Experience

California Cultured Inc.

• United States
• Biotechnology
• 1 - 100 Employee

• Associate Scientist

  • Aug 2022 - Present



Green Venus, LLC (previously Intrexon AgBio)

• Davis, California

• Scientist

  • Apr 2016 - Oct 2022

  Support molecular and tissue culture team leads to enable genome editing in vegetable and tree crops Plant protoplast isolation, transfection, culture, and regeneration: Maintain suspension cultures or clonally propagated vegetative lines in vitro. Optimize methods to isolate viable protoplasts from a variety of explants, enhance transfection efficiency of plasmids into protoplasts, and develop assays to validate transient expression and function of transgenes. Culture of transfected protoplasts to regenerable calli Genome editing: Assessment of native gene expression by qRT-PCR. Vector design and construction. Selection of guideRNAs and validation of activity through NextGen sequencing of transfected cells. Characterization of regenerated events for transgene copy number by qPCR, Sanger sequencing of genome edited targets, and identification of transgene integration sites by Genome Walker. Written reports and oral presentations to communicate experimental results and recommendations of next steps. Supervision, training, and mentoring of junior employees in molecular biology and tissue culture techniques Management of common-stock supplies and general lab maintenance Show less



Viridos

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Associate

  • Jul 2011 - Mar 2016

  Algal metabolic engineering: cloning genes of interest, transformation of DNA into multiple algal strains, and follow-up gene validation in transformed lines by genotyping, protein expression analysis, qRT-PCR, biochemical assays, and productivity assays Report writing Participation in training of new employees and management of contract workers Management of common-stock supplies Algal metabolic engineering: cloning genes of interest, transformation of DNA into multiple algal strains, and follow-up gene validation in transformed lines by genotyping, protein expression analysis, qRT-PCR, biochemical assays, and productivity assays Report writing Participation in training of new employees and management of contract workers Management of common-stock supplies



University of Nevada, Reno

• United States
• Higher Education
• 700 & Above Employee

• Graduate Researcher

  • Jan 2010 - May 2011

  Expression studies of rubber biosynthetic genes in transgenic Nicotinia tobaccum to correlate gene expression with production of novel long-chain polyiosoprene product Teaching undergraduate students general laboratory techniques Expression studies of rubber biosynthetic genes in transgenic Nicotinia tobaccum to correlate gene expression with production of novel long-chain polyiosoprene product Teaching undergraduate students general laboratory techniques



Nevada Cancer Institute

• Las Vegas, Nevada

• Student Intern

  • Jun 2010 - Aug 2010

  Molecular cloning to express uridine phosphorylase as a fusion protein in mammalian cell culture to identify binding partners Molecular cloning to express uridine phosphorylase as a fusion protein in mammalian cell culture to identify binding partners



Charles River Laboratories

• United States
• Biotechnology Research
• 700 & Above Employee

• Animal Care Technician

  • Jun 2008 - Oct 2009

  Husbandry tasks including feeding, environmental enrichment, and medical treatment of canine and primate research animals Compliance with GLP and regulations regarding the use of animal models in research Husbandry tasks including feeding, environmental enrichment, and medical treatment of canine and primate research animals Compliance with GLP and regulations regarding the use of animal models in research