Amber Nadareski
Research Technician at Jacobs School of Medicine and Biomedical Sciences at the University at Buffalo- Claim this Profile
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Bio
Credentials
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Making a cell therapy: Principles and Practices of Manufacturing
HarvardXApr, 2021- Oct, 2024 -
Molecular Biology
HarvardXDec, 2020- Oct, 2024 -
Basic Analytical Chemistry
HarvardXSep, 2020- Oct, 2024 -
Drug Discovery and Medicinal Chemistry
HarvardXSep, 2020- Oct, 2024 -
Principles of Biochemistry
HarvardXSep, 2020- Oct, 2024
Experience
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Jacobs School of Medicine and Biomedical Sciences at the University at Buffalo
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United States
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Higher Education
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200 - 300 Employee
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Research Technician
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Jan 2023 - Present
Performed experiments on mice involving injection of vaccines or nanoparticle treatment formulations, including dissection and administration of anesthetics for harvesting of tissues/fluids for analysis. Analysis of compounds and their molecular activities using immunological techniques: flow cytometry, ELISA, PCR, florescence imaging, electrophoresis Maintenance of tissue cultures for experimentation using cell isolations and cell cultures of established cell lines. Produced plaque reduction assays on cell lines to determine effectiveness of in vivo and in vitro treatment by measuring viral titers in blood and lung homogenates from mice. Formulated liposome targeted nanoparticles loaded with PLGA/PVA/chitosan encapsulating siRNA to inhibit the viral NS1 gene as treatment for influenza Show less
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Bright Horizons
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Education Administration Programs
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1 - 100 Employee
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Early Childhood Educator
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2016 - 2018
Executed and planned structured classroom activities while overseeing student behaviors while assessing developmental needs of individuals within principles of early childhood development including documentation. Executed and planned structured classroom activities while overseeing student behaviors while assessing developmental needs of individuals within principles of early childhood development including documentation.
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Fred Hutch
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United States
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Hospitals and Health Care
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700 & Above Employee
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Immune Monitoring Specialist
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2015 - 2015
A resource lab providing investigators with services and expertise for monitoring cancer patient immunities undergoing CAR-T based infusions within clinical trials. Measured cytokine and bio modulators in patient and cell culture samples using Luminex and ELISA assays, TCR spectratyping, and using T-PCR to measure T-clone expansion and adoptive transfer in therapy trials of cancer patients. A resource lab providing investigators with services and expertise for monitoring cancer patient immunities undergoing CAR-T based infusions within clinical trials. Measured cytokine and bio modulators in patient and cell culture samples using Luminex and ELISA assays, TCR spectratyping, and using T-PCR to measure T-clone expansion and adoptive transfer in therapy trials of cancer patients.
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Quality Control Analyst
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2013 - 2014
Developed, performed, and optimized assay qualification/validation studies and analytical SOPs for product testing while establishing test methods and equipment for GMP compliance in research and development. Application of analytical test procedures through process sciences, manufacturing, and quality control for clinical product testing raw materials and in-process using antibodies addressing medical conditions. Tested robustness, concentration, purity and optimal conditions of samples for clinical trials using CE-SDS. Show less
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Wadsworth Center
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United States
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Hospitals and Health Care
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1 - 100 Employee
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Graduate and Research Assistant
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2012 - 2013
Studied functional genomics of cellular growth of ERBB2+/neu expressed breast cancer cells in fat synthesis detecting co-expressed genes using shRNA libraries qRT-PCR for cDNA, SDS PAGE, primer design sequencing, and western blotting. Studied colon cancer and inflammation associated with low levels of vitamin D3 through cell lines MCF-7 and CT26 using crystal violet imaging, microscopy, cell culture proliferation assays and western blots for vitamin D3 receptors. Studied functional genomics of cellular growth of ERBB2+/neu expressed breast cancer cells in fat synthesis detecting co-expressed genes using shRNA libraries qRT-PCR for cDNA, SDS PAGE, primer design sequencing, and western blotting. Studied colon cancer and inflammation associated with low levels of vitamin D3 through cell lines MCF-7 and CT26 using crystal violet imaging, microscopy, cell culture proliferation assays and western blots for vitamin D3 receptors.
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Biology Tutor
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2010 - 2012
Handled students on a one-to-one basis and in small group settings to teach the foundations of general biology and chemistry along with basic theories and mathematics.Acted as a friendly mentor to all students by teaching on a one-to-one basis and in large group settings about experimental methods in interactive ways to help students gain a better understanding of chemistry and biology.
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Research Assistant - African Sleeping Sickness Laboratory
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2010 - 2012
Assessed the function of the protein “Tblpn” involved in lipid biosynthesis from the African sleeping sickness parasite, Trypanosoma brucei, in terms of cellular growth, lipid synthesis, and the expression of lipid synthesizing genes.Worked in a small laboratory setting with graduate students to collectively gather data on multiple experiments on Tblpn, such as protein methylation and phosphorylation in vivo using enzymatic assays.
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Chemistry Laboratory Assistant
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2009 - 2012
Engaged students in general chemistry and organic chemistry labs in laboratory techniques, fundamental molecular knowledge, and personal safety on bench work. Practiced OSHA safety regulations to ensure safe environment by enforcing safety protocol, upkeep ofchemical stock and lab equipment, and laboratory logbooks.Practiced chemical safety regulations to create a safe working environment for self and all student peers by teaching and enforcing safety protocol, as well as upkeep of the chemical stock room holding all chemicals and lab equipment used, and ensured proper use of materials by students. Show less
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Education
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2008 - 2012
State University of New York College at Brockport
Bachelor of Science (B.S.), Biological Sciences and Chemistry
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