Experience

VIQI group

• France
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Healthcare Industry Project Manager

  • May 2022 - Present

  - Guarantee technical projects between sites or international and on different products - Guarantee technical and scientific support and expertise for critical business projects - Support major continuous improvement projects requiring in-depth product/process expertise - Identify tasks, plan and manage project-related activities by mobilizing the teams concerned and ensuring compliance with deadlines and the level of resources (project management) - Prepare and lead project meetings by setting up the necessary supports and tools - Build, write, review or approve protocols and reports - Management of minor/major laboratory investments - Laboratory EHS risk management - Analyze, anticipate and manage technical difficulties and risks related to projects and services Show less



EUROIMMUN France

• France
• Biotechnology Research
• 1 - 100 Employee

• Technical Sales Engineer

  • Sep 2021 - Oct 2021

  - Present and promote products and services - Respect and enforce sales conditions - Monitor sales - Analyze customer needs - Present and promote products and services - Respect and enforce sales conditions - Monitor sales - Analyze customer needs



Institut Hospitalo-Universitaire de Marseille

• Researcher (PhD) in Biology and Health, specializing in infectious diseases (in thesis)

  • Oct 2017 - Mar 2021

  Vaginal microbioime and dysbiosis, Humain papillomavirus infection and Cervical intraepithelial neoplasia : Cervical cancer is a pathology with infectious origin, which the main cause is the persistence of human papillomaviruses infections, mainly HPV16/18. Several studies suggest an association of bacterial vaginosis (BV) with a higher prevalence and persistence of vaginal HPV infections as well as a correlation with the development of cervical lesions. BV is a vaginal infection characterized by a massive decline of Lactobacilli replaced by pathogenic bacteria. The aim of this thesis is to study tstudy of the vaginal microbiota and virome in physiological and pathological contexts (BV and HPV infections).The first two chapters have contributed to the increase of knowledge on the vaginal microbiota via the description of 4 new bacterial species isolated from women with BV and the metagenomic analysis of the composition and the dynamics of the vaginal virome in healthy subjects or those suffering from BV followed before, during and after antibiotic treatment. Preliminary and promising results of the in vitro effect of L. jensenii and astaxanthin, on the E6 and E7 oncogene expression levels of as well as in the inflammatory response and the viability of tumor cells with integrated HPV16 or HPV18, are also presented. The third chapter describes the induction, sequencing and characterization of 3 new vaginal phages. Finally, the fourth chapter details the development and clinical validation of powerful tools for the detection and quantification of the E6 and E7 oncogenes of HPV16 and HPV18 which can be used as additional supports for traditional HPV assays or as a second intentions follow-up of patients at risk of developing lesions. Show less

• Research assistant (6-month internship)

  • Jan 2017 - Jun 2017

  SUBJECT : Composition, dynamics and functional analyzes of the vaginal viroma in the context of bacterial vaginosis SUPERVISION: Dr. Christelle Desnues SUMMARY: Bacterial vaginosis is caused by a vaginal flora imbalance (dysbiosis) whose etiology is not known. This pathology is found in 10% to 50% of fertile, premenopausal and pregnant women and several studies have suggested an association between vaginosis, human papillomavirus (HPV) infections and cervical cancer. Recent high-throughput sequencing approaches have allowed to bypass cultivation and knowledge of genomes and to study and characterize all viral genomes in a sample. So far, the exhaustive inventory of the vaginal virome in a context of vaginosis has not been done. In this work, we studied the virome of controls and patients with vaginosis before, during and after antibiotic treatment. We have shown a high prevalence and abundance of oncogenic high-risk HPVs and observed a dynamic of these viruses over time in patients with vaginosis. Except HPVs, only few other viral sequences have been detected and we hypothesized that many sequences have been mistakenly annotated as bacteria. Using mitomycin C, we induced prophages of L. crispatus in order to increment the phage database, improve the metagenome annotation and thus, gain a better understanding of vaginosis etiology. We also discovered a strain of L. jensenii with antiviral activity and potentially oncoprotective effect. We also have shown that G. vaginalis, a common bacterium in dysbiosis as well as the antibiotics used in the treatment of vaginosis (clindamycin and metronidazole) are factors with a proviral activity. Finally, we proposed that antibiotic treatment and vaginal dysbiosis induce the production of HPVs whose role in the development of cervical cancer remains to be studied. METHODS: standard and quantitative PCR, culture of cell line and bacterial strains, preparation of metagenomes, bioinformatic analyzes Show less

• Research assistant (3-month internship)

  • Apr 2016 - May 2016

  SUBJECT: Study of the dynamics of viral populations in the context of antibiotic treatment of a patient with chronic vaginosis SUPERVISION: Dr. Christelle Desnues SUMMARY: Vaginosis is a vaginal infection caused by a vaginal bacterial flora imbalance whose etiology is not known. This pathology is found in 10% to 50% of fertile, premenopausal and pregnant women and several studies have suggested an association between vaginosis and human papillomavirus (HPV) infections. The recent development of high-throughput sequencing technology has allowed studying and characterizing all viruses in a sample (the virome) without the need of culture or knowledge of the viral genome. So far, an exhaustive study of the vaginal virome in case of vaginosis has not been done. In the present work, we have studied the virome of a woman with a 1 year-chronic vaginosis before/during and after antibiotics. We have shown that the vaginal flora is dominated by HPVs. We have observed and experimentally validated a dynamic of HPV populations over time. Potentially oncogenic HPVs (HPV18 and 33) appear during antibiotherapy whereas others (HPV91) increase in abundance. We hypothesized that antibiotherapy induces an HPV production whose consequences, particularly in case of oncongenic HPVs, are unknown. METHODS USED: PCR, qPCR, bioinformatics tools (blast, diamond ,. ..) Show less