Experience

1859

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist: Automated Chemistry

  • Dec 2021 - Sep 2022

  Leveraged expertise in programming to optimize laboratory workflows, successfully programming multiple liquid handling robots for use in chemistry and molecular biology applications. Designed and fabricated custom modules for use with both ALD3 SPE robots and Tecan EVO liquid handlers, expanding the robots' capabilities and enabling more efficient sample processing. Demonstrated innovation and problem-solving skills by adapting various labware components to the liquid handling… Show more Leveraged expertise in programming to optimize laboratory workflows, successfully programming multiple liquid handling robots for use in chemistry and molecular biology applications. Designed and fabricated custom modules for use with both ALD3 SPE robots and Tecan EVO liquid handlers, expanding the robots' capabilities and enabling more efficient sample processing. Demonstrated innovation and problem-solving skills by adapting various labware components to the liquid handling robot decks, allowing for more versatile and precise sample handling. Showcased technical proficiency by creating custom valve control boards, mixers, and heaters, which enhanced the performance of the liquid handling robots and allowed for greater control over the experimental parameters. Developed Python scripts to control and monitor process set-points of custom hardware built with Arduino, streamlining the experimental workflow and ensuring consistent, high-quality results.

• Associate Scientist: engineering

  • Sep 2020 - Feb 2022

  Spearheaded the design and implementation of several innovative modules that significantly improved the functionality and stability of an alpha Microfluidic Instrument, including: PID-regulated Thermoelectric coolers for maintaining long-term stability of thermolabile reagents, resulting in more consistent and accurate experimental results. Vibration and mixing module for ensuring long-term and stable slurry delivery into Microfluidic chips, which improved the reproducibility of… Show more Spearheaded the design and implementation of several innovative modules that significantly improved the functionality and stability of an alpha Microfluidic Instrument, including: PID-regulated Thermoelectric coolers for maintaining long-term stability of thermolabile reagents, resulting in more consistent and accurate experimental results. Vibration and mixing module for ensuring long-term and stable slurry delivery into Microfluidic chips, which improved the reproducibility of the experimental data. Pneumatic reagent caps for reagent reservoirs, reagent mounts, syringe holders, and electrodes for High voltage dielectrophoresis, which allowed for faster experimental setups. Successfully set up and maintained the pneumatics and custom optics, including the PMT emission train and laser excitation train, of the microfluidic rig, ensuring the accuracy and reliability of the experimental results. Demonstrated exceptional proficiency in CAD modeling by creating a comprehensive SolidWorks assembly of the entire microfluidic rig, including the custom optical train, providing a clear visualization of the experimental setup and facilitating efficient troubleshooting and optimization of the system

• Research Associate: Integration

  • Nov 2019 - Sep 2020

  Fabricated PDMS-glass microfluidic devices using softlithography from SU-8 patterned Si wafers. Bonded devices via plasma mediated surface activation of the PDMS's silanol groups. evaluated and characterized several droplet microfluidic devices to be used in kinetic screening and assay characterization. -analyzed droplet data via Python



Plexium, Inc.

• Engineer

  • Jun 2019 - Aug 2019

• Research Associate and Lab Manager

  • Jan 2018 - Aug 2019



UC San Diego

• United States
• Higher Education
• 700 & Above Employee

• Lab Assistant 2

  • Nov 2015 - Aug 2017

  Tissue culture, recombinant DNA building/screening in bacteria(E.coli), AAV production in tissue culture, AAV purification via chromatography. Quantification of various biomolecules using spectroscopic methods. In charge of general supply ordering, lab safety, and general lab organization. Also, help with various tech related problems. Sometimes this involves constructing microcontroller based circuits from self developed ideas as well as extensive use of open source designs. Tissue culture, recombinant DNA building/screening in bacteria(E.coli), AAV production in tissue culture, AAV purification via chromatography. Quantification of various biomolecules using spectroscopic methods. In charge of general supply ordering, lab safety, and general lab organization. Also, help with various tech related problems. Sometimes this involves constructing microcontroller based circuits from self developed ideas as well as extensive use of open source designs.



Scripps Research

• United States
• Research Services
• 700 & Above Employee

• Research Technician/ Lab manager

  • Jan 2014 - Nov 2015

  Cell culture and Dj AAV production: Maintained Hek293t cell line for recombinant AAV production. This involved building AAV constructs by molecular cloning, transformation of cell cultures, harvesting cells for extraction, and purifying the recombinant AAV via affinity chromatography with heparin columns. Cloning: Helped in the Developent of AAV shuttle vectors for department use for various optogenetic/ reporter gene experiments in molecular neurobiology. Also… Show more Cell culture and Dj AAV production: Maintained Hek293t cell line for recombinant AAV production. This involved building AAV constructs by molecular cloning, transformation of cell cultures, harvesting cells for extraction, and purifying the recombinant AAV via affinity chromatography with heparin columns. Cloning: Helped in the Developent of AAV shuttle vectors for department use for various optogenetic/ reporter gene experiments in molecular neurobiology. Also, involved in several molecular biology projects for developing new transgenic mice lines. Management of Transgenic mice Genotyping: Trained and supervised interns working on pcr based genotyping for current transgenic mice colony. Also, maintained records of genotypes for mice in colony for lab personnel via an excel spreadsheet Lab manger duties: In charge of general organization of lab supplies, equipment, ordering, and maintaining current protocols for personnel. Side projects: Development of Microcontroller based automation equipment for lab personnel to use in experiments; such as, several led controllers used for optigenetics in my lab. For example, using a microcontroller as a precision timer and a relay circuit I switched a waveform generators output to an led that stimulates neurons via a transgenic channel rhodopsin protein in mice. I'm currently working on making a cheap automatic chromatography system for my virus purification efforts. I plan on using an Arduino Due and interfacing it with a Masterflex precision peristaltic pump via a 16bit precision 4 to 20mA digital to analog converter to control the speed of the pump and its direction.

• Research Intern

  • Feb 2013 - Jan 2014

  Mice Genotyping: Performed DNA extraction, PCR amplification, Agarose gel Electrophoresis, and interpretation of genotypes. Designed Excel sheets for mice genotype record keeping and supervised other interns in keeping current records of genotypes for mice in colony. Designed primers and optimized their use in the labs standard PCR protocol. Provided collaborators with up to date protocols, and helped troubleshoot problems with their genotyping efforts. Recombinant protein… Show more Mice Genotyping: Performed DNA extraction, PCR amplification, Agarose gel Electrophoresis, and interpretation of genotypes. Designed Excel sheets for mice genotype record keeping and supervised other interns in keeping current records of genotypes for mice in colony. Designed primers and optimized their use in the labs standard PCR protocol. Provided collaborators with up to date protocols, and helped troubleshoot problems with their genotyping efforts. Recombinant protein development: Under minimal supervision successfully synthesized DNA constructs via overlap extension PCR (OEPCR) and using various recombinant techniques including: plasmid extraction, enzymatic DNA manipulation, bacteria transformation, and sequence analysis. Successfully performed plasmid growth in DH5-alpha and STBL E. coli strains. Successfully grew and extracted protein from BL21 E. coli strains using pET-15b based recombinant plasmids. Utilized troubleshooting skills to optimize methods for difficult recombinant Protein Extractions/Purifications using Flash/affinity Column Chromatography, SDS-Page, and spectroscopic quantification methods. Began conducting experiments to implement a Prokaryotic Cell free protein expression protocol for use in future projects in hopes to reduce extraction times for higher throughput experiments. Other side projects/duties: Made some program in the R environment for the Post-Doc to randomize file names for unbiased image data analysis. Helped in general organization of lab supplies and ordered personal/general lab supplies when needed. Performed some maintenance on laboratory equipment such as fixing broken micropipettes and fixing a rotary oven.



Briggs lab: UC San Diego

• La Jolla,CA

• Undergraduate research volunteer

  • Jun 2013 - Dec 2014

  LCMS based studies of post translational protein modifications in arabidopsis: Under minimal supervision performed Arabidopsis protein extraction, purification, and trypsin digestions of proteins to make Tryptic peptides for Proteomic research. Quantified peptides via spectroscopic methods and added various standards for downstream quantification. Operated a triple Quad LC/MS system and prepared reverse phase nanoflow columns for use in peptide separation. Under guidance from a graduate… Show more LCMS based studies of post translational protein modifications in arabidopsis: Under minimal supervision performed Arabidopsis protein extraction, purification, and trypsin digestions of proteins to make Tryptic peptides for Proteomic research. Quantified peptides via spectroscopic methods and added various standards for downstream quantification. Operated a triple Quad LC/MS system and prepared reverse phase nanoflow columns for use in peptide separation. Under guidance from a graduate student, helped in an MRM assay development for acetylated proteins of interest: via optimizing peptide product ion selections at the MS 2 detector, performing collision Energy optimization for peptide ion fragmenting, and helped run samples in the final MRM ASSAY. Show less LCMS based studies of post translational protein modifications in arabidopsis: Under minimal supervision performed Arabidopsis protein extraction, purification, and trypsin digestions of proteins to make Tryptic peptides for Proteomic research. Quantified peptides via spectroscopic methods and added various standards for downstream quantification. Operated a triple Quad LC/MS system and prepared reverse phase nanoflow columns for use in peptide separation. Under guidance from a graduate… Show more LCMS based studies of post translational protein modifications in arabidopsis: Under minimal supervision performed Arabidopsis protein extraction, purification, and trypsin digestions of proteins to make Tryptic peptides for Proteomic research. Quantified peptides via spectroscopic methods and added various standards for downstream quantification. Operated a triple Quad LC/MS system and prepared reverse phase nanoflow columns for use in peptide separation. Under guidance from a graduate student, helped in an MRM assay development for acetylated proteins of interest: via optimizing peptide product ion selections at the MS 2 detector, performing collision Energy optimization for peptide ion fragmenting, and helped run samples in the final MRM ASSAY. Show less



UC San Diego

• United States
• Higher Education
• 700 & Above Employee

• Student

  • Sep 2010 - Dec 2013

  Studying Biochemistry in the Chemistry Department, and Studying Human Biology in the Biology Department

• student volunteer

  • Jan 2013 - Feb 2013

  helped scan micrographs for 3D reconstructions of symmetric viral envelopes