Principal Investigator (adiunkt)

• Sep 2021 - Present

PostDoc Fellow

• Jun 2019 - Jun 2020

- Writing: 1) independent development of a research proposal, 2) writing a manuscript - Independent management of an interdisciplinary research project (plasma membrane biology, cancer research, 3D cell biology, cell imaging) - Setting up a novel 3D cellular model in the research group - Establishment of immunofluorescence stainings of epithelial cells cultured in 3D cell culture system - Initiation of an international collaboration - Managing reagent orders - Analysis of mass spectrometry data on protein-protein interactions: STRING, Panther, DAVID Bioinformatics - Techniques e.g. Zeiss 710 Confocal Microscope, starvation assay, endocytosis assay; planned for the future: spinning disk confocal microscopy, epithelial–mesenchymal transition in vitro, siRNA knockdown technique, cell transfection, CRISPR/Cas9 - Workshop attendance: IMARIS (microscopy 2D, 3D, and 4D image analysis software), Nov. 21, 19, Umeå University - Attendance to scientific meetings e.g. 10 Years of the Laboratory for Molecular Infection Medicine Sweden (MIMS) - Symposium 18 June 2019, Umeå University, Sweden; KBC Days 2019, 5-6 November 2019, Chemical Biological Centre, Umeå University - Training: plasma membrane biology & cell imaging Show less

Postdoctoral Research Scientist

• May 2017 - Apr 2018

- Writing: developed an independent research proposal and applied for two postdoctoral fellowships: Long-term Human Frontiers in Science (HFSP) postdoctoral fellowship and Department of Defense (DoD) Prostate Cancer Research Program Early Investigator Research Award - Independent research project management: project design, project initiation, performance of experiments, data analysis, interpretation/discussion of results - Supervising/mentoring experience: helped a graduate student with immunofluoresecent staining of mouse bladder tissue and epithelial organoids for stromal cell markers - Laboratory and computational techniques: dissection of the prostate, isolation of urogenital sinus from E15.5 and E16.5 mouse embryos, urogenital sinus explant culture, cryosectioning, immunofluorescent staining of PFA-fixed frozen tissue slices and PFA-fixed cells seeded into chamber slides, confocal microscopy (Leica TCS SP5), basics of mouse husbandry and breeding, setting up timed pregnant mice, isolation and culturing of primary urogenital mesenchymal cells, establishment of isolation and culture of myofibroblasts derived from human prostate cancer tissue, single cell analysis of stromal subtypes in the prostate (software: 10x genomics) - Generated positive controls for immunofluoresecent stainings and established immunofluoresecent stainings for stromal cell markers such as aSMA, vimentin and desmin - Established 13 stromal cell lines derived from human prostatectomy samples - Discovered novel stromal cell subtypes in the mouse prostate - Attendance to conferences and other scientific meetings e.g. The New York Stem Cell Foundation Conference, Cell Press LabLinks Meeting: Metastasis: Translating Biology into Therapy; An AACR Special Conference on Prostate Cancer: Advances in Basic, Translational, and Clinical Research, Cancer Genomics and Mathematical Data Analysis Symposium, and workshops e.g. NIH Workshop - Training: experimental urology Show less

Postdoctoral Research Scientist

• Oct 2016 - Apr 2017

1. Conducting research.2. Scientific writing.3. Training and supervising junior scientists.

PhD student

• Aug 2011 - Sep 2016

- Establishment of epithelial cell and fibroblast isolation from mouse tissue & human biopsy from the gastrointestinal tract- Culturing mouse intestinal epithelial cells and human Barrett´s Esophagus epithelium in the form of organoids (monocultures and co-cultures)- Establishment of immunohistochemistry stainings on cultured intestinal crypts- Investigating molecular mechanisms regulating epithelial-stromal interactions in the gastrointestinal tract- Training: cellular and molecular gastroenterologyPhD dissertation: Organoids as a model to study the cellular microenvironment and as a potential tool for personalized cancer medicine In addition to the research, I have trained and supervised 7 undergraduate students during their internships. Show less

Researcher

• Sep 2009 - Jan 2010

- Techniques: isolating myeloid-derived suppressor cells from mouse spleen, real-time PCR - Training: tumor immunology - Techniques: isolating myeloid-derived suppressor cells from mouse spleen, real-time PCR - Training: tumor immunology

Master Thesis Student

• Oct 2007 - Jul 2009

Molecular chaperones Master thesis: Wpływ poziomu ekspresji białek opiekuńczych na fenotyp mutacji termowrażliwych (Effect of molecular chaperones on the temperature sensitive mutant phenotypes) Training: molecular biology Molecular chaperones Master thesis: Wpływ poziomu ekspresji białek opiekuńczych na fenotyp mutacji termowrażliwych (Effect of molecular chaperones on the temperature sensitive mutant phenotypes) Training: molecular biology

Research Internship (Erasmus)

• Oct 2008 - Feb 2009

Rituximab & trogocytosis Research internship thesis: ‘Trogocytosis’ during antibody therapy against B-cell malignances. Which mechanisms are involved? Training: immunology Rituximab & trogocytosis Research internship thesis: ‘Trogocytosis’ during antibody therapy against B-cell malignances. Which mechanisms are involved? Training: immunology

Summer Internship

• Jul 2008 - Sep 2008

RNA processing Training: molecular biology RNA processing Training: molecular biology

Volunteer Research Assistant

• Oct 2007 - Jan 2008

- CD8+ cells: identification, isolation and culture - Flow cytometry - Tumor immunology - CD8+ cells: identification, isolation and culture - Flow cytometry - Tumor immunology

Summer Internship

• Jul 2007 - Sep 2007

Therapeutic genetically modified melanoma vaccines Training: cell biology, molecular biology Therapeutic genetically modified melanoma vaccines Training: cell biology, molecular biology