Experience

Henry M Jackson Foundation

• United States
• Non-profit Organizations
• 1 - 100 Employee

• Research Laboratory Manager

  • Jul 2017 - May 2018

  Bethesda MD



BioMarker Strategies LLC

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Scientist

  • Mar 2015 - Feb 2017

  Rockville, Maryland



2020 gene

• United States
• Biotechnology
• 1 - 100 Employee

• Project Manager

  • Sep 2010 - Aug 2014

  Development of companion diagnostic for Kidney.: Identify biomarkers on mTor pathway that will be predictive of response to Rapamycin and the “rapalogs’ for patients with Kidney cancer. Develop multiplex IHC technology: research and adapt techniques of IHC to detect up to 10 markers from one tissue section.

• scientist

  • Nov 2008 - Sep 2010

  o Design experiences, write and execute protocols, analyze results for multiplex immunohistochemistry phase II development. o Optimize and trouble-shoot existing protocols (increase layer number, validate Antibodies, improve signal to noise ratio). o Write SOPs. o Demonstrated proof of concept of the new technology in development (specificity of hybridization of fluorescent Oligonucleotides (ODN) with complementary ODN immobilized on polycarbonate membrane. o Prepared conjugated… Show more o Design experiences, write and execute protocols, analyze results for multiplex immunohistochemistry phase II development. o Optimize and trouble-shoot existing protocols (increase layer number, validate Antibodies, improve signal to noise ratio). o Write SOPs. o Demonstrated proof of concept of the new technology in development (specificity of hybridization of fluorescent Oligonucleotides (ODN) with complementary ODN immobilized on polycarbonate membrane. o Prepared conjugated Antibodies (Biotinylated-; Streptavidin-, ODN-Antibodies). o Present results in the weekly lab meeting and summarized them every three months for the quarterly report to NIST.



Bioset

• 1 - 100 Employee

• Research associate

  • Aug 2006 - Apr 2008

  Drug development for medical device o Developed ELISA for synthetic peptide detection in Biological fluids (serum, plasma and urine). o Cultured cell lines (murine myoblast cell C2C12) and pluripotent stem cells (C3HT1/2). o Developed protocol for in vitro study of chondrogenic differentiation model. o Investigated biological activities and physical properties of mimetic peptides of synthesized by Bioset (primarily mimetic of TGF-beta superfamily peptides). o Performed… Show more Drug development for medical device o Developed ELISA for synthetic peptide detection in Biological fluids (serum, plasma and urine). o Cultured cell lines (murine myoblast cell C2C12) and pluripotent stem cells (C3HT1/2). o Developed protocol for in vitro study of chondrogenic differentiation model. o Investigated biological activities and physical properties of mimetic peptides of synthesized by Bioset (primarily mimetic of TGF-beta superfamily peptides). o Performed validation procedures. o Wrote SOPs. o Tested efficiency of peptides coating on different meshes. Show less



Children's Hospital Boston

• United States
• Truck Transportation

• Research fellow

  • Jan 2003 - Dec 2005

  Mechanical stress and cell adhesion o Cultured and transfected endothelial cells (HUVECs, MVEC etc…) using Amexa technology. o Characterized the response of Intergrin following mechanical stimulations. o Contributed to proposal renewal, wrote scientific papers, initiated scientific collaborations. o Presented in international meeting. o Supervised summer intern (Harvard REU program)



Justus Liebig Universität

• Germany
• Higher Education

• Head molecular biology laboratory

  • Apr 2002 - Dec 2002

  o Supervised the molecular biology laboratory. o Trained students and technician in molecular biology techniques. o Characterized Pex19 expression in mouse tissues.



NIAID Laboratory of Allergic Diseases, signal transduction section

• Post-doctoral fellow

  • Feb 1998 - Feb 2002

  Regulation of GPCR signaling pathway in response to activation of EGFr. Using HEK 293T and CHO cells, cultured in Petri Dishes: o Developed and optimized enzymatic and binding assays. o Expressed and purified recombinant proteins from transformed bacterial cultures. o Transfected eukaryotic cells. o Presented scientific discoveries in international meetings. o Supervised summer interns (assigned the projects and trained them in the techniques used).