Alexandros Galaras

Researcher Phd Candidate at BSRC Alexander Fleming
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Contact Information
us****@****om
(386) 825-5501
Location
Nea Smyrni, Attiki, Greece, GR
Languages
  • English Full professional proficiency
  • Greek Native or bilingual proficiency
  • Italian Native or bilingual proficiency

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Credentials

  • The Volos Summer School of Human Genetics
    Wellcome Sanger Institute & University of Thessaly
    May, 2018
    - Nov, 2024

Experience

  • BSRC Alexander Fleming
    • Greece
    • Research Services
    • 1 - 100 Employee
    • Researcher Phd Candidate
      • Jan 2019 - Present

      Title: "Identification and functional characterization of lncRNAs implicated in colorectal homeostasis and carcinogenesis" Mainly focused on studying lncRNAs and translatable smORFs residing in the non-coding genome through functional experiments and -omic approaches. My work has also expanded through collaborations to characterize the contribution of transcription factors in transcriptomic and chromatin accessibility profiles in other cell populations and diseases. ● DNA/RNA/Protein extraction, Bacterial culture, Molecular cloning, PCR, Western Blotting, immunofluorescence ● Mammalian cell culture handling, FACS, Transfection, Lentiviral transduction ● ChIP, RIP ● CRISPR-Cas9 ● Analysis of RNA-seq | ChIP-seq | ATAC-seq | RIP-seq ● Analysis of clinical data (TCGA, Organoid Biobank) ● Proteomic analysis (MaxQuant, Perseus) Show less

  • BSRC Alexander Fleming
    • Greece
    • Research Services
    • 1 - 100 Employee
    • Graduate Student Researcher
      • Sep 2017 - Oct 2018

      Pantelis Hatzis Lab Thesis title: "Investigating the role of WiNTRLINC3 in intestinal homeostasis and carcinogenesis" My main goal was to characterize the mechanism of function of a Wnt-regulated lncRNA and its interacting proteins in colorectal cancer cells. ● DNA/RNA/Protein extraction ● Bacterial culture, Molecular cloning, PCR, Western Blotting, ● Transfection, Lentiviral transduction ● RNA-seq/Chip-seq analysis Pantelis Hatzis Lab Thesis title: "Investigating the role of WiNTRLINC3 in intestinal homeostasis and carcinogenesis" My main goal was to characterize the mechanism of function of a Wnt-regulated lncRNA and its interacting proteins in colorectal cancer cells. ● DNA/RNA/Protein extraction ● Bacterial culture, Molecular cloning, PCR, Western Blotting, ● Transfection, Lentiviral transduction ● RNA-seq/Chip-seq analysis

  • Biomedical Research Foundation of the Academy of Athens
    • Greece
    • Research Services
    • 100 - 200 Employee
    • Graduate Student Intern
      • May 2017 - Jul 2017

      Title: "Bioinformatics and Proteomics analysis of Bladder cancer tumors" I was responsible for the proteomic analysis of multiple clinical samples to discover novel biomarkers in Bladder cancer. ● Proteomics analysis (Protein Discovery, Differential expression analysis) ● Clustering methods Title: "Bioinformatics and Proteomics analysis of Bladder cancer tumors" I was responsible for the proteomic analysis of multiple clinical samples to discover novel biomarkers in Bladder cancer. ● Proteomics analysis (Protein Discovery, Differential expression analysis) ● Clustering methods

  • BSRC Alexander Fleming
    • Greece
    • Research Services
    • 1 - 100 Employee
    • Graduate Intern
      • Feb 2017 - Apr 2017

      Title: "Bioinformatic analysis of the De-regulome, upon WiNTRLINC3 perturbation in colorectal cancer cells" Even though I pursued my studies in Molecular biology, my main field of interest I wanted to expand my skills and understanding to the world of bioinformatics. I analyzed RNA-seq experiments from our lab to characterize the transcriptomic profile of colorectal cancer cells that overexpressed the lncRNA of interest. ● Unix command-line | R programming ● RNA-seq analysis ● Chip-seq analysis Show less

  • University of Patras
    • Greece
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Research Fellow
      • May 2015 - Sep 2016

      Laboratory of Molecular Genetics, Dr Moschonas Nikolaos Thesis title: "Contribution to the functional analysis of DGCR14 utilizing cellular models with altered expression" ● DNA/RNA extraction, PCR, Bacterial culture ● Eucaryotic cell line handling-Stable cell line generation ● Immunofluorescence Laboratory of Molecular Genetics, Dr Moschonas Nikolaos Thesis title: "Contribution to the functional analysis of DGCR14 utilizing cellular models with altered expression" ● DNA/RNA extraction, PCR, Bacterial culture ● Eucaryotic cell line handling-Stable cell line generation ● Immunofluorescence

Education

  • National & Kapodistrian University of Athens
    Master's degree, Molecular Biomedicine-Disease Mechanisms, Molecular and Cellular therapies, and Bioinnovation
    2016 - 2018
  • University of Patras
    Bachelor's degree, Biology, General
    2012 - 2016

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