Aliki Valdes
PHD Candidate at Northwestern University- Claim this Profile
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Bio
Experience
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Northwestern University
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United States
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Higher Education
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700 & Above Employee
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PHD Candidate
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Sep 2021 - Present
Driskill Graduate Program in Life Sciences Advisor: Alan Hauser, MD/PhD Project: High throughput infection models to identify accessory genomic elements (AGEs) driving Pseudomonas aeruginosa cytotoxicity Driskill Graduate Program in Life Sciences Advisor: Alan Hauser, MD/PhD Project: High throughput infection models to identify accessory genomic elements (AGEs) driving Pseudomonas aeruginosa cytotoxicity
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University of Chicago
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United States
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Higher Education
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700 & Above Employee
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Research Technician
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Aug 2020 - Jun 2021
NIH Postbaccalaureate Research Education Program (PREP) Advisor: Glenn Randall, PhD Project: Control of Hepatitis C Virus Infection by Cellular RNA Degradation Machinery NIH Postbaccalaureate Research Education Program (PREP) Advisor: Glenn Randall, PhD Project: Control of Hepatitis C Virus Infection by Cellular RNA Degradation Machinery
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Northwest Laboratory
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United States
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Medical Practices
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1 - 100 Employee
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Molecular/PCR Technician
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Mar 2020 - Jul 2020
- Preform RNA extractions for SARS-CoV-2 testing - Operate INTEGRA electronic pipettes and pipetting robots - Prepares reagents and other consumables for extraction procedures - Performs routine cleaning and maintenance of equipment, supplies and assigned work area. - Preform RT-qPCR based SARS-CoV-2 testing - Preform RNA extractions for SARS-CoV-2 testing - Operate INTEGRA electronic pipettes and pipetting robots - Prepares reagents and other consumables for extraction procedures - Performs routine cleaning and maintenance of equipment, supplies and assigned work area. - Preform RT-qPCR based SARS-CoV-2 testing
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Western Washington University
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United States
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Higher Education
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700 & Above Employee
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Microscopy Teaching Assistant
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Jan 2020 - Mar 2020
- Train 400-level students to image Tetrahymena on a DeltaVision Deconvolution microscope - Troubleshoot upright and inverted microscopes - Mentor students through coursework and projects using ImageJ and vector-based graphics. - Use DeltaVision Deconvolution microscope to render 3-D fluorescent videos of deblurred specimens- Immunofluorescence staining to visualize cilia and centrosomes
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Researcher
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Sep 2019 - Mar 2020
- Mammalian cell culture with 3T3 cells and hTERT-RPE cells- Fluorescence microscopy with a Nikon TI2 microscope running on MicroManager- Preform molecular cloning and transfections- Investigating protein trafficking between cilia and the Golgi apparatus
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Organic Chemistry Teaching Assistant
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Sep 2018 - Dec 2019
- Respond to chemical spills or safety hazards- Prepare common lab solutions and reagents with clear labels and well-documented descriptions- Operate IR machine, rotary evaporator, and other lab equipment - Evaluate 300-level student assignments and troubleshoot experiments
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Northwestern University
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United States
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Higher Education
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700 & Above Employee
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Researcher (NSF REU)
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Jun 2019 - Sep 2019
- Recombination-mediated genetic engineering (recombineering) on S.enterica and E.coli - Streak plating and serial dilutions for bacterial enumeration - Protein secretion assays & western blotting to visualize protein expression and protein secretion titers - Valdes A., Burdette L.A., Wong H.T., Ikonmova S.P., Tullman-Ercek D. (August 2019). The effects of pathogenic gene knock out on protein secretion titer in Salmonella enterica serovar Typhimurium. Presented at SYNBREU Symposium, Evanston, IL. Show less
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Education
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Western Washington University
Cellular & Molecular Biology, Minor in Chemistry