Experience

The EVERY Company

• United States
• Food and Beverage Manufacturing
• 1 - 100 Employee

• Analytical Operations Lead

  • Jun 2023 - Present

• Senior Research Associate

  • Mar 2022 - Jun 2023

• Research Associate II - Automation and Analytics

  • Jan 2021 - Apr 2022



Intrexon Corporation

• United States
• Biotechnology
• 1 - 100 Employee

• Research Associate II

  • Aug 2018 - Feb 2020

  • Refinement and optimization of enzyme-coupled assay for kinetic analysis. • Assisted overseeing and generating a plasmid library for tagged-protein purification POC project in E.coli • Developed plate-based biomass build process to generate samples for medium-scale purification • Transitioned purification scheme for protein of interest from a manual process to an automated process. • Continued tasks from internship (HTS screening, protein purification, kinetics studies, and operations optimization) in methanotroph. • Maintained up to date documentation while presenting data through written reports and cross-team presentations, supported through LIMS. Show less



Boston University

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Sep 2017 - Jul 2018

  • Performed an RNAi screen of kinase activity during ovary development using the GAL4/UAS system • Antibody stained and examined tissue sections of ovaries through immunofluorescent microscopy. • Followed up and confirmed hits from screen through qPCR to ensure knockdown of targeted kinase. • Created plasmid construct using Gateway cloning and plasmid purification to use for injection for transgenic tool development. • Performed an RNAi screen of kinase activity during ovary development using the GAL4/UAS system • Antibody stained and examined tissue sections of ovaries through immunofluorescent microscopy. • Followed up and confirmed hits from screen through qPCR to ensure knockdown of targeted kinase. • Created plasmid construct using Gateway cloning and plasmid purification to use for injection for transgenic tool development.



Intrexon Corporation

• United States
• Biotechnology
• 1 - 100 Employee

• Intern (Enzymology and Assay Development)

  • Jun 2017 - Aug 2017

  • Developed untagged protein purification process • Optimized cell lysis techniques and different column chromatography techniques including size exclusion, anion exchange, and hydrophobic interaction • Piloted preliminary characterization of isolated mutant and wildtype protein using coupled kinetic assays to measure kcat and km • Screened for mutants using high-throughput automation methods and gas chromatography/mass spectroscopy • Developed untagged protein purification process • Optimized cell lysis techniques and different column chromatography techniques including size exclusion, anion exchange, and hydrophobic interaction • Piloted preliminary characterization of isolated mutant and wildtype protein using coupled kinetic assays to measure kcat and km • Screened for mutants using high-throughput automation methods and gas chromatography/mass spectroscopy



Boston University School of Medicine

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Research Assistant

  • Aug 2015 - May 2017

  • Performed experiments to identify cancer targeting antibodies within human plasma samples using various techniques like ELISA, gel electrophoresis, SDS-PAGE, Western blot, flow cytometry and hybridoma cell culture • Isolated monoclonal antibodies from cell lysate using column affinity chromatography. • Designed experiments with Dr. Sharon, interpreted results, and determined next directions for individual projects as well as overall lab research • Maintained day-to-day functioning of the lab including restocking and cleaning lab benches and filling liquid nitrogen dewars Show less