Experience

Conagen

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist I

  • May 2022 - Present

  • Identify candidate protein targets to onboard • Expression of proteins in secretory, intracellular (soluble) and inclusion body fraction in E. coli • Design project outline, construct plasmids and test expression under several different promoters, RBS, secretion signal sequences in E. coli and Bacillus subtilis for optimization of protein expression • Express polymer proteins as inclusion bodies in engineered E. coli and optimize the method for downstream purification the protein • Collaborate extensively across groups to design, develop and troubleshoot protein expression related experiments Show less



University of Minnesota College of Biological Sciences

• United States
• Higher Education
• 100 - 200 Employee

• Postdoctoral Research Associate

  • Feb 2019 - Feb 2022

  Developed Bacillus subtilis 168 as a platform strain to secrete normally non-secreting cytoplasmic protein for fabrication of engineered living biocomposite material • Engineered Bacillus subtilis 168 to (i) prevent cell lysis thus release of endospore, (ii) localization of flagella on polar region • Engineered flagellin protein for display of functional peptide tag for attachment with self-assembling protein on flagella of Bacillus subtilis • Optimized secretion and analysis method for self-assembling cytoplasmic protein (EutM) • Developed a well annotated vector system and optimized transformation method for heterologous gene expression in Deinococcus radiodurans • Collaborate with multidisciplinary research team for planning the course of experiment to reach the research target Show less



University of South Florida

• United States
• Higher Education
• 700 & Above Employee

• Postdoctoral Researcher

  • Jul 2018 - Feb 2019

  • Tested antibacterial peptides and nano-particles against culturable proxy plant pathogen for citrus greening (Liberibacter crescens) • Isolation of bacterial strains from wastewater samples to understand the microbial community composition before and after treatment • Compared microbial communities from reclaimed wastewater before and after treatment using high throughput 16S rRNA sequencing and analysis • Prepared grant proposals, SOPs and research papers • Trained junior researchers and freshmen the use of aseptic techniques and strain characterization workflows • Presented periodic updates, analyzed data and prepared manuscript as needed Show less