Experience

The Sainsbury Laboratory

• United Kingdom
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Assistant

  • Dec 2020 - Present

• Laboratory Technician

  • Jul 2017 - Nov 2020



The James Hutton Institute

• United Kingdom
• Research Services
• 200 - 300 Employee

• PHD Student

  • Apr 2013 - Jun 2017

  Responses of the Phytophthora infestans metabolome and transcriptome to 'mock infection' and chemical inhibition. Responses of the Phytophthora infestans metabolome and transcriptome to 'mock infection' and chemical inhibition.



The University of Dundee

• 1 - 100 Employee

• Demonstrator

  • Jan 2016 - Apr 2016

  Demonstrator for undergraduates during their practicals. My role was to help students when needed during practicals, asking questions to make sure they understood what they were working on and helping them figuring out the answers. It was also to ensure that student were following the safety guides. Demonstrator for undergraduates during their practicals. My role was to help students when needed during practicals, asking questions to make sure they understood what they were working on and helping them figuring out the answers. It was also to ensure that student were following the safety guides.



UMR-USTL-INRA, Lille.

• Master Thesis Project

  • Jan 2012 - Jul 2012

  Cell suspension cultures of chicory roots are used to product compounds of interest and study metabolic pathways. It has been demonstrated that the ratio between 5-mono-O-caffeoylquinicacid (5CQA) and 3,5-di-O-caffeoylquinic acid (3,5diCQA) observed in Chicory roots (Cichorium intybus var. sativum) extracts is reversed in cell suspension culture initiated from Chicory roots. In the latter, 3,5diCQA contents (6.24 µmol/g DW) are 3 fold higher than 5CQA (2.05 µmol/g DW). Thus this model could provide information about the biosynthetic pathway of 3,5diCQA which still need to be elucidated. For this purpose, we investigated conditions giving contrasting 3,5diCQA/CQA ratios by eliciting cells with methyljasmonate (MeJa), a known activator of the phenylpropanoid pathway. Phenolic acids were extracted and quantified using LC-DAD. At 50µM MeJa, the content of 3,5diCQA increased up to 26 fold and the content of 5CQA up to 40 fold, seven days after MeJa elicitation. Extracts were also analyzed by LC-ESI-MSn. Mass spectrometry data were analyzed to get more information about the evolution of global metabolism in presence of MeJa. We have completed our study with a comparison of the expression levels variations of 6 genes involved in metabolic pathway from phenylalanine to 5CQA (HQT, HCT, PAL1, C4H, C4L, C3H). The expression of 4 of these genes increased following the addition of Meja (HCT, PAL1, C4H, C4L). These results confirm this experimental system and its use for a deepest transcriptomic analysis.



UMR-UTSL-INRA

• Student Internship

  • Jan 2010 - Apr 2010

  Media preparation, solid and liquid. Micropropagation of different plant species, including Miscanthus, Chicory, Cactus, etc...Transfer from in vitro to soil. Media preparation, solid and liquid. Micropropagation of different plant species, including Miscanthus, Chicory, Cactus, etc...Transfer from in vitro to soil.



URIANE

• France

• Summer Internship

  • Jun 2009 - Aug 2009

  Dairy product microbiological analysis (cheese and milk).Daily Routine Analysis in sterile conditions.Preparation of agar plates and liquid media. Reception and organisation of delivery. Cleaning and sterilization of lab equipment. Dairy product microbiological analysis (cheese and milk).Daily Routine Analysis in sterile conditions.Preparation of agar plates and liquid media. Reception and organisation of delivery. Cleaning and sterilization of lab equipment.