• Scientific Researcher

  • Jan 2023 - Present

  In R&D. Downstream process development of purification HBsAg (Virus-like-particles). In R&D. Downstream process development of purification HBsAg (Virus-like-particles).



Generium

• Russian Federation
• Biotechnology Research
• 1 - 100 Employee

• DSP Junior Scientist

  • Jun 2022 - Jan 2023

  R&D, department of cell biology, laboratory of gene therapy drugs. Responsibilities: development of purification methods and chromatography purification of AAV. Work with AKTA chromatography and TFF systems (Pure, Pilot, Flux), affinity, multimodal, ionexchange chromatography; analytical methods: SDS-PAGE, western blotting, SF, DLS (stUNer®), and other. Achievements: - Affinity chromatography using X sorbent gives yield of AAV particles over 95% (max sample volume - 200L) - Successful application of anionexchange chromatography to separate empty/full particles of AAV - Using Z multimodal resin decrease contamination of hcDNA in 30 times. Show less



Generium

• Russian Federation
• Biotechnology Research
• 1 - 100 Employee

• USP Laboratory assistant/technician

  • Jun 2021 - Oct 2021

  In the same laboratory. Responsibilities: work with HEK293 cell line in clean box, cells counting, cultivating in bioreactor and flasks, transfection and lysis, dead-end filtration and other. In the same laboratory. Responsibilities: work with HEK293 cell line in clean box, cells counting, cultivating in bioreactor and flasks, transfection and lysis, dead-end filtration and other.



Central Research Institute for Epidemiology

• Laboratory Assistant

  • Sep 2020 - Jun 2021

  In the laboratory of genomic editing. Work in the field of CRISPR/Cas gene therapy. Responsibilities: gene engineering, enzyme synthesis in prokaryotic cells, IMAC chromatography purification, PCR, agarose gels, SDS-PAGE and other. Achievement: 2 ferments were synthesized in E.Coli platform and purified using His-tag IMAC chromatography. In the laboratory of genomic editing. Work in the field of CRISPR/Cas gene therapy. Responsibilities: gene engineering, enzyme synthesis in prokaryotic cells, IMAC chromatography purification, PCR, agarose gels, SDS-PAGE and other. Achievement: 2 ferments were synthesized in E.Coli platform and purified using His-tag IMAC chromatography.