Research Associate

• Apr 2021 - Present

• Metabolite profiling from different types of biological matrices (biofluids, cell lines, bacterial cultures) using GC-MS and LC-MS/MS-QTOF • Contributing to set-up of standardized protocols for sample preparation • Operating and troubleshooting chromatographic/mass spectrometry equipment • Carrying out sample analyses using the targeted and/or untargeted approach • Contributing to set-up of standardized LC-MS methods for targeted analysis • Performing data analysis using the existing bioinformatics platform (MetaboAnalyst) • Keeping an up to date written and electronic record of laboratory work • Contributing to maintenance of adequate stocks in the laboratory (consumables such as chemicals, gases, disposables) • Helping ensure the functionality of key platform apparatus (service/maintenance) by liaising with the platform staff in a timely manner Show less

Research Trainee

• Sep 2020 - Dec 2020

Doctoral Student

• Jan 2013 - Dec 2019

𝐓𝐢𝐭𝐥𝐞: Machine Learning Based Evaluation of Human Hypothetical Proteins and Validation of Their Expression. 𝐓𝐡𝐞𝐬𝐢𝐬 𝐰𝐨𝐫𝐤: My PhD work mainly focused on studying human hypothetical proteins (HPs), a group of proteins for which there is no experimental evidence of translation and functional characterization. Different bioinformatics tools and methods were used for the functional annotation of hypothetical/uncharacterized protein sequences. Different available functional annotation methods were evaluated using machine learning based models. Later, to check whether or not these sequences are translatable, proteomics based approach was used to provide the analytical basis of their identification. My study established most of these hypothetical sequences as non-coding RNAs and the products of pseudogenes rather than proteins. However, confirming the expression of few HPs at protein level made it clear that these 'hypothetical' or 'uncharacterized' proteins cannot be completely ignored. PMID: 25873935 PMID: 30621574 https://doi.org/10.1007/s42485-020-00030-1 Show less

Guest Researcher

• Jan 2019 - Jun 2019

Project: Identification and Characterization of Human Hypothetical Proteins from Cancer Cell lines using proteomics and bioinformatics approaches. • Sample preparation for proteomics • Purification of proteins using SDS-PAGE and 2D gel electrophoresis • Protein profiling using LC-MS/MS methods (Thermo Q Exactive HF) • Label free quantification Project: Identification and Characterization of Human Hypothetical Proteins from Cancer Cell lines using proteomics and bioinformatics approaches. • Sample preparation for proteomics • Purification of proteins using SDS-PAGE and 2D gel electrophoresis • Protein profiling using LC-MS/MS methods (Thermo Q Exactive HF) • Label free quantification

Summer Research Fellow

• May 2018 - Jul 2018

Project: Identification of differentially expressed genes in Staphylococcus aureus in response to antibiotic exposure. Project: Identification of differentially expressed genes in Staphylococcus aureus in response to antibiotic exposure.

Teaching Assistant

• Sep 2015 - Sep 2017

Taught Biochemistry, Cell biology, and Molecular biology courses to MSc students. Taught Biochemistry, Cell biology, and Molecular biology courses to MSc students.